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Regulation of initiation of chromosome replication

Research Project

Project/Area Number 08277101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionGraduate School of Science, Osaka University

Principal Investigator

TAKISAWA Haruhiko  Graduate School of Science, Osaka University Professor, 大学院・理学研究科, 教授 (60154944)

Co-Investigator(Kenkyū-buntansha) MASUKATA Hisao  Graduate School of Science, Osaka University, Professor, 大学院・理学研究科, 教授 (00199689)
SHINOMIYA Tomoyuki  Mitsubishi Kase Institute of Life Sciences, Project Leader Scientist, 主任研究員
MATSUKAGE Akio  Graduate School of Science, Japan Women's University, Professor, 理学部, 教授 (90019571)
SHIRAHIGE Katsuhiko  Graduate School of Biological Sciences, Nara Advanced Institute of Science and Technology, Associate Professor, バイオサイエンス研究科, 助教授 (90273854)
MASAI Hisao  Institute of Medical Science, University of Tokyo, Associate Professor, 医科学研究所・分子生物学研究部, 助教授 (40229349)
片山 勉  九州大学, 薬学部, 助手 (70264059)
Project Period (FY) 1996 – 1999
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥213,500,000 (Direct Cost: ¥213,500,000)
Fiscal Year 1999: ¥50,800,000 (Direct Cost: ¥50,800,000)
Fiscal Year 1998: ¥52,600,000 (Direct Cost: ¥52,600,000)
Fiscal Year 1997: ¥55,000,000 (Direct Cost: ¥55,000,000)
Fiscal Year 1996: ¥55,100,000 (Direct Cost: ¥55,100,000)
Keywordschromosome replication / replication origin / replication initiation apparatus / regulation of initiation / replication-related gene / DNA複製 / MCM / ORC / CDC7 / CDC45 / 染色体 / DNAポリメラーゼ / 複製必須配列 / 複製開始複合体 / Cdc7-Dbf4キナーゼ / P1蛋白質 / DNA複製遺伝子の発現制御 / 転写制御因子間相互作用 / DNA複製開始領域 / DNA複製開始蛋白質 / P1ファミリー蛋白質 / CDC7類似キナーゼ / 複製関連遺伝子 / 転写調節因子 / DREF
Research Abstract

The ultimate goal of this research proposal is to understand the mechanisms of regulation of replication initiation in both prokaryotes and eukaryotes.
This research group has investigated three aspects of replication initiation ; the origin of replication, the components of replication initiation, and the regulation of initiation. During this research period, origin regions of replication were precisely analyzed in chromosomes of Schizosaccharomyces pombe, and Drosophila melanogaster. In Saccharomyces cerevisiae, the firing timing of every origins on a whole chromosome was thoroughly investigated, revealing the control mechanisms of initiation of DNA replication at chromosomal level. As to the components of replication initiation, homologues of proteins (ORC, MCM, Cdc45, etc.) essential for initiation of chromosome replication have been shown to be conserved in eukaryotic species. In particular, Cdc45 has been found to play a crucial role in the unwinding of origin DNA and recruiting DNA polymerase alpha onto the unwound DNA.As to the regulation of initiation, S-phase promoting kinases have been shown to play a universal role in the regulation of initiation through the phosphorylation of target proteins. With Drosophila melanogaster, two types of transcription factors have been shown to be involved in the trasncription of replication-related genes. Further, studies on regulatory mechanisms of replication initiation in E.coli reveals that the function of initiator protein is regulated by DNA polymerase.

Report

(5 results)
  • 2000 Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (139 results)

All Other

All Publications (139 results)

