Human Genome Analysis

• Project/Area Number: 08283108
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas (A)
• Allocation Type: Single-year Grants
• Research Institution: National Cancer Center Research Institute
• Principal Investigator: OHKI Misao National Cancer Ctr. Res. Inst., Cancer Genomics Div., Chief, 腫瘍ゲノム解析・情報研究部, 部長 (00158792)
• Co-Investigator(Kenkyū-buntansha): INOKO Hidetoshi Tokai Univ., School of Medicine, Prof., 医学部, 教授 (10101932) ; SHIMIZU Nobuyoshi Keio Univ., School of Medicine, Prof., 医学部, 教授 (50162706) ; SAKAKI Yoshiyuki The Institute of Meddical Science, The Univ. of Tokyo, Professor, 医科学研究所, 教授 (10112327) ; NIIKAWA Norio Nagasaki Uuiv., School of Medicine, Prof., 医学部, 教授 (00111170) ; ABE Tatsuo Kyoto Prefectural Univ., School of Medicine, Prof., 医学部, 教授 (60079746) ; 辻 省次 新潟大学, 脳研究所, 教授 (70150612) ; 林 健志 九州大学, 遺伝子情報実験施設, 教授 (00019671) ; 戸田 達史 東京大学, 医科学研究所, 助教授 (30262025)
• Project Period (FY): 1996 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥951,900,000 (Direct Cost: ¥951,900,000); Fiscal Year 2000: ¥174,600,000 (Direct Cost: ¥174,600,000); Fiscal Year 1999: ¥182,400,000 (Direct Cost: ¥182,400,000); Fiscal Year 1998: ¥182,200,000 (Direct Cost: ¥182,200,000); Fiscal Year 1997: ¥204,700,000 (Direct Cost: ¥204,700,000); Fiscal Year 1996: ¥208,000,000 (Direct Cost: ¥208,000,000)
• Keywords: Chromosome 21 / Chromosome 22 / Chromosome 11 / Whole Sequence / Hereditary defness DFNB8 / B10 / Early onset ataxia / Endometrial Cancer / SCAN method / ドラフトシーケンス / 遺伝性聾DFNB8・B10 / Sotos症候群 / 11q23 / ヒト21番染色体 / 進行性骨異形症 / TGFβ1遺伝子 / Bardet-Biedl症候群 / がん抑制遺伝子 / SKY法 / ヒト11q22-23領域 / S番染色体特異的BACクローン / シークエンス解析 / HLAクラス1遺伝子群 / PLACE-SSCP / SKY法の改良 / Transcriptional sequencing / 11q13領域 / シークエンス地図 / MHCクラスI領域 / 自己免疫疾患APECED / 福山型先天性ジストロフィー / 変異検出法 / 高速大規模プラスミド調整機 / 11番染色体物理地図 / ダウン症必須領域 / 免疫グロブリン遺伝子 / HLA遺伝子群 / SCA2遺伝子 / 福山型筋ジストロフィー / 改良型SSCP法

Research Abstract

A Complete Notl restriction map of the long arm of chromosome 11 which spans more than 80 Mb long was constructed. Some members supported by this grant contributed largely to determining the sequence and gene catalog of the long arms of chromosome 21 and 22 as members of international consortia. One of the above members determined draft sequences of chromosome 11 and 18 totaling about 180 Mb. The other sequencing team member identified that TMPRSS3 encoding membrane spanning protease is the hereditary defness gene DFNB8/B10. The gene loci for early onset ataxia with hypoalbuminemia and familial essential myoclonus and epilepsy were determined by genetic analysis and we succeeded in identifying regions to show strong linkage disequilibrium. Molecular cloning of the breakpoint region of t(5;8)(q35;q24.1)found in a Sotos syndrome patient led to the identification of the Sotos1 gene which might be causative for Sotos syndrome. Six megabase region of 11q23 has been suggested to contain tumo r suppressor gene(s) for breast, lung, endometrial cancers and neuroblastoma by previous LOH analyzes. Construction of transcription map identified 59 genes within the region. Somatic mutations were found in one of these in lung and endometrial cancers suggesting that the relevant gene is a tumor suppressor. One megabase region of 6q21 and 100 kb region of 10q25-q26 are candidate regions to show the presence of tumor suppressor genes for pancreatic cancer, respectively. Sequence analysis of the region was recently completed. Gene expression profiles of 7,000 genes in response to gamma-radiation was analyzed by a SAGE method and data-base was constructed. Characterization of human artificial chromosomes revealed that alfoid DNA larger than 70 kb allows to form chromatin structure specific for centromere. Computer-assisted technology system to assemble raw sequence data from full length cDNA, to find sequence errors, and to change to more reliable sequence of proteins was established. Spectral color banding which distinguishes between Guimsa stained bands by different colors was developed. This allows to identify breakpoints of chromosome translocations at the level of G-band which are not resolved by conventional SKY methods. PLACE SSCP method to use multichannel capillary electrophoresis was established by developing suitable matrix material and computer software.

Research Products

• [Publications] Hattori, M.: "The DNA sequence of human chromosome 21"Nature. 405. 311-319 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] International Human Genome Sequencing Consortium: "Initial sequencing and analysis of human genome"Nature. 409. 860-921 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Scott, H.: "Insertion of β-satellite repeats identifies a transmembrane protease causing both congenital and childhood onset autosomal recessive deafness"Nature Genet.. 27. 59-63 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kinoshita, A.: "Domain specific mutations in the human transforming growth factor beta 1 gene (TGFB1) result in Camurati-Englemann disease"Nature Genet.. 26. 19-20 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Thc RIKEN Genome Exploration Research Group Phase II Team, the FANTOM Consortium: "Functional annotation of 21,076 sequenced mouse cDNAs prepared from full-length enriched libraries"Nature. 409. 685-690 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kakazu, N.: "Development of spectral color banding in cytogenetic analysis"Lancet. 357. 529-530 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, M: "The DNA sequence of human chromosom 21"Nature. 405. 311-319 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] International Human Genome Sequencing Consortium: "Initial sequencing and analysis of human genome"Nature. 409. 860-921 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Scott, H.: "Insertion of ヲツ-satellite repeats identifies a transmembrane protease causing both congenital and childhood onset autosomal recessive deafness"Nature Genet.. 27. 59-63 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kinoshita, A: "Domain specific mutations in the human transforming growth factor beta 1 gene (TCFB1) result in Camurati-Englemann disease."Nature Genet.. 26. 19-20 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium: "Functional annotation of 21,076 sequenced mouse cDNAs prepared from full-length enriched libraries"Nature. 409. 685-690 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kakazu, N.: "Development of spectral colar banding in cytogenetic analysis"Lancet. 357. 529-530 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Morohoshi,F.: "Structure and expression pattern of a human MTG8/ETO family gene, MTGR1."Gene. 241. 287-295 (2000)
• [Publications] Shimizu,K.: "AML1-MTG8 leukemic protein induces the expression of granulocyte colony-stimulating factor (G-CSF) receptor through the up-regulation of CCAAT/enhancer binding protein epsilon."Blood. 96. 288-296 (2000)
• [Publications] Shimada,H.: "Analysis of genes under the Downstream control of the t(8;21) fusion protein AML1-MTG8 : Overexpression of the TIS11b (ERF-1, cMG1) gene induces myeloid cell proliferation in response to G-CSF."Blood. 96. 655-663 (2000)
• [Publications] Ohira,M.: "Identification and chracterization of a 500-kb homozygously deleted region at 1p36.2-p36.3 in a neulobrastoma cell line."Oncogene. 19. 4302-4307 (2000)
• [Publications] Kitabayashi,I.: "Fusion of MOZ and p300 histone acetyltransferases in acute monocytic leukemia with a t(8:22)(p11;q13) chromosome translocation."Leukemia. (in press).
