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Molecular approach for malaria control

Research Project

Project/Area Number 08281102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionThe University of Tokyo

Principal Investigator

KITA Kiyoshi (1999-2000)  東京大学, 大学院・医学系研究科, 教授 (90134444)

小島 荘明 (小島 莊明) (1996-1999)  東京大学, 医科学研究所, 教授 (00009622)

Co-Investigator(Kenkyū-buntansha) TANABE Kazuyuki  大阪工業大学, 工学部, 教授 (40047410)
HORII Toshihiro  大阪大学, 微生物病研究所, 教授 (80142305)
WATAYA Yusuke  岡山大学, 大学院・薬学研究科, 教授 (90127598)
脇 誠治  群馬県立医療短期大学, 教授 (10056286)
KOJIMA Somei  Institute of Medical Sciences, Professor, 医科学研究所, 教授 (00009622)
AIKAWA Masamichi  Tokai University, Research Institute of Medical Sciences, Professor, 総合科学技術研究所, 教授 (90271593)
北 潔  東京大学, 大学院・医学系研究科, 教授 (90134444)
Project Period (FY) 1996 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥46,500,000 (Direct Cost: ¥46,500,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 1998: ¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 1997: ¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 1996: ¥9,500,000 (Direct Cost: ¥9,500,000)
KeywordsMalaria / Tropical infectious diseases / Parasitic adaptation / vaccine / drug developmen / immunoresponse / animal model / vector / 免疫応答 / 実験動物モデル / ベクター
Research Abstract

本研究はマラリア原虫の寄生適応現象を分子レベルで研究する事により、新しいマラリア制圧学の構築ともいうべき新領域を確立する事を目的としてきた。この具体的な目標として、新しい化学療法、感染および発症に対する予防ワクチンの開発を設定し、安易な臨床応用ではない徹底的な基礎研究に立脚した研究計画が特徴となっている。
今年度は本特定領域研究成果の取りまとめの年であり、その実施にあたって各計画班間、班員間の共同研究、情報交換など有機的ネットワークの確立、研究成果の評価、並びに国際交流などの重要事項を総括してきた。なかでも抗マラリア剤のスクリーニングシステムは大きな反響があり、本年度も多数の新規化合物や天然物の情報が寄せられた。また研究領域内、さらに一般への広報活動の一環として「マラリアレター」および「マラリアホームページ」を行って来たが、他領域の研究者からの関心が強く内容の充実と規模を拡大て対応した。また、本研究ではこれまでの科学的な研究成果の取りまとめを行って研究報告書として公表するとともに、広く一般の研究者や関連分野の人々の理解を目的として公開班会議を行った。これらに加えて、先に述べたスクリーニングシステムや広報活動を本特定研究終了後も如何にして定着させ、持続させて行くかについての調査研究を行った。実際にはこれまでの総括班のメンバーの中から代表者、計画班班長、広報担当、事務担当などを選抜し、実質的な討議を行い、さらに本特定研究に参加して来た研究者の協力を得て次世代のマラリア研究の基盤を確立する事ができた。

Report

(6 results)
  • 2000 Annual Research Report
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Takeo,S.: "Succinate dehydrogenase in Plasmodium falciparum mitochondria : Molecular characterization of the SDHA and SDHB genes for the catalytic subunits, the flavoprotein (Fp) and iron-sulfur (Ip) subunits."Mol.Biochem.Parasitol.. 107. 191-205 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kita,K.: "Parasite mitochondria as a target for chemotherapy"J.Health Sci.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 田辺和裄: "マラリア学ラボマニュアル"菜根出版. 290 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takeo, S.: "Succinate dehydrogenase in Plasmodium falciparum mitochondria : Molecular characterization of the SDHA and SDHB genes for the catalytic subunits, the flavoprotein(Fp) and iron-sulfur (Ip) subunits"Mol. Biochem. Parasitol.. 107. 191-205 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takashima, E.: "Isolation of mitochondria from Plasmodium falciparum showing dihydroorotate dependent respiration"Parasitol. Int.. 50. 273-278 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okech, B.A.: "Natural human IgG subclass responses to Plasmodium falcoarum serine repeat antigen (SERA) in Uganda"Am. J. Trop. Med. Hyg.. 65. 912-917 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Itoh, T.: "Synthesis of novel ferrocenyl sugars and their antimalarial activities"Bioorg. Med. Chem. Lett.. 10. 1657-1659 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Putaporntip, C.: "Intragenic recombination in the 3' portion of the merozoite surface protein 1 gene of Plasmodium vivax"Mol. Biochem. Parasitol.. 109. 111-120 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Perlmann, P.: "Contrasting functions of IgG and IgE antimalarial antibodies in uncomplicated and severe Plasmodium falciparum malaria"Am. J. Trop. Med. Hyg.. 62. 373-377 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takeo, S.: "Succinate dehydrogenase in Plasmodium falciparum mitochondria : Molecular characterization of the SDHA and SDHB genes for the catalytic subunits, the flavoprotein (Fp) and iron-sulfur (Ip) subunits"Mol. Biochem. Parasitol.. 107. 191-205 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takashima, E.: "Isolation of mitochondria from Plasmodium falciparum showing dihydroorotate dependent respiration"Parasitol Int.. 50. 273-278 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Okech, B.A.: "Natural hunan IgG subclass responses to Plasmodium falciparum serine repeat autigen (SERA) in Uganda"Am. J. Trop. Med. Hyg.. 65. 912-917 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Itoh, T.: "Synthesis of novel ferrocenyl sugur and their antimalarial activities"Bioorg. Med. Cbem. Lett.. 10. 1657-1659 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Putaporntip, C.: "Intragenic recombination in the 3' portion of the merozoite surface protein 1 gene of Plasmodium vivax"Mol. Biochem. Parasitol.. 109. 111-120 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Perlmann, P.: "Contrasting functions of IgG and IgE antimalarial antibodies in uncomplicated and severe Plasmodium falclparum malaria"Am. J. Trop. Med. Hyg.. 62. 373-377 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Feng, C.: "An alternate pathway for type 1 T cell differentiation"Int. Immunol.. 11. 1185-1194 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Pang, X-L.: "Antibodies specific to N-terminal domain of Plasmodium falciparum serine repeat antigen (SERA) agglutinate merozoites and schizonts"Infect. Immun.. 67. 1821-1827 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Belperron, A. A.: "Immune responses induced by gene gun or intramuscular infection of DNA vaccines that express immunogenic regions of the serine repeat antigen (SERA) from Plasmodium falciparum and worldwide collected wild isolate"Infect. Immun.. 67. 5163-5169 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kim, H. S.: "Synthesis and antimalarial activity of cyclic peroxides, 1,2,4,5,7-pentoxocanes and 1,2,4,5-tetroxanes"J. Med. Chem.. 42. 2604-2609 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tanabe, K.: "Selection and genetic drift of polymorpisms within the merozoite surface protein-1 gene of Plasmodium falciparum"Gene (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Takeo, S.: "Succinate dehydrogenase in Plasmodium falciparum mitochondria: Molecular characterization of the SDHA and SDHB genes for the catalytic subunits, the flavoprotein (Fp) and iron -sulfur (Ip) subunits"Mol. Biochem Parasitol. In press.