  • [Publications] Mizushima,T., N.Takahashi, et al.: "Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro."Genes Dev. 14. 1631-1641 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mimura,S., T.Masuda, et al.: "Central role for cdc45 in establishing an initiation complex of DNA replication in Xenopus egg extracts."Genes Cells. 5. 439-452 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka, K., T.Yonekawa, et al.: "Fission yeast Esolp is required for establishing sister chromatid cohesion during S phase."Mol Cell Biol. 20. 3459-3469 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Uga,H., F.Matsunaga, et al.: "Regulation of DNA replication by iterons : an interaction between the ori2 and incC regions mediated by RepE -bound iterons inhibits DNA replication of mini F plasmid in Escherichia coli."EMBO J. 18. 3856-3867 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogawa,Y., T.Takahashi, et al.: "Association of fission yeast Orp1 and Mcm6 proteins with chromosomal replication origins."Mol Cell Biol. 19. 7228-7236 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kohzaki,H., Y.Ito, et al.: "Context-dependent modulation of replication activity of Saccharomyces cerevisiae autonomously replicating sequences by transcription factors."Mol Cell Biol. 19. 7428-7435 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirose,F., M.Yamaguchi, et al.: "Targeted expression of the DNA binding domain of DRE-binding factor, a Drosophila transcription factor, attenuates DNA replication of the salivary gland and eye imaginal disc."Mol Cell Biol. 19. 6020-6028 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasaki,T., T.Sawado, et al.: "Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster."Mol Cell Biol. 19. 547-555 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Komori,H., F.Matsunaga, et al.: "Crystal structure of a prokaryotic replication initiator protein bound to DNA at 2.6 A resolution."EMBO J. 18. 4597-4607 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeda,T., K.Ogino, et al.: "A fission yeast gene, him1(+)/dfp1(+), encoding a regulatory subunit for Hsk1 kinase, plays essential roles in S-phase initiation as well as in S-phase checkpoint control and recovery from DNA damage."Mol Cell Biol. 19. 5535-5547 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujita,M., C.Yamada, et al.: "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation."J Biol Chem. 274. 25927-25932 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kurokawa,K., S.Nishida, et al.: "Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli."EMBO J. 18. 6642-6652 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirose,F., M.Yamaguchi, et al.: "A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S tratasition in mammalian cells."Mol Cell Biol. 19. 5083-5095 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasaki,T., T.Sawado, et al.: "Clustered adenine/thymine stretches are essential for function of a fission yeast replication origin."Mol Cell Biol. 19. 6699-6709 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Komori,H., F.Matsunaga, et al.: "A DNA unwinding factor involved in DNA replication in cell-free extracts of Xenopus eggs."EMBO J. 9. 341-350 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeda,T., K.Ogino, et al.: "Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk."Mol Cell Biol. 17. 5699-5707 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujita,M., C.Yamada, et al.: "Functional domains of rep2, a transcriptional activator subunit for Res2-Cdc 10, controlling the cell cycle "start"."J Biol Chem. 9. 1577-1588 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kurokawa,K., S.Nishida, et al.: "Regulation of DNA-replication origins during cell-cycle progression."EMBO J. 395. 618-621 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujita,M., Y.Hori, et al.: "Cell cycle dependent topological changes of chromosomal replication origins in Saccharomyces cerevisiae."Genes Cells. 3. 737-749 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katayama,T., T.Kubota, et al.: "The initiator function of DnaA protein is negatively regulated by the sliding clamp of the E.coli chromosomal replicase."Cell. 94. 61-71 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akamatsu,E., T.Tanaka, et al.: "Transcription factor E2F and cyclin E-Cdk2 complex cooperate to induce chromosomal DNA replication in Xenopus oocytes."J Biol Chem. 273. 16494-16500 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamashita,M., Y.Hori, et al.: "The efficiency and timing of initiation of replication of multiple replicons of Saccharomyces cerevisiae chromosome VI."Genes Cells. 2. 655-665 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato,N., K.Arai, et al.: "Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases : in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7."EMBO J. 16. 4340-4351 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kubota,Y., S.Mimura, et al.: "Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs."EMBO J. 16. 3320-3331 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hayashi,Y., F.Hirose, et al.: "Identification of CFDD (common regulatory factor for DNA replication and DREF genes) and role of its binding site in regulation of the proliferating cell nuclear antigen gene promoter."J Biol Chem. 272. 22848-22858 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hori,Y., K.Shirahige, et al.: "Characterization of a novel CDC gene (ORC1) partly homologous to CDC6 of Saccharomyces cerevisiae."Mol Biol Cell. 7. 409-418 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirose,F., M.Yamaguchi, et al.: "Isolation and characterization of cDNA for DREF, a promoter-activating factor for Drosophila DNA replication-related genes."J Biol Chem. 271. 3930-3937 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Obuse,C., Y.Okuno, et al.: "A replication-enhancing element with transcriptional silencer activity in autonomously replicating human chromosomal fragments."Mol Biol Cell. 7. 43-55 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murakami,Y., J.A.Huberman, et al.: "Identification, purification, and molecular cloning of autonomously replicating sequence-binding protein 1 from fission yeast Schizosaccharomyces pombe."Proc Natl Acad Sci USA. 93. 502-507 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mizushima, T., N.Takahashi and B.Stillman: "Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro."Genes Dev. 14. 1631-1641 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mimura, S., T.Masuda, T.Matsui and H.Takisawa: "Central role for cdc45 in establishing an initiation complex of DNA replication in Xenopus egg extracts."Genes Cells. 5. 439-452 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tanaka, K., T.Yonekawa, Y.Kawasaki, M.Kai, K.Furuya, M.Iwasaki, H.Murakami, M.Yanagida and H.Okayama: "Fission yeast Esolp is required for establishing sister chromatid cohesion during S phase."Mol Cell Biol. 20. 