• [Publications] Suzuki,T.: "Molecular cloning of a novel apoptosis-related gene, human Nap1(NCKAP1), and its possible relation to Alzheimer disease."Genomics. 63. 246-254 (2000)
• [Publications] Ito,T.: "Toward a protein-protein interaction map of the budding yeast : A comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins."Proc.Nail.Acad.Sci.,U.S.A.. 97. 1143-1147 (2000)
• [Publications] Iijima,Y.: "A new ETV/TEL partner gene, ARC (ABL-related gene or ABL2), identified in an AML-M3 cell line with a t(1;12)(q25;13) translocation."Blood. 15. 2126-2131 (2000)
• [Publications] Hida,A.: "The human and mouse Period1 genes : Five well-conserved e-boxes additively contribute to the enhancement of mPer1 transcription."Genomics. 65. 224-233 (2000)
• [Publications] Yamazaki,S.: "Resetting central and peripheral circadian oscillators in transgenic rats."Science. 288. 682-685 (2000)
• [Publications] Hattori,M.: "The DNA sequence of human chromosome 21."Nature. 405. 311-319 (2000)
• [Publications] Togashi,T.: "A novel gene, DSCR5, from the distal down syndrome critical region on chromosome 21q22.2."DNA Res.. 7. 207-212 (2000)
• [Publications] Yatsuki,H.: "Sequence-based structural features between Kvlqt1 and Tapa1 on mouse chromosome 7F4/F5 coneponding to the Beckwith-Wiedeman n syndrome region on human 11p15.5 : long stretches of unusually well conserved intronic sequences of Kvlqt1 between mouse and human."DNA Res.. 7. 195-206 (2000)
• [Publications] Kubota,H.: "G1 domain-mediated association of the eukaryotic initiation factor 2 α kinase GCN2 with its activator GCN1 is required for general amino acid control in budding yeast."J.Biol.Chem.. 275. 20243-20246 (2000)
• [Publications] Shigenobu,S.: "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."Nature. 407. 81-86 (2000)
• [Publications] Mizushima,K.: "A novel G-protein coupled receptor gene expressed in striatum."Genomics. 69. 314-321 (2000)
• [Publications] Inaternational Human Genome Sequencing Consortium: "Initial sequencing and analysis of the human genome."Nature. 409. 860-921 (2001)
• [Publications] Shiina,T.: "The beta 1,3-galactosyltransferase-4 (B3GALT4) gene is located in the centromeric segment of the human MHC class II region."Imuunogenetics. 51. 75-78 (2000)
• [Publications] Gao,P.S.: "Variants of NOS1, NOS2 and NOS3 genes in Asthmatics."Biochem.Biophys.Res.Commun.. 267. 761-763 (2000)
• [Publications] Teraoka,Y.: "Genetic polymorphisms in the cell growth regulated gene. SC1 telomeric of the HLA-C gene and lack of association with psoriasis vulgaris."Tissue Antigens. 55. 206-211 (2000)
• [Publications] Dai,K.Z.: "The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd) is localized centromeric to the CD1 gene clusrter on human chromosome 1."Immunogenetics. 51. 179-185 (2000)
• [Publications] Keicho,N.: "Fine localization of a major disease-susceptibility locus for diffuse panbronchiolitis."Am.J.Hum Genet.. 66. 501-507 (2000)
• [Publications] Watanabe,Y.: "Replication timing of the human X-inactivation center (XIC) region : correlation with chromosome bands."Gene. 252. 163-172 (2000)
• [Publications] Naruse,T.K.: "HLA-DQ1 ^*0601 is primarily associated with the susceptibility to cardiac sarcoidosis."Tissue Antigens. 56. 52-57 (2000)
• [Publications] Mizuki,N.: "Localization of the pathogenic gene of Behcet's disease by microsatellite analysis of three different populations."Invest.Ophthalmol.Vis.Sci.. 41. 3702-3708 (2000)
• [Publications] Matsuzaka,Y.: "New polymorphic microsatellite markers in the human MHC class II region."Tissue Antigens. 56. 492-500 (2000)
• [Publications] Saito,S.: "Allele frequencies and haplotypic associations defined by allelic DNA typing at HLA class 1 and Class II loci in the Japanese population."Tissue Antigens. 56. 522-529 (2000)
• [Publications] Ota,M.: "A second susceptibility gene for developing rheumatoid arthritis in the human MHC is localized within a 70 kb interval telomeric of the TNF genes in the HLA class III region."Genomics. (in press).
• [Publications] Sugata,N.: "Human CENP-H multimers colocalize with CENP-A and CENP-C at active centromere-kinetochore complexes."Human Mol.Genet.. 9. 2919-2926 (2000)
• [Publications] Shibasaki,Y.: "Linkage of autosomal recesssive hereditary spastic paraplegia with mental impairment and thin corpus callosum to chromosome 15q13-15."Ann.Neurol.. 48. 108-112 (2000)
• [Publications] Takano,H.: "Mutational analysis of X-linked adrenoleukodystrophy gene."Cell.Biohem.Biophys.. 32. 177-185 (2000)
• [Publications] Shimohata,T.: "Expanded polyglutamine stretches associated with CAG repeat diseases interact with TAFII130, interfering with CREB-dependent transcription."Nature Genet.. 26. 29-35 (2000)
• [Publications] Ohara,K.: "A CAG trinucleotide repeat expansion and familial schizophrenia."Psychiat.Res.. 94. 257-262 (2000)
• [Publications] Aapola,U.: "Isolation and initial characterization of a novel zinc finger gone, DNMT3L on 21q22.3, related to cytosine-5-methyltransferase 3 gene family."Genomics. 65. 293-298 (2000)
• [Publications] Shibuya,K.: "Isolation of two novel genes, DSCR5 and DSCR6, from Down syndrome critical region on human chromosome 21 q22.2."Biochem.Biophys.Res.Commun.. 271. 693-698 (2000)
• [Publications] The chromosome 21 mapping and sequencing consortium: "The DNA sequence of human chromosome 21."Nature. 405. 311-319 (2000)
• [Publications] Pitkanen,J.: "The autoimmune regulator protein has transcriptional transactivating properties and interacts with the common coactivator CREB-binding protein."J.Biol.Chem.. 275. 16802-16809 (2000)
• [Publications] Berry,A.: "Refined localization of autosomal recessive non-syndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure and exclusion of six known genes in the region."Genomics. 68. 22-29 (2000)
• [Publications] Guipponi,M.: "C2lorf5, a novel human chromosome 21 gene, has a Caenorhabditis elegans ortholog (pad-1) required for embryonic patterning."Genomics. 68. 30-40 (2000)
• [Publications] Michaud,J.: "Isolation and characterization of a human chromosome 21q22.3 gene (WDR4) and its mouse homologue that code for a WD-repeat protein."Genomics. 68. 71-79 (2000)
• [Publications] Bartoloni,L.: "Cloning and characrterization of a putative human glycerol 3-phosphate permease gene (SLC37A1 or G3PP) on mutation analysis in 2 candidate phenotypes, DFNB10 and a glycerol kinase deficiency."Genomics. 70. 190-200 (2000)
• [Publications] Scott,H.: "Insertion of β-satellite repeats identifies a transmembrane protease causing both congenital and childhood onset autosomal recessive deafness."Nature Genet.. 27. 59-63 (2001)
• [Publications] Jong,Y.J.: "Genetic heterogeneity in three Chinese children with Fukuyama congenital muscular dystrophy."Neuromuscul Disord. 10. 108-112 (2000)
• [Publications] Futaki,M.: "The IVS4+4 A-T mutation of the Fanconi anemia gene FANCC is not associated with a severe phenotype in Japanese patients."Blood. 95. 1493-1498 (2000)
• [Publications] Saito,K.: "Haplotype-phenotype correlation in Fukuyama congenital muscular dystrophy."Am.J.Med.Genet.. 92. 184-190 (2000)
• [Publications] Chadani,Y.: "Walker-Warburg syndrome is genetically distinct from Fukuyama type congenital muscular dystrophy."J.Nuerol Sci.. 177. 150-153 (2000)
• [Publications] Sasaki,J.: "Neuronal expression of the fukutin gene."Hum.Mol.Genet.. 9. 3083-3090 (2000)
• [Publications] Kobayashi,K.: "Structural organization, complete genomic sequences, and mutational analyses of the Fukuyama-type congenital muscular dystrophy gene, fukutin."FEBS Lett.. 489. 192-196 (2001)
• [Publications] Ghadamii,M.: "Genetic mapping of the Camurati-Engelmann disease locus to chromosome 19q13.2-q13.3."Am.J.Hum.Genet.. 66. 143-147 (2000)
• [Publications] Kinoshita,A.: "Domain specific mutations in the human transforming growth factor beta 1 gene (TGFB1) result in Camurati-Englemann disease."Nature Genet.. 26. 19-20 (2000)
• [Publications] Yamada,K.: "An autosomal dominant posterior polar cataract locus maps to human chromosome 20."Eur.J.Hum.Genet.. 8. 535-539 (2000)
• [Publications] Hayashida,S.: "Construction of a physical and transcript map flanking the imprinted MEST/PEG1 region at 7q32."Genomics. 66. 221-225 (2000)
• [Publications] Ghadami,M.: "Bardet-Biedl syndrome type 3 in an Iranian family : Clinical study and confirmation of disease localization."Am.J.Med.Genet.. 94. 433-437 (2000)
• [Publications] Ghadami,M.: "Comfirmation of genetic homogeneity of syndactyly type 1 in an Iranian family."Am.J.Med.Genet.. (in press).
