| Project/Area Number |
08300012
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Institution | Osaka University |
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Principal Investigator |
HONDA Takeshi Biken.Osaka Univ.Prof., 微生物病研究所, 教授 (60029808)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Shinji Int.Med.Center Chief Div., 室長 (70221653)
TAKEDA Tae Nat.Child.Med.Res.Center Dir., 部長
YOSHIDA Shin-ichi Occup.Environ.Health Univ.Prof., 教授 (60128113)
IGARASHI Takashi Tokyo Univ.Med.Hospital Assis.Prof., 医学部・付属病院, 講師 (70151256)
OGUMA Keiji Okayama Univ.Med.Prof., 医学部, 教授 (00002262)
竹田 多恵 国立小児病院医療研究センター, 部長
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| Project Period (FY) |
1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Keywords | Escherichia coli / EHEC O157 / Epidemiology / Pathogenesis / Diagnosis / Treatment |
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| Research Abstract |
1.Epidemiology : Survey for EHEC in cattle, especially calf, was conducted in Okayama and Sizuoka prefectures and we found that about 0.1-1.0% of calf tested were positive for EHEC O157. The isolates were tested for biochemical character, antimicrobial sensitivity test, and molecular analyzes including PFGE.A questionnaire survey on EHEC infection was also conducted for clinicians around Osaka prefecture.
2.Pathogenesis : Several animal models such as mouse, rat and rabbit were established. Those experiments suggested that VT showed direct toxicity to brain as well as renal tubular cells.
3.Diagnosis : Molecular genetical techniques such as DNA-probe and PCR methods were developed for EHEC detection. Latex-oriented lactofferin (an indicator for neutrophil) assay using diarrheal stool was applied for quick suspicion of EHEC infection. Some detection kits for VT and EHEC O157 were also evaluated. Thrombomodulin in sera was revealed to be an indicator for early recongnition of HUS.
4.Treatment : It was shown that anti-VT antiserum prevented mouse from lethality due to VT.Effect of anti-microbial agents on VT-production by EHEC was examined.
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