3459-3469 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Uga, H., F.Matsunaga and C.Wada: "Regulation of DNA replication by iterons : an interaction between the ori2 and incC regions mediated by RepE-bound iterons inhibits DNA replication of miniF plasmid in Escherichia coli."EMBO J. 18. 3856-3867 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ogawa, Y., T.Takahashi and H.Masukata: "Association of fission yeast Orp1 and Mcm6 proteins with chromosomal replication origins."Mol Cell Biol. 19. 7228-7236 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kohzaki, H., Y.Ito and Y.Murakami: "Context-dependent modulation of replication activity of Saccharomyces cerevisiae autonomously replicating sequences by transcription factors."Mol Cell Biol. 19. 7428-7435 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirose, F., M.Yamaguchi and A.Matsukage: "Targeted expression of the DNA binding domain of DRE-binding factor, a Drosophila transcription factor, attenuates DNA replication of the salivary gland and eye imaginal disc."Mol Cell Biol. 19. 6020-6028 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sasaki, T., T.Sawado, M.Yamaguchi and T.Shinomiya: "Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster."Mol Cell Biol. 19. 547-555 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Komori, H., F.Matsunaga, Y.Higuchi, M.Ishiai, C.Wada and K.Miki: "Crystal structure of a prokaryotic replication initiator protein bound to DNA at 2.6 A resolution."EMBO J. 18. 4597-4607 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeda, T., K.Ogino, E.Matsui, M.K.Cho, H.Kumagai, T.Miyake, K.Arai and H.Masai: " A fission yeast gene, him1 (+)/dfp1 (+), encoding a regulatory subunit for Hsk1 kinase, plays essential roles in S-phase initiation as well as in S-phase checkpoint control and recovery from DNA damage."Mol Cell Biol. 19. 5535-5547 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujita, M., C.Yamada, H.Goto, N.Yokoyama, K.Kuzushima, M.Inagaki and T.Tsurumi: "Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation."J Biol Chem. 274. 25927-25932 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kurokawa, K., S.Nishida, A.Emoto, K.Sekimizu and T.Katayama: "Replication cycle-coordinated change of the adenine nucleotide-bound forms of DnaA protein in Escherichia coli."EMBO J. 18. 6642-6652 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumagai, H., N.Sato, M.Yamada, D.Mahony, W.Seghezzi, E.Lees, K.Arai and H.Masai: "A novel growth- and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition in mammalian cells."Mol Cell Biol. 19. 5083-5095 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okuno, Y., H.Satoh, M.Sekiguchi and H.Masukata: "Clustered adenine/thymine stretches are essential for function of a fission yeast replication origin."Mol Cell Biol. 19. 6699-6709 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Okuhara, K., K.Ohta, H.Seo, M.Shioda, T.Yamada, Y.Tanaka, N.Dohmae, Y.Seyama, T.Shibata and H.Murofushi: "A DNA unwinding factor involved in DNA replication in cell-free extracts of Xenopus eggs."Curr Biol. 9. 341-350 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mimura, S.and H.Takisawa: "Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk."EMBO J. 17. 5699-5707 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tahara, S., K.Tanaka, Y.Yuasa and H.Okayama: "Functional domains of rep2, a transcriptional activator subunit for Res2-Cdc 10, controlling the cell cycle "start"."Mol Biol Cell. 9. 1577-1588 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shirahige, K., Y.Hori, K.Shiraishi, M.Yamashita, K.Takahashi, C.Obuse, T.Tsurimoto and H.Yoshikawa: "Regulation of DNA-replication origins during cell-cycle progression."Nature. 395. 618-621 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujita, M., Y.Hori, K.Shirahige, T.Tsurimoto, H.Yoshikawa and C.Obuse: "Cell cycle dependent topological changes of chromosomal replication origins in Saccharomyces cerevisiae."Genes Cells. 3. 737-749 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katayama, T., T.Kubota, K.Kurokawa, E.Crooke and K.Sekimizu: "The initiator function of DnaA protein is negatively regulated by the sliding clamp of the E.coli chromosomal replicase."Cell. 94. 61-71 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akamatsu, E., T.Tanaka and J.Y.Kato: "Transcription factor E2F and cyclin E-Cdk2 complex cooperate to induce chromosomal DNA replication in Xenopus oocytes."J Biol Chem. 273. 16494-16500 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamashita, M., Y.Hori, T.Shinomiya, C.Obuse, T.Tsurimoto, H.Yoshikawa and K.Shirahige: "The efficiency and timing of initiation of replication of multiple replicons of Saccharomyces cerevisiae chromosome VI."Genes Cells. 2. 655-665 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sato, N., K.Arai and H.Masai: "Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases : in vitro phosphorylation of MCM subunits by a putative human homologue of CdC7."EMBO J. 16. 4340-4351 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kubota, Y., S.Mimura, S.Nishimoto, T.Masuda, H.Nojima and H.Takisawa: "Licensing of DNA replication by a multi-protein complex of MCM/Pl proteins in Xenopus eggs."EMBO J. 16. 3320-3331 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hayashi, Y., F.Hirose, Y.Nishimoto, M.Shiraki, M.Yamagishi, A.Matstukage and M.Yamaguchi: "Identification of CFDD (common regulatory factor for DNA replication and DREF genes) and role of its binding site in regulation of the proliferating cell nuclear antigen gene promoter."J Biol Chem. 272. 22848-22858 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hori, Y., K.Shirahige, C.Obuse, T.Tsurimoto and H.Yoshikawa: "Characterization of a novel CDC gene (ORC1) partly homologous to CDC6 of Saccharomyces cerevisiae."Mol Biol Cell. 7. 409-418 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hirose, F., M.Yamaguchi, K.Kuroda, A.Omori, T.Hachiya, M.Ikeda, Y.Nishimoto and A.Matsukage: "Isolation and characterization of cDNA for DREF, a promoter-activating factor for Drosophila DNA replication-related genes."J Biol Chem. 271. 3930-3937 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Obuse, C., Y.Okuno, T.Okazaki and H.Masukata: "A replication-enhancing element with transcriptional silencer activity in autonomously replicating human chromosomal fragments."Mol Biol Cell. 7. 43-55 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Murakami, Y., J.A.Huberman and J.Hurwitz: "Identification, purification, and molecular cloning of autonomously replicating sequence-binding protein 1 from fission yeast Schizosaccharomyces pombe."Proc Natl Acad Sci USA. 93. 502-507 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Mimura, S.: "Central role for Cdc45 in establishing an initiation compex of DNA replication in Xenopus egg extracts"Genes Cells in press.