• [Publications] Uemura,K.: "Deletion polymorphism of ACE gene is associated with higher blood pressure after hospitalization in normotensive subjects."Hypertens.Res.. 23. 201-205 (2000)
• [Publications] Uemura,K.: "Association of ACE 1/D polymorphism with cardiovascular risk factors."Hum.Genet.. 107. 239-242 (2000)
• [Publications] Kamino,K.: "Deficiency in mitochondrial aldehyde dehydrogenase increases the risk for late-onset Alzheimer's disease."Biochem.Biophys.Res.Commun.. 273. 192-196 (2000)
• [Publications] Hatanaka,Y.: "Low density lipoprotein receptor-related protein gene polymorphisms and risk for late-onset Alzheimer's disease in a Japanese population."Clin.Genet.. 58. 319-323 (2000)
• [Publications] Muroya,K.: "Sex-determining gene (s) on distal 9p : Clinical and molecular studies in six cases."J.Clin.Endocrinol.Metab.. 85. 3094-3100 (2000)
• [Publications] Seki,H.: "Mutation frequencies of EXT1 and EXT2 in 43 Japanese families with hereditary multiple exostoses."Am.J.Med.Genet.. 99. 59-62 (2001)
• [Publications] Kitamura,Y.: "Association of allelic loss on 1q, 4p, 7q, 9p, and 16q with postoperative death in papillary thyroid carcinoma."Clin.Cancer Res.. 6. 1819-1825 (2000)
• [Publications] Kurose,K.: "Novel gene fusion of COX6C at 8q22-23 to HMGIC at 12q 15 in a uterine leiomyoma."Genes.Chrom.Cancer. 27. 303-307 (2000)
• [Publications] Kitamura,Y.: "Allelotyping of anaplastic thyroid carcinoma : Frequent allelic losses on 1q,9p,11,17,19p, and 22q."Genes.Chrom.Cancer. 27. 244-251 (2000)
• [Publications] Yokota,T.: "Genomic organization and chromosomal mapping of ELKS, a gene rearranged in a papillary thyroid carcinoma."J.Hum.Genet.. 45. 6-11 (2000)
• [Publications] Bando,K.: "Identification of a 1-Mb common region at 16q24.1-24.2 deleted in hepatocellular carcinoma."Genes.Chrom.Cancer. 28. 38-44 (2000)
• [Publications] Kurose,K.: "Three aberrent splicing variants of the HMGIC gene transcribed in uterine leiomyomas."Genes.Chrom.Cancer. 30. 212-217 (2001)
• [Publications] Akao,Y.: "Molecular analysis of the rearranged genome and chimeric mRNAs caused by the t(6;11)(q27;q23) chromosome translocation involving MLL in an infant acute monocytic leukemia."Genes.Chrom.Cancer. 27. 412-417 (2000)
• [Publications] Saitou,M.: "Identification of the TCL6 genes within the beakpoint cluster region on chromosome 14q32 in T-cell leukemia."Oncogene. 19. 2796-2802 (2000)
• [Publications] Fushimi,H.: "Genetic heterogeneity of ribosomal RNA gene and matK gene in Panax notoginseng."Planta.Medica.. 66. 659-661 (2000)
• [Publications] Suga,M.: "Cryopreservation of competent intact yeast cells for efficient electoroporation."Yeast. 16. 889-896 (2000)
• [Publications] Makino,N.: "Isolation and chracterization of the human gene homologous to the Drosophila headcase (hdc) gene in chromosome bands 6q23-q24, a region of common deletion in human pancreatic cancer."DNA Seq.. (in press).
• [Publications] Youssecf,E.M.: "Human BAC contig covering the deleted region in pancreatic cancer at 12q21."DNA seq.. (in press).
• [Publications] Yatsuoka,T.: "Association of poor prognosis with loss of 12q,7p, and 18q, and concordant loss of 6q/17p and 12q/18q in human pancreatic ductal adenocarcinoma."Am.J.Gastroenterol.. 95. 2080-2085 (2000)
• [Publications] Osoegewa,K.: "Bacterial artificial chromosome libraries for mouse sequencing and functional analysis."Genome.Res.. 10. 116-128 (2000)
• [Publications] Akiyoshi,S.: "A genetic linkage map of the MSM Japanese wild mouse strain with restriction landmark genomic scanning (RLGS)."Mammal Genome.. 11. 356-359 (2000)
• [Publications] Okazaki,Y.: "Normalization and subtraction of cap-trapper selected cDNAs to prepare full-length cDNA libraries for high-rate new gene discovery."Genome.Res.. 10. 1617-1630 (2000)
• [Publications] Shibata,K.: "RIKEN integrated sequence analysis system (RISA system)-384-format sequencing pipeline with 384 multi-capillary sequencer."Genome.Res.. 10. 1757-1771 (2000)
• [Publications] Sugahara,Y.: "Comparative evaluation of 5' -end-sequence quality and other chracteristics of clones in CAP trapper and other full-lengh-cDNA libraries."Gene. (in press).
• [Publications] Konno,H.: "Computer-based methods for a mouse full-length cDNA project : real-time sequence clustering for construction of a non-redundnt library."Genome.Res.. 11. 281-289 (2001)
• [Publications] Miki,R.: "Gene expression profiling using the 19K set of RIKEN full-length mouse cDNA microarray."Proc.Natl.Acd.Sci.,U.S.A.. (in press).
