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Molecular analyzes of the mechanism of plant virus pathogenicity

Research Project

Project/Area Number 08306003
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

FURUSAWA Iwao  Kyoto University, Guraduate School of Agriculuture, Professor, 農学研究科, 教授 (10026594)

Co-Investigator(Kenkyū-buntansha) WATANABE Yuichirou  Department of Life Science, Graduate School of Arts and Sciences, University