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohta, K.: "Action of a new mammalian DNA polymer ase inhibitor, sulfoquinovosl diacylglycerol"Biol. Pharm. Bull.. 22. 111-116 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takahashi, Y.: "Identification of three conserved regions in the DREF transcription factors from Drosophila melanogaster and Drosophila virilis"Nucleic Acids Res.. 27. 510-516 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hirose, F.: "Targeted expression of the DNA binding domain of Drosophila transcription factor DREF attenuates DNA replication"Mol. Cell. Biol.. 18. 6020-6028 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mizushima, Y.: "The cyanogenic glucoside, prenasin (D-mandelorritril-D-glucoside), is a novel inhibitor of DNA polymerase b"J. Biochem.. 126. 430-436 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamaguchi, M.: "Ectopic expression of human p23 inhibits entry into S-phase and induces apoptosis in the Drosophila eye imaginal disc"Oncogene. 18. 6767-6775 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hayashi, Y.: "A binding site for transcription factor Granyhead/nuclear transcription factor-1 contributes to regulation"J. Biol. Chem.. 274. 35080-35088 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masai, H.: "Regulation of DNA replication duringsell sysle and environmental stresses"IUBMB Life, in press.

    • Related Report
      1999 Annual Research Report
  • [Publications] Masai, H.: "Cdc7/Dbf4-related kinase complex as a molecular switch for initiation of DNA replication"Frontiers in Bioscience. 4. 834-840 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takada, T.: "A Fission yeast gane, him1+/dfp1+, encoding a regulatory subunit for Hsk1 kinase, play essential roles in S phase initiation"Mol. Cell. Biol.. 19. 5535-5547 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masai, H.: "Escherichia coil and Bacillus subtilis PriA proteins essential for recombination-dependent DNA replication : involvement of ATPase"Biochimie. 81. 847-857 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kumagai, H.: "A novel growth-and cell cycle-regulated protein, ASK, activates human Cdc7-related kinase and is essential for G1/S transition"Mol. Cell. Biol.. 19. 5083-5095 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Johnstone, L.: "First the Cdk's, now the Ddk's"Trends in Cell Biology. 9. 249-252 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Okuno: "Clustered adenine/thymine stretches are essential for the function of a fission yeast replication orgin"Mol. Cell. Biol.. 19・10. 6699-6709 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y. Ogawa: "Association of fission yeast Orp1 and Mcm6 proteins with chromosomal replication origins"Mol. Cell. Biol.. 19・10. 7228-7236 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masuda, M.: "Genetic studies with the fission yeastSchizosaccharomyces pombe suggest involvement of Wee1, Ppa2, and Rad24 in induction of cell"J. Virol.. 74 (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Koichi Tanaka: "Fission Yeast Eso1p is required for establishing sister chromatid cohesion during S phase"Mol. Cell. Biol.. 20 (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Shirahige, K.: "The Cloning and characterization of CDC50 gane family in S. cerevisiae"Yeast. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Takata, M.: "MutantDnaA prteins defective in opening of oriC, the origin of chromosomal DNA replication in Escherichia coil"Mol. Microbiol.. 35・2. 454-462 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kurokawa, K.: "Replication cycle-coordinated change of the adenine nusleotide-bound forms of DnaA Protein in Escherichia coli"ENBO J.. 18・23. 6642-6652 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Guo, L.: "Isolation and characterization of novel cold-sensitive dnaA mutants of Escherichia coli"FEMS Microbiol. lett.. 176. 357-366 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katayama, T.: "Inactivation of Escherichia coli DnaA protein by DNA polymerase III, and negative regulations for initiation of chromosomal replication"Biochimie. 81・8. 835-840 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mimura,S.: "Xenopus Cdc45-dependent loading of DNA polymerase α onto chromatin under the control of S-phase cdk." EMBO,J.17. 5699-5707 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Katayama,T.: "The initiator function of DnaA protein is negatively regulated by the sliding clamp of the E.coli chromosomal replicase." Cell. 94(1). 61-71 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kurokawa,K.: "A stimulation factor for hydrolysis of ATP bound to DnaA protein,the initiator of chromosomal DNA replication in Escherichia coli." Biochem.Biophys.Res.Commun.243(1). 