• [Publications] The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium: "Functional annotation of 21,076 sequenced mouse cDNAs prepared from full-length enriched libraries."Nature. 409. 685-690 (2001)
• [Publications] International Human Genome Sequencing Consortium: "Initial sequencing and analysis of the human genome."Nature. 409. 860-921 (2001)
• [Publications] Park,H.S.: "Newly identified repeat sequences, deried from human chromosome 21q-ter, are also localized in the subtelomeric region of particular chromosomes and 2q13, and are conserved in the chimpanzee genome."FEBS Lett.. 475. 167-169 (2000)
• [Publications] Bruls,T.: "A clone map of human chromosome 14."Nature. 409. 947-948 (2001)
• [Publications] kakazu,N.: "Characterization of complex chromosomal abnormalities in B-cell lymphoma by a combined spectral karyotyping (SKY) analysis and fluorescene in situ hybridization (FISH) using a 14q telomere probe."Am.J.Hematol.. 65. 291-297 (2000)
• [Publications] Kuroda,J.: "Aggressive natural killer cell leukemia/lymphoma : a comprehensive cytogenetic study by spectral karyotyping."Ann.Hematol.. 79. 519-522 (2000)
• [Publications] Koide,K.: "An improved rapid procedure for fluorescence in situ hybridization that is applicable to intraoperative cancer cytodiagnosis."Cancer Lett.. 158. 165-169 (2000)
• [Publications] Minamiguchi,H.: "Interleukin 6 receptor expression by human cord blood- or peripheral blood-derived primitive haematopoietic progenitors implies acquisition of different functional properties."Br.J.Haematol.. 110. 327-338 (2000)
• [Publications] Okuda,T.: "Biological chrateristics of the leukemia-associated transcriptional factor AML1 disclosed by hematopoietic rescue of AML1-deficient embryonic stem cells by using a knock-in strategy."Mol.Cell.Biol.. 20. 319-328 (2000)
• [Publications] Kakazu,N.: "Development of spectral color banding in cytogenetic analysis."Lancet. 357. 529-530 (2001)
• [Publications] Horiuchi,T.: "Dominant expression of a novel splice variant of caspase-8 in human peripheral blood lymphocytes."Biochem.Biophys.Res.Commun.. 272. 877-881 (2000)
• [Publications] Kondo,H.: "Microsatellite genotyping of post-PCR fluorescently labeled markers."Biotechniques. 29. 868-872 (2000)
• [Publications] Sasaki,T.: "Precise estimation of allele frequencies of single-nucleotide polymorphisms by a quantitative SSCP analysis of pooled DNA."Am.J.Hum.Genet.. 68. 214-218 (2001)
• [Publications] Suzuki,A.: "Polar alteration of short tandem repeats (STRs) in mammlian cells."Mut.Res.. 474. 159-168 (2001)
• [Publications] Gomyo, H.: "A2-Md sequence-ready contig map and a novel immunoglobulin superfamily gene IGSF4 in the LOH region of chromosome 11q23.2"Genomics. 62. 139-146 (1999)
• [Publications] Ichikawa, H.: "Dual transforming activities of the FUS(TLS)-ERG leukemia fusion protein conferred by two N-teminal domains of FUS(TLS)"Mol. Cell. Blol.. 19. 7639-7650 (1999)
• [Publications] Morohoshi, F.: "Structure and expression pattern of a human MTG8/ETO family gene, MTGR1"Gene. 241. 287-295 (2000)
• [Publications] Narita, M.: "Consistent detection of CALM-AF10 chimaeric transcripts in haematological malignancies with t(10;11) (p13;q14) and indentification of novel transcripts"Brit. J. Haematol. 105. 928-937 (1999)
• [Publications] Tanaka, K.: "Detection of translocation 8;21 on interphase cells from acute myelocytic leukemia by fluorescence in situ hybridization and its clinical application"Cancer Genet. Cytogenet.. 113. 29-35 (1999)
• [Publications] Nishiyama, M.: "11p15 translocations involving the NUP98 gene in childhood therapy-related acute myeloid leukemia/Myelodysplastic syndrome"Gnes Chrom. Cancer. 26. 215-220 (1999)
• [Publications] 阿部 達生: "がんの細胞生物学"医学書院、東京. pp1-pp365 (2000)
• [Publications] Hayashi, K.: "SSCP analysis of point mutations by multi-color capillary electrophoresis"Humana Press, NJ, U.S.A.. ((in press))
• [Publications] Gamou,T.: "The partner gene of AMLI in t(16:21)myeloid malignencies is a novel member of the MTGS(ETO)family." Blood. 91. 4028-4037 (1998)
• [Publications] Murakami,Y.: "Localization of tumor suppressor activity important in non small cell lung carcinoma on chromosome11q." Proc.Natl.Acad.Sci.,USA. 95. S153-S158 (1998)
• [Publications] Morohoshi,F.: "Genomic structure of the human RBP56/HTAF_<11>68 and FUS/TLS genes." Gene. 221. 191-198 (1998)
• [Publications] Eguchi-Ishimae,M.: "Fluorescence in situ hybridization analysis on 12;21 translocation in Japanese childhood acute lymphoblastie leukemia." Jpn.J.Cancer Res.89. 783-788 (1998)
• [Publications] Shiraishi,M.: "The isolation of CpG islands from human chromosomal regions 11q13 and Xp22 by segregation of partlymelted molecules." Nuclec.Acids.Res.26. 5544-5550 (1998)
• [Publications] Kitabayashi,I.: "The AML1-MTG8 leukemic fusion protein forms a complex with a novel member of the MTG8(ETO/CDR)family." Mol.Cell.Biol.18. 846-858 (1998)
• [Publications] Sato,S.: "Splicing mutation of Presenilin-1 gene for early-onset familial Alzheimer's disease." Hum.Mutat.Supple.1. S91-S94 (1998)
• [Publications] Tsukahara,F.: "Molecular chracterization of the mouse mtprd gene,a homologue of human TPRD: unique gene expression suggesting its critical role in the pathophysiology of Down Syndrome." J.Biochem.123. 1055-1063 (1998)
• [Publications] Choi,D.K.: "Fluorescent differential display analysis of gene expression in apoptotic neuroblastoma cells." Gene. 223. 21-31 (1998)
• [Publications] Koike,N.: "Identification of the mammalian homologues of the Drosophila timeless gene.Timeless 1." FEBS Letter. 441. 427-431 (1998)
• [Publications] Hashida,H.: "Cloning and mapping of ZNF231,a novel brain-specific gene encoding neuronal double zine finger protein whose expression is enhanced in a neurodegenerative disorder,multiple system atrophy(MSA)." Genomics. 54. 50-58 (1998)
• [Publications] Shiina,T.: "Nucleotide sequencing analysis of the 146kb segment around the 1kBL and MICA genes at the centromenric end of the HLA class I region." Genomics. 47. 372-382 (1998)
• [Publications] Mizuki,N.: "Major histocompatibility complex class II alleles in an Uygur population in Northwest China." Tissue Antigens. 51. 287-292 (1998)
• [Publications] Naruse,T.K.: "Extended HLA haplotyopes in Japanese homozygous typing cells." Tissue Antigens. 51. 305-308 (1998)
• [Publications] Tamiya,G.: "Twenty-six new polymorphic microsatellites markers around the HAL-B.-C and -E loci in the human MHC class I region." Tissue Antigens. 51. 337-346 (1998)
• [Publications] Katsuyama,Y.: "Genetic relationships among Japanese,Northern Han,Hui,Uygur,Kazakh,Greek,Saudi Arabian and Italian populations based on allelic frequencies at four VNTR (DIS80,D4S43,COL2A1,D17S5)and one STR (ACTBP2)Loci." Human Heredity. 48. 126-137 (1998)