    • Related Report
      1999 Annual Research Report
  • [Publications] Kojima,S: "Immunoregulation and parasitic infections." FEMS Immunol.Med.Microbiol.18. 319-324 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Pang X.-L.and Horii,T.: "Complement-mediated killing of Plasmodium falciparum erythrocytic schizont with antibodies to the recombinant Serine Repeat Antigen(SERA)" Vaccine. 16. 1299-1305 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takaya,Y.,Kurumada,K.,Takeuji,Y.,Kim,H.S.,Shibata,Y.,Ikemoto,N.,Wataya,Y.and Oshima,Y.: "Novel antimalarial guaiane-type sesquiterpenoids from Nardostachys chinensis roots." Tetrahedron Lett.39. 1361-1364 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Jongwutiwes,S.,Putaporntip,C.,Kanbara,H.and Tanabe,K.: "Variation in the thrombospondin related adhesive protein (TRAP) gene of Plasmodium falciparum from Thai field isolates." Mol.Biochem.Parasitol.92. 349-353 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Williamson,K.C.,Fujioka,H.,Aikawa,M.and Kaslow,D.C.: "Stage-specific processing of Pfs230,a Plasmodium falciparum transmission-blocking vaccine candidate." Mol.Biochem.Parasitol.(In Press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Yabu,Y.,Minagawa,N.,Kita,K.,Nagai,K,Honma,M.,Sakajo,S.,Koike,T.,Ohta,N.,Yoshimoto,A.: "Oral and intraperitoneal treatment of Trypanosoma brucei brucei with a combination of ascofuranone and glycerol in mice" Parasitology International. 47. 131-137 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 小島,莊明: "Species-specific diagnosis of human malaria by the polymerase chain reaction" Int.Med.Res.J.Vol.1,No.1. 69-72 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 堀井俊宏: "Sequence diversity in the amino-terminal 47 kDa fragment of the Plasmodium falciparum serine repeat antigen(SERA)" Mol.Biochem.Parasitol.Vol.86. 249-254 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 綿矢有佑: "Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene" Parasitol.Int.Vol.46. 91-95 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 田辺和裄: "Plasmodium falciparum:allelic variation in the merozoite surface protein 1 gene in wild isolates from southem Viertnam" Exp.Parasitol. Vol.86. 45-57 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 相川正道: "Molecular characterization and localization of Plasmodium falciparum HSP 60" Mol.Biochem.Parasitol.Vol.88. 95-104 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 北 潔: "Vanations in the C-terminal repeats of the knob-associated histidine-rich protein of Plasmodium falciparum" Biochim.Biophys.Acta. Vol.1360. 105-108 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 小島莊明: "Cytochrome c oxidase III(COIII)gene of Plasmodium vivax:complete coding sequence and its expression in the erythrocytic stages of the parasite" Jpn.J.Parasitol.Vol.45,No.5. 373-383 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 堀井俊宏: "Recombinant SERA proteins of Plasmodium falciparum induce growth inhibitory anti-sera against asexual stage of malaria parasite" Vaccine. Vol.14. 1069-1076 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 綿矢有佑: "Sequence variation in the 18S rRNA gene,a target for PCR-based mararia diagnosis,in Plasmodium ovale from Southern Vietnam" J.Clin.Microbiol.Vol.34. 2287-2289 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田辺和裄: "Plasmodium falciparum:variation in block 4 of the precursor to the major merozoite surface proteins in natural populations" Exp.Parasitol.Vol.84. 92-95 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 相川正道: "A developmental dafect in Plasmodium falciparum male gametogenesis." J.Cell.Biol.Vol.135,No.1. 269-278 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 北 潔: "An antibiotic,ascofuranone,specifically inhibits respiration and in vitro growth of long slender bloodstream forms of Trypanosoma brucei brucei" Mol.Biochem.Parasitol.Vol.81. 127-136 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2017-05-19  

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