90-95 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizushima,T.: "Site-directed mutational analysis for the ATP binding of DnaA protein: functions of two conserved amino acids (Lys-178 and Asp-235)located" J.Biol.Chem.273(33). 20847-20851 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sawado,T.: "The DNA replication-related element (DRE)/ DRE-binding factor system is a transcriptiona regulator ofthe Drosophila E2F gene." J.Biol.Chem.273. 26042-26051 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sawado,T.: "dE2F, a novel E2F-family transcription factor in Drosophila melanogaster." Biochem.Biophys.Res.Commun.251. 409-415 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] C.Obuse: "Interaction of transcription factor YY1 with a replication-enhancing element,REE1,in an autonomously replicating human chromosome" Nucle.Acids Res.1. 253-253 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sasaki,T.: "Specification of Initation Regions during Embryogenesis in the 65 kilobase DNA pol α-dE2F Locus of Drosophila melanogaster." Mol.Cel.Biol.,. 19,1. 547-555 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Ogawa,T: "Association of autonomous replication activity with replication origins in a human chromosome." Exp.Cell Res.243. 50-58 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Horie,S.: "The Schizosaccharomyces pombe mei4+ gene encodes a meiosis-specific transcription factor containing a forkhead" Mol.Cell.Biol.18. 2118-2129 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tahara,S.: "Functional domains of Rep2,a transcriptional activator subunit for Res2-Cdc10,controlling the cell cycle“start"." Mol.Biol.Cell.9. 1577-1588 (1988)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shirahige,K: "Regulation of DNA replication origins during cell-cycle progression." Nature. 395. 618-621 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Horie,T.: "A 5'-monophosphate form of bredinin selectively inhibits the activities of mammalian DNA polymerases in vitro." Intn.J.Mol.Med.1. 83-90 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ohta,K.: "Sulfoquinovosyldiacylglycerol,KM043,a new potent inhibitor of eukaryotic DNA polymerases and HIV-reverse transcriptase type I from" Gigartina tenella.Chem.Pharm.Bull.46. 684-686 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizushina,Y.: "The inhibitory effect of novel triterpenoid compounds,fomitellic acids, on DNA polymerase b." Biochem.J.330. 1325-1332 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizushina,Y.: "The biochemical inhibition mode of bredinin-5'-monophosphate on DNA polymerase b.Biochim." Biophys.Acta.1403. 5-11 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ohno,K.: "cDNA cloning and expression during development of Drosophila melanogaster MCM3,MCM6 and MCM7." Gene. 217. 177-186 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizushina,Y.: "A mushroom fruiting body-inducing substance inhibits activities of replicative DNA polymerases." Biochem.Biophys.Res.Commun.249. 17-22 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ohtoshi,A: "Two recessive modes of growth inhibitionby exogenously introduced mutant genes: Analysis of mutant CDC28 and CDC7 genes" J.Biochem.Mol.Biol.Biophys.1. 253-263 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kim,J.M.: "Growth regulation ofthe expression of mouse cDNA and gene encoding a serine/threonine kinase related toS.cerevisiae CDC7 essential for" J.Biol.Chem.273. 23248-23257 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Jonstone,L.: "A Cdc7-Dbf4 kinase activity is conserved from yeast to human" Progress in Cell Cycle Research. Vol.4(in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takahashi,Y.: "Identification of three conserved regions in the DREF transcription factors from Drosophila melanogaster and Drosophila virilis." Nucleic Acids Res.27. 510-516 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Mizushina,Y.: "Studies on inhibotors of mammalian DNA polymerase a and b. Sulfolipids from a pteridophyte,Athyrium niponicum." Biochem.Pharmacol.55. 537-541 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Togashi,H.,: "4-Hydroxy-17-methylincisterol,and inhibitor of DNA polymerase-a activity and the growth of human cancer cell in vitro." Biochem.Pharmacol.56. 583-590 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka,H.,: "Fission yeast Cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S phase completion." Mol.Cell.Biol.,. 19. 1038-1048 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 片山 勉: "DNAポリメラーゼの新機能:染色体複製開始の負の制御" 蛋白質核酸酵素. (印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 滝澤温彦: "MCM/P1ファミリー蛋白質とDNA複製のライセンス化" 実験医学. 16巻8号. 61-66 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 白髭克彦: "複製開始領域の構成と染色体構築-出芽酵母の場合" 実験医学. 16. 31-40 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 白髭克彦: "酵母染色体のマルチレプリコン構造とチェックポイント制御 細胞周期研究の新しい展開(田矢,野島編) (印刷中)" 羊土社,