• [Publications] Taniguchi,Y.: "Nucleotide sequence of the Ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells." Genomics. 51. 114-123 (1998)
• [Publications] Ito,Y.: "Genomic structure of the spematid-specific Hsp70 homolog gene located in the class III region of the major histocompatibility complex of mouse and man." J.Biochem.124. 347-353 (1998)
• [Publications] Sazazuki,T.: "Imporance of HLA-class I allele matching for clinical outcome after unrelated hematopoietic stem cell transplanation." N.Engl.J.Med.339. 1177-1185 (1998)
• [Publications] Shiina,T.: "Physical mapping of 620 kb between the S and HLA-E genes in the midst of the human MHC class I region by construction of a BAC,PAC and cosmid contig." Immunogenetics. 48. 402-407 (1998)
• [Publications] Kawakami,S.: "Tetex3,related to drosophila polycomblike,is expressed in male germ cells and mapped to the mouse-complex." Mamm.Genome.9. 874-880 (1998)
• [Publications] Shigenari,A.: "Characterization of alkaline phosphatase genes expressed in seminoma by cDNA cloning." Cancer Res.58. 5079-5082 (1998)
• [Publications] Yabuki,K.: "Triplet repeat polymorphism in the MICA gene in HLA-B27 positive and negative Caucasian patients with anlkylosing spondylitis." Hum.Immunol.60. 83-86 (1998)
• [Publications] Ikeno,M.: "Construction of YAC based mammalian artificial chromosomes." Nature Biotech.16. 431-439 (1998)
• [Publications] Masumoto,H.: "Assay of centromere function using a human artificial chromosome." Chromosoma. 107. 406-416 (1998)
• [Publications] Igarashi,S.: "Suppression of aggregate formation and apoptosis by transglutaminase inhibitors in cells expressing truncated DRPLA protein with an expanded polyglutamine stretch." Nature Genet.18. 111-117 (1998)
• [Publications] Saito,M.: "Refinement of the gene locus for autosomal recessive juvenile perkinsonism (AR-JP)on chromosome 6q25.2-27 and idenification markers exhibiting linkage disequilibrium." J.Hum.Genet.43. 22-31 (1998)
• [Publications] Tanno,Y.: "Mitochondrial DNA polymorphisms in Japanese sporadic Alzheimer's disease." Neurobiol.Aging. 19. S47-S51 (1998)
• [Publications] Ikeuchi,T.: "A novel long and unstable CAG/CTG trinucleotide repeat on chromosome 17q." Genomics. 49. 321-326 (1998)
• [Publications] Takiyama,Y.: "Matemal anticipation in Machodo-Joseph disease (MJD):some matemal factors independent of the number of CAG repeat units may play a role in genetic anticipation i a Japanese MJD family." J.Nuerol.Sci.155. 141-145 (1998)
• [Publications] Hermann.T.: "Molecular cloning,structural organization,sequence,chromosomal assignment, and expression of the mouse alpha-N-acetylgalactosaminidase gene." Gene. 211. 205-214 (1998)
• [Publications] Onishi,Y.: "MERRF/MELAS overlap syndrome associated with 3243 tRNA^<Lew(UUR)> mutation of mitochondrial DNA." Neuropathology. 18. 321-327 (1998)
• [Publications] Takano,H.: "Close associations between prevalence rates of dominantly inherited spinocerebellar ataxias with CAG-repeat expansions and frequencies of large normal CAG alleles in Japanese and Caucasian populations." Am.J.Hum.Genet.63. 1060-1066 (1998)
• [Publications] Koide,T.: "Formation of granular cytoplasmic aggregates in cells expressing mutant Cu/Zn superoxide dismutase associated with familial amyotrophic lateral sclerosis." Neurosci.Lett.257. 29-32 (1998)
• [Publications] Okuizumi,K.: "Genetic polymorphism of the tau gene and neurodegenerative diseases with tau pathology among Japanese." Ann.Neurol.44. 707 (1998)
• [Publications] Sato,T: "Trangenic mice harboring a full-length human nutant DRPLA gene exhibit age-dependent intergenerational and somatic instabilities of CAG reptats comparable to those in DRPLA patients." Hum.Mol.Genet.8. 99-106 (1999)
• [Publications] Takano,H.: "Mutational analysis and genotype-phenotype comelation of 29 unrelated Japanese patients with X-linked adenoleukodystrophy(ALD)" Arch.Neurol.(in press).
• [Publications] Asakawa,S.: "High-fidelity digital hybridization screening." Genomics. 49. 209-217 (1998)
• [Publications] Takahashi,H.: "Mouse myocilin (Myoc)gene expression in ocular tissues." Biochem.Biophys.Res.Commun.248. 104-109 (1998)
• [Publications] Imamura,Y.: "Human retina-specific amine oxidase: genomic structure of the gene (AOC2)'altematively spliced variant,and mRNA expression in retina." Genomics. 51. 293-298 (1998)
• [Publications] Footz,T.K.: "The gene for death agonist BID maps to the region of human 22q11.2 duplicated in cat eye syndrome chromosomes and to mouse chromosome 6." Genomics. 51. 472-475 (1998)
• [Publications] Wang,J.: "Identification of two novel 5' noncoding exons in human MNB/DYRK gene and altematively spliced transcripts." Biochem.Biophys.Res.Commun.250. 704-710 (1998)
• [Publications] Nagamine,K.: "Molecular cloning of a novel putative Ca^<2+> channel protein (TRPC7)higyly expressed in brain." Genomics. 54. 124-131 (1998)
• [Publications] Sakamoto,O.: "Molecular analysis of new cases of Japanese patients of holocarboxylase synthetase deficiency." J.Inherit.Metab.Dis.21. 873-874 (1998)
• [Publications] Kobayashi,K.: "An ancient retrotransposal insertion causes Fukuyama-type congenital muscular dystrophy." Nature. 394. 388-392 (1998)
• [Publications] Kobayashi,K.: "Founder-haplotype analysis in Fukuyama-type congenital musclar dystrophy (FCMD)." Hum.Genel.103. 323-327 (1998)
• [Publications] Takai,Y.: "Prenatal diagnosis of Fukuyama-type congenital muscular dystrophy by microsatellite analysis." Hum.Reprod.13. 320-323 (1998)
• [Publications] Saito,K.: "Prenatal diagnosis in eight Fukuyama type congenital muscular dystrophy families by haplotype analysis using the new markers closest to the gene." Am.J.Med.Genet.26. 310-316 (1998)
• [Publications] Voit,T.: "Merosin positive congenital muscular dystorophy with transient brain dysmyelination and mental retardation." Neuromuscular Disord. (in press).
• [Publications] Toda,T.: "The Fukuyama-type congenital muscular dystrophy story." Neuromuscular Disord. (in press).
• [Publications] Tsujita,T.: "Genomic discordance between monozygotic twins discordant for schizophrenia." Am.J.Psychiat.155. 422-424 (1998)
• [Publications] Fujimoto,M.: "The gene for mesomelic dysplasia Kantaputra type is mapped to 2q24-q32." J.Hum.Genet.43. 32-36 (1998)
• [Publications] Yun,K.: "Promoter-specific insulin-like growth factor2 gene imprinting in human fetal liver and hepatoblastoma." J.Pathol.185. 91-98 (1998)
• [Publications] Maeda,T.: "Cloning and characterization of a novel human gene,TM4SF6,encoding a protein belonging to the transmembrane 4 superfamily,and mapped to Xq22." Genomics. 52. 240-242 (1998)
• [Publications] Nakano,M.: "Identification,chracterization and mapping of the human ZIS(zinc-finger,splicing)gene." Gene. (in press).
• [Publications] Ogaki,K.: "Mapping of a new target region of allelic loss to a 6-cM interval at 21q-21 in primary breast cancers." Genes.Chrom.Cancer.23. 244-247 (1998)
• [Publications] Kurose,K.: "Frequent allelic loss at 7p14-15 associated with aggressive histologic types of breast cancer." Jpn.J.Cancer Res.89. 533-538 (1998)
• [Publications] Minobe,K.: "Allelic loss on chromosome 9q is associated with lymph node metastasis of primary breast cancer." Jpn.J.Cancer.Res.89. 916-922 (1998)
• [Publications] Kurose,K.: "Somatic mutations of the PTEN/MMACI gene in fifteen Japanese endometnal cancers: Evidence for inactivation of both alleles." Jpn.J.Cancer Res.89. 842-848 (1998)
• [Publications] Iida.A.: "Mapping of a new target region of allelic loss to a 2-cM interval at 22q13.1 in primary breast cancer." Genes.Chrom.Cancer. 21. 108-112 (1998)
• [Publications] Tsukamoto,K.: "Allelic loss on chromosome lp is associated with progression and lymph node metastasis of primary breast carcinoma." Cancer. 82. 317-322 (1998)
• [Publications] Nakata,T.: "Identification of a new commonly deleted region within a 2-cM interval of 11p11 in breast cancers." Eur.J.Cancer. 34. 417-421 (1998)
• [Publications] Bando,K.: "Frequent allelic loss at 6q26-27 in breast cancer of the solid-tubular histologic type." Breast Cancer. 5. 127-130 (1998)
• [Publications] Keicho,N.: "Contribution of HLA genes to genetic predisposition in diffuse panbronchiolitis." Am.J.Respir.Crit.Care.Med.158. 846-850 (1998)
• [Publications] Kamigaki,M.: "Familial hypercholesterolemia with cholesterry ester transfer protein deficinecy." Intem.Med.37. 523-527 (1998)
• [Publications] Yamaki,E.: "Molecular genetic diagnosis of a family with hypercholesterolemia by a mismatched PCR-RFLP method for genotyping single base substitution of the LDL gene." Jpn.Heart.J.39. 681-686 (1998)
• [Publications] Hirayama,T.: "Five familial hypereholesterolemic kindreds in Japan with novel mutations of the LDL receptor gene." J.Hum.Genet.43. 250-254 (1998)
• [Publications] Emi,M.: "Multiplex mutation screening of the BRCAI gene in 1000 Japanese breast cancers." Jpn.J.Cancer Res.89. 12-16 (1998)
• [Publications] Yokota,T.: "Localization of a tumor suppressor gene associated with progression of human breast cancer within a 1-cM interval of 8p22-p23.1." Cancer. 83. 447-452 (1998)
• [Publications] Kurose,K.: "Hamartomatous polyposis of the digestive tract with mutation of the PTEN gene." Am.J.Hum.Genet.64. 308-310 (1999)
• [Publications] Nakata,T.: "Fusion of a novel gene,ELKS.to c-ret due to translocation t(10:12)(q11:p13)in a papillary thyroid carcinoma." Genes.Chrom.Cancer. 25(in press). (1999)
• [Publications] Ohgaki,K.: "Localization of tumor suppressor gene associated with distant metastasis of urinary bladder cancer to I-Mb interval on 8p22." Genes.Chrom.Cancer. 25(in press). (1999)
• [Publications] Fukino,K.: "Frequent ailelic loss at the TOC locus on 17q25.1 in primary breast cancers." Genes.Chrom.Cancer. 24. 345-350 (1999)
• [Publications] Ueda,T.: "Idenification of a 1-cM region of common deletion of 13q14 associated with metastasis of human prostate cancer." Genes.Chrom.Cancer. 24. 183-190 (1999)
• [Publications] Kitamura,Y.: "Ret/PTC3 is the most frequent form of gene rearrangement among papillary thyroid carcinomas in Japan." J.Hum.Genet.44. 96-102 (1999)
• [Publications] Yokota,T.: "Frequent multiplication of chromosomal region 8q24.1 associated with aggressive histologic types of breast cancers." Cancer Lett.137. 1-7 (1999)
• [Publications] Zhao,J.: "Expression of NPAT.a novel substrate of cyclin E-CDK2.promotes S-phase entry." Genes & Dev.12. 456-461 (1998)
• [Publications] Sasaki,T.: "ATM mutations in patients with ataxia telangiectasia screened by a hierarchical strategy." Hum.Mutat.12. 186-195 (1998)
• [Publications] Yamagishi,F.: "Molecular analysis of chromosome 14q32 abnormalities in esophageal and gastric carcinoma." Annats of Cancer Research and Therapy. 6. 85-88 (1998)
• [Publications] Sakashita,A.: "Amplification of the TCLI flanking region at 14q32.1 with no TCLI gene transcription in a patient with peripheral T-cell lymphoma." Leukemia. 12. 970-971 (1998)
• [Publications] Sugimoto,J.: "Isolation and mapping of a polymorphic CA repeat sequence at the human VRK1 locus." J.Hum.Genet.(in press).
• [Publications] Bando,T.: "Loss of heterozygosity of chromosome 14q32 in colorectal carcinoma." Cancer Genet.Cytogenet.(in press).
• [Publications] Sugimoto,J.: "Isolation and mapping of a putative b subunit of human ATP synthase (ATP-BL)from human leukocytes." DNA Res.(in press).
• [Publications] Yamauchi,T.: "Structural organization of the human EIK1 gene and its processed pseudogene EIK2." DNA Res.(in press).
• [Publications] Yatsuoka,T.: "Genomic analysis of the thymine-DNA glycosylase (TDG) gene on 12q22-q24.1 in human pancreatic ductal adencarcinoma ." Int.J.Pancreatol.(in press).
• [Publications] Abe,T: "Identification of three commonly deleted regions on chromosome arm 6q in human pancreatic cancer." Genes.Chrom.Cancer.(in press).
• [Publications] Furukawa,T.: "Genomic analysis of DUSP6,a dual specificity MAP kinase phosphatase,in pancreatic cancer." Cytogenet.Cell.Genet.82. 156-159 (1998)
• [Publications] Yoshinaga,K.: "The PTEN,BAX and IGFIIR genes are mutated in endometrial atypical hyperplasia." Jpn.J.Cancer Res.89. 985-990 (1998)
• [Publications] Yamakawa,H.: "Identification of a 100-kb region of common allelic loss on chromosome bands 10q25-q26 in human endometrial cancer." Genes.Chrom.Cancer.23. 74-77 (1998)
• [Publications] Kimura,M.: "Identification of two common regions of allelic loss in chromosome arm 12q in human pancreatic cancer." Cancer Res.58. 2456-2460 (1998)
• [Publications] Sasaki,N.: "Transcriptional sequencing: A method for DNA sequencing using RNA polymerase." Proc.Natl.Acad.Sci.,USA. 95. 3455-3460 (1998)
• [Publications] Izawa,M.: "Recognition sites of 3'-OH group by T7 RNA polymerase and its application to transcriptional sequencing." J.Biol.Chem.273. 14242-14246 (1998)
• [Publications] Sasaki,N.: "Identification of stable RNA hairpins causing band compression in transcriptional sequencing and their elimination by use of inosine triphosphate." Gene. 222. 17-24 (1998)
• [Publications] Yoshikawa,H.: "A novel human gene located on Xp11.2-p11.4 which escapes X-inactivation detected and isoland using a two-dimensional DNA mapping method." Genomics. 49. 237-246 (1998)
• [Publications] Abe,T.: "Infantile leukemia and soybeans-a hypothesis." Leukemia. (in press).
• [Publications] Yashige,H.: "Micronuclei and nuclear abnomalities observed in erythroblasts in myelodysplastic syndromes and in de novo acute leukemia after treatment." Acta.Haematologica. (in press).