    • Related Report
      1998 Annual Research Report
  • [Publications] Yamashita,M.: "The efficiency and timing of initiation of DNA replication of multiple replicons of Saccharomyces cerevisiae chromosome VI." Genes to Cells. 2. 655-665 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okuno,Y.: "Identification of a predominant replication origin in fission yeast." Nucleic Acids Research.25. 530-536 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohtoshi,A.: "Two recessive modes of growth inhibition by exogenously introduced mutant genes : Analysis of mutant CDC28 and CDC7 genes in S. cerevisiae." J.Biochem.Mol.Biol.Biophys.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Masai,H.: "Frpo,a novel single-stranded DNA promoter for transcription and for primer RNA synthesis of DNA replication." Cell. 89. 897-907 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sato,N.: "Human and Xenopus cDNAs encodingbudding yeast Cdc7-related kinases : in vitro phosphrylation of MCM subunits by a putative human Cdc7." EMBO J.16. 4340-4351 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohtoshi A.: "Analysis of S.cerevisiae Cdc7 kinase point mutants : Dominant negative inhibition of DNA replication by overexpression of kinase-negative Cdc7 proteins." Mol.Gen.Genet.254. 562-570 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ryu,J.-R.: "Transcriptional regulation of the Drosophila raf protooncogene by the DNA replication-related element (DRE)/DRE-binding factor (DREF) system." Nucleic Acids Res.25 4. 794-799 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mohamed,M.M.: "In vitro DNA synthesis error specificity : A single amino-acid replacement in rat DNA polymerase b." Environ.Mutagen Res.19 (1). 7-15 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hayashi,Y.: "Identification of CFDD and role its binding site in regulation of the proliferating cell nuclear antigen gene promoter." J.Biol.Chem.272 (36). 23848-22858 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takahashi,Y.: "Involvement of the DNA replication-related element (DRE) and DRE-binding factor (DREF) in transcriptional regulation of the Bombyxmori PCNA gene." J.Biochem.122 (6). 1215-1223 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kuge,M.: "Use of a fusion protein to obtain crystal suitable for X-ray analysis : Crystallization of a GST-fused protein containing the DNA-binding domain of DREF." Protein Sci.6. 1783-1786 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaguchi,M.: "Distinct roles of E2F recognition sites as positive and negative elements in regulation of DNA polymerase a 180 kDa catalytic subunit gene promoter." Nucleic Acids Res.25 (19). 3847-3854 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoyagi,N.: "DNA polymerase e from Drosophila melanogaster." Biochem.Biophys.Res.Commun.230 (2). 297-301 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kubota,Y.: "Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs." EMBO J.16. 3320-3331 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Thommes,P.: "The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides." EMBO J.16. 3312-3319 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kubota,Y.: "Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs." EMBO J.in press.