• [Publications] Akiyama,Y.: "Anplification on double-minute chromosomes and partial-tandem duplication of the MLL gene in leukemic cells of a patient with acute myelogenous leukemia." Genes.Chrom.Cancer. 23. 267-272 (1998)
• [Publications] Kishi,T.: "Hematopoietic cytokine-dependent differentiation to eosinophils and neutrophils in a newly established acute promyelocytic leukemia cell line with t(15;17)." Experimental Hematol.26. 135-142 (1998)
• [Publications] Chikayama,S.: "Effects of daunorubicin on cell growth,cell cycle and induction of apoptosis in HL-60 cells." Hematologia. 29. 115-121 (1998)
• [Publications] Shiina,T.: "Nucleotide sequencing determination of the 1.8 Mb entire HLA class 1 region." Monduzzi Editore.Bologna, 5 (1998)
• [Publications] Shimizu,N.: "Human Genome Project: Current Status,Keio Strategy and Prospects." Springer/Verlag,Tokyo, 6 (1998)
• [Publications] Hayashi,K.: "Single strand comformation polymorphism analysis,in Mutation Detection: A Practical Approach" Oxford University Press.Oxford, 18 (1998)
• [Publications] Myers,R.M.: "Detection of Mutations,in Genome Analysis" Cold Spring Harbor Laboratory Press, 98 (1998)
• [Publications] Hosoda,F.: "A complete Notl restriction map covering the entire long arm of human chromosome 11." Genes to Cells. 2. 345-357 (1997)
• [Publications] Kitamura,E: "A 3-Mb sequence-ready contig map encompassing the multiple disease gene cluster on chromosome 11q13.1-13.3." DNA Res.4. 281-289 (1997)
• [Publications] Kitabayashi,I: "The AMLI,MTG8 leukemic fusion protein forms a complex with a novel member of the MTG8(ETO/CDR)family,MTGRI." Mol.Cell.Biol.18. 846-858 (1998)
• [Publications] Ohira,M.: "Gene identification in 1.6-Mb region of the Down syndrome region on chromosome 21." Genome Res.7. 47-58 (1997)
• [Publications] Arai,Y.: "The inv(II)(p15q22)chromosome translocation of de novo and therapy-related myeloid malignancies results in fusion of the nucleoporin gene,NUP98with the putative RNA helicase gene,DDX10." Blood. 89. 3936-3944 (1997)
• [Publications] Hattori,M.: "A novel method for making nested deletins and its application for sequencing of a 300 kb region of human APP locus." Nucleic Acids Res.25. 1802-1808 (1997)
• [Publications] Nakamura,A.: "Isolation of a novel human gene from the Down syndrome critical region of chromosome 21q22.2." J.Biochem.122. 872-877 (1997)
• [Publications] Hsgiwama,Y.: "Screening for imprinted genes by allelic messge display:identification of a paternally expressed gene Impact on Mouse chromosome 18." Proc.Natl.Acad.Sci.USA. 96. 9249-9254 (1997)
• [Publications] Tei,H.: "Circadian oscillation of a mammalian homorogue of the Drosophila period gene" Nature. 389. 512-516 (1997)
• [Publications] Mizuki,N.: "Triplet repeat polymorphism in the transmembrane region of the MICA gene:A strong association of six GCT repetitions with Behcet's disease." Proc.Natl.Acad.Sci.USA. 94. 1298-1303 (1997)
• [Publications] Mizuki,N.: "Nucleotide sequene analysis of the HLA class I region spanning the 237kb segment around the HLA-B and C gene." Genomics. 42. 55-66 (1997)
• [Publications] Kikuti,Y.: "Physical mapping 220kb centromeric of the human MHC and DNA sequencing analysis of the 43-kb segment including the RING1,HKE6,and HKE4 genes." Genomics. 42. 422-435 (1997)
• [Publications] Tanzen,T.: "Precise switching of DNA replication timing in the GC content transition area in the human MHC." Mol.Cell.Biol.17. 4043-4050 (1997)
• [Publications] Endo,T.: "Evolutionary significance of intra-genome duplications on human chromosomes." Gene. 205. 19-27 (1997)
• [Publications] Iwahara,J.: "A helix-turn-helix structure unit in human centromere protein B(CENP-B)." EMBO J.17. 827-837 (1998)
• [Publications] Oyake,M.: "Molecular cloning of murine homologue dentatorubral-pallidoluysian atrophy(DRPLA)cDNA:Strong conservation of a polymorphic CAG repeat in the murine gene." Genomics. 40. 205-207 (1997)
• [Publications] Illarioshkin,S.: "Refined genetic location of the chromosome 2p-linked progressive muscular dystrophy gene." Genomics. 42. 345-348 (1997)
• [Publications] Igarashi,S.: "Suppression of aggregate formation and apoptosis by transglutaminase inhibitors in cells expressing truncated DRPLA protein with an expanded polyglutamine stretch." Nature Genet.18. 111-117 (1998)
• [Publications] Kawasaki,K: "One megabase sequence analysis of the human immunoglobulin γ gene locus." Genome Res.7. 250-261 (1997)
• [Publications] Imamura,Y.: "Human retina-specific amine oxidase(RAO):cDNA cloning,tissue expression and chromosomal mapping." Genomics. 40. 277-283 (1997)
• [Publications] Chrast,R.: "Cloning of two human homologs of the Drosophila single-minded gene SIM1 on chromosome 6q and SIM2 on 21q within the Down syndrome chromosomal region." Genome Res.7. 615-624 (1997)
• [Publications] Nagamine,K.: "Positional cloning of the APECED gene." Nature Genet.17. 393-398 (1997)
• [Publications] Miyake,M.: "YAC and cosmid contigs encompassing the Fukuyama-type congenital muscular dystrophy(FCMD)candidate region on 9q31." Genomics. 40. 284-293 (1997)
• [Publications] Kondo-Iida,E.: "Molecular genetic evidence of clinical heterogeneity in Fukuyama type congenital muscular dystrophy." Hum.Genet.99. 427-432 (1997)
• [Publications] Saito,K.: "Prental diagnosis in eight Fukuyama type congenital muscular dystrophy families by haplotype analysis using the new markers closest to the gene." Am.J.Med.Genet.(in press).
• [Publications] Matsumoto,N.: "A 1.2-megabase BAC/PAC contig spanning the 14q13 breakpoint of t(2;14)in a minor-image polydactyly patient." Genomics. 45. 11-16 (1997)
• [Publications] Fujimoto,M.: "The gene for mesomelic dysplasia Kantaputra type is mapped to 2q24-q32." J.Hum.Genet.(in press).
• [Publications] Matsumoto,S.: "Detailed deletion mapping of chromosome arm 3p in breast cancrers:A 2-cM region on 3p14.3-21.1 and a 5-cM region on 3p24.3-25.1 commonly deleted in tumors." Genes,Chrom.Cancer. 20. 268-274 (1997)
• [Publications] Iida,A.: "Mapping of a new target region of allelicloss to a 2-cM interval at 22q13.1 in primary breast cancer." Genes,Chrom.Cancer. 21. 108-112 (1998)
• [Publications] Imai,T.: "The structure and organization of the human NPAT gene." Genomics. 42. 388-392 (1997)
• [Publications] Hori,T.: "A distamycin A-inducible fragile sote,FRA8E,is located in the hereditary multiple exostoses gene and not involved in HPV16 DNA integration and amplification." Cancer Genet.Cytogenet.99. 1-11 (1997)
• [Publications] Yamagishi,F.: "Molecular analysis of chromosome 14q32 abnormalities in esophageal and gastric carcinoma." Annals of Cancer Res. and Therapy. (in press).
• [Publications] Sakashita,A.: "Amplificatin of the TCL1 franking region af 14q32.1 with no TCL1 gene transcription in a patient with peripheral T-cell lymphoma." Leukemia. (in press).
• [Publications] Kimura,M.: "Identification of two common regions of allelic loss in chromosome arm 12q in human pancreatic cancer." Cancer Res.(in press).