    • Related Report
      1996 Annual Research Report
  • [Publications] Thommes,P.: "The RLF-M component of the replication licensing system forms complexes containing all six MCM/Pl polypeptides." EMBO J.in press.

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi,M.: "Roles of muliple promoter elements of the proliferating cell unclear antigen gene during Drosophila development." Genes to Cells. 1. 47-58 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hirose,F.: "Isolation and characterization of cDNA for DREF,a promoter-activating factor for Drosophila DNA replication-related genes.," J.Biol.Chem.271. 3930-3937 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takahashi,Y.: "DNA replication-related elements cooperate to enhance promoter activity of the Drosophila DNA polymerase a 73-kDa subunit gene." J.Biol.Chem.271. 14541-14547 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohno,K.: "Transcriptional regulation of the Drosophila CycA gene by the DNA replication-related element(DRE) and DRE-binding factor(DREF)." Nucleic Acids Res.24. 3942-3946 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ryu,J.-R.: "Trancriptional regulation of the Drosophila raf protooncogene by the DNA replication-related element (DRE)/DRE-binding factor(DREF) system." Nucleic Acids Res.,in press.

    • Related Report
      1996 Annual Research Report
  • [Publications] Masai,H.: "Mecahnisms of primer RNA synthesis and D-loop/R-loop-dependent DNA replication of Escherichia coli" Biochimie in press.

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohotoshi,A.: "Analyses of S.cerevisiae Cdc7 kinase point mutants:dominant negative inhibition of DNA replication by overexpression of non-functional Cdc7 protins" Mol.Gen.Genet.in press.

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohotoshi,A.: "Genetic interactions between CDC7 and CDC28:growh inhibition of cdc28-1N by cdc7 point mutants" Genes to Cells. 1. 895-904 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ikeda,M.: "A rapid and convenient method for screening of DNA binding and protein-interacting molecules by the use of a GST-fusion protein library" Gene. 181. 167-171 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ikeda,M.: "CTBP-1/RBP-1 of Saccharomyces cerevisiae protein which binds to T-rich single-stranded DNA containing the 11 base-pair core seqeunce of autonomously replicating seqence,is apolydeoxypyrimidine binding protein" Eur.J.Biochem.238. 38-47 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masai,H.: "DnaA-and PriA-dependent primosomes:two distinct replication complexes for replication of Escherichia coli chromosome" Frontiers in Bioscience. 1. 49-59 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Obuse,C.: "A replication-enhancing element with transcriptional silencing activity in autonomously replicating human chromosomal fragments" Mol.Biol.Cell. 7. 43-55 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okuno,Y.: "Identification of a predominant replication origin in fission yeast" Nucleic Acids Res.25. 530-536 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2019-02-15  

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