• [Publications] Kong,D.: "PTENI is frequently mutated in primary endometrial carcinomas." Nature Genet.17. 143-144 (1997)
• [Publications] Yamane,Y.: "Allelic frequencies of twelve dinucleotide repeat marker loci o n chromosome 13 in the normal Japanese population." Jpn.J.Hum.Genet.42. 533-537 (1997)
• [Publications] Chinene,K.: "Isolation of 45 ex on-like fragments from 8p21.3-p22,a region that is commonly deleted in hepatocellular,colorectal,and non-small cell lung carcinomas." Cytogenet.and Cell Genet.190-196 (1997)
• [Publications] Ito,M.: "Simple and rapid preparation of plasmid template by a filtration method using microtiterfilter plates." Nucleic Acids.Res.25. 1315-1316 (1997)
• [Publications] Yoshkawa,H.: "Chromosome assignment of abrrant Natl restriction DNA fragments in primary hepatocellular carcinoma." Gene. 197. 129-135 (1997)
• [Publications] Iha,H.: "Identification and characterization of a novel trans-membrane protein gene,pdh1,from Schizosaccharomyces pombe." DNA Res.4. 393-396 (1997)
• [Publications] Kimura,T.: "The brain finger protein gene(znf179),a memter of the RING finger family,maps within the Smith-Magenis syndrome region at 17q1.2." Am.J.Med.Genet.69. 320-324 (1997)
• [Publications] Hiroike,S.: "Tandem duplications of the FLT3 Receptor gene are closely associated with leukemic transformation of myelodysplasia." Leukemia. 11. 1442-1446 (1997)
• [Publications] Ueda,Y.: "Interphase detection of BCL6/IgH fusion gene in non-hodgkin lymphoma by fluoredsence in situ hybridization." Cancer Genet.Cytogenet.99. 102-107 (1997)
• [Publications] Kukita,Y.: "SSCP analysis of long DNA fragments in low PH gel." Hum an Mutation. 10. 400-407 (1997)
• [Publications] Inazuka,M.: "A stream-lined mutation detection system:Multi-color post-PCR fluorescence-labeling and SSCP analysis by calillary electrophoresis." Genome Res.7. 1094-1103 (1997)
• [Publications] Ohira.M.: "Identification of a novel gene containing the tetratricopeptide repeat domain from the Down syndrome region of chromosome 21." DNA Res.3. 65-74 (1996)
• [Publications] Arai.Y.: "A Yeast artificial chromosome contig and Not I restriction map that spans the tumor suppressor gene (s) locus.11q22.2・23.3." Genomics. 35. 196-206 (1996)
• [Publications] Morohoshi.F.: "Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS protein." Genomics. 38. 51-57 (1996)
• [Publications] Naruse.K.: "A YAC contig of the human CC chemokine gene clusterd on chromosome 17q11.2." Genomics. 34. 236-240 (1996)
• [Publications] Tsukahara.F.: "Identification and cloning of a novel cDNA belonging to tetratricopeptide repeat gene family from Down syndrome-critical region 22q22.2." J.Biochem.120. 820-827 (1996)
• [Publications] Shibata.A.: "Identification of cis-acting elements involved in an alternative splicing of the amyloid precursor protein (APP) gene." Gene. 175. 203-208 (1996)
• [Publications] Hattori,M.: "Novel Method for making nested deletions and its application for sequencing of a 300kb APP gene locus." Nucleic Acid Res.in press.
• [Publications] Kuro,J.: "Localization of 16 exons to a 450-kb region involved in the autoimmune polyglandular disease type I (Apeced) on human chromosome 21q22.3." DNA Res.in press.
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• [Publications] Ueda,Y.: "Tumor-specific rearrangements of the immunoglobulin heavy-chain gene in B-cell Non-Hodgkin's lymphoma detected by in situ hybridization." Blood. 87. 292-298 (1996)
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• [Publications] Ishihara,M.: "Genetic polymorphisms of the MHC-encoded antigen processing gene (TAP,LMP) in sarcoidosis." Hum.lmmunl.45. 105-110 (1996)
• [Publications] Kasahara,M.: "Chromosomal localization of the proteosome Z subunit gene reveals an ancient chromosomal duplication involving the major histocompatibility complex." Proc.Natl.Acad. sci,.USA. 93. 9096-9101 (1996)
• [Publications] Mizuki,N.: "Triplet repeat polymorphism in the transmembrane region of the MICA gene : A strong association of six GCT repetitions with Behcet disease." Prec.Natl.Acad.Sci,.USA. 94. 1298-1303 (1997)
• [Publications] Takano,H.: "Somatic mosaicism of expanded CAG repeats in brains of patients with dentatorubral-pallidoluysianatrophy : Cellular population-dependent dynamics of mitotic instability." Am.J.Hum.Genet.58. 1212-1222 (1996)
• [Publications] Igarashi,S.: "Intergenerational instability of the CAG repeat of the gene for Machado-Joseph disease (MIDI) is affected by the genotype of the normal chromosome : implications for the molecular mechanisms of the instability of the CAG repeat." Hum.Mol.Genet.5. 923-932 (1996)
• [Publications] Sanpei,K.: "Identification of the spinocerebellar ataxia type 2 gene using a derect identification of repeat expansion and cloning technique,DIRECT." Nature Genet.14. 277-284 (1996)
• [Publications] Toda,T.: "Linkage-disequilibrium mapping narrows the Fukuyama-type congenital muscular dystrophy (FCMD) candidate regin to<100kb." Am.J.Hum.Genet.59. 1313-1320 (1996)
• [Publications] Miyaka,M.: "YAC and cosmid contigs encompassing the Fukuyama-type congenital muscular dystrophy (FCMD) candidate region on 9q31." Genomics. in press.
• [Publications] Ohta,T.: "Isolation of a cosmid clone corresponding to a region of the inv (21) breakpoint in a patient with transient abormal myelopoiesis." Am.J.Hum.Genet.58. 544-550 (1996)
• [Publications] Iida,A.: "Localization of a breast cancer tumor-suppressor gene to a 3-cM interval within chromosomal region 16q22." Brit.J.Cancer. 75. 264-267 (1997)
• [Publications] Suzuki,H.: "Three distinct commonly deleted regions of chromosome arm 16q in human primary and metastatic prostate cancers." Genes,Chrom.Cancer. 17. 225-233 (1996)
• [Publications] Imai,T.: "Identification and characterization of a new gene physically linked to ATM gene." Genome Res.6. 439-447 (1996)
• [Publications] Caminci,P.: "High-efficiency full-length cDNA cloning by biotinylated CAP trapper." Genomics. 37. 327-336 (1996)
• [Publications] Yoshikawa,H.: "Chromosome assignment of human genomic Not I restriction fragments in a two-dimensional electrophoresis profile." Genomics. 31. 28-35 (1996)
• [Publications] Ichikawa,D.: "Analysis of numerical aberrations in specific chromosomes by fluorescence in situ hybridization (FISH) as a diagnostic tool in breast cancer." Cancer. 77. 2064-2069 (1996)
• [Publications] Kumura,T.: "The brain finger protein gene (ZNF197),a mumber of the RING finger family,maps within the Smith-Magenis syndrome region in 17p11.2." Am.J.Med.Genet.in press.
• [Publications] Inazuka,M.: "One-tube post-PCR fluorescent labeling of DNA fragments." Genome Res.6. 551-557 (1996)
• [Publications] Kanbara,H.: "Fragment walking for long DNA sequencing by using a library as small as 16 primers." Gene. 176. 231-235 (1996)
• [Publications] Matsuda.F.: "Organization of the Human lmmunoglobulin Heavy-Chain Locus." Academic Press, 1-29 (1996)
• [Publications] Hayashi.K.: ""PCR・SSCP".in "Laboratory Protocol for Mutation Detection"" Oxford University Press, 14-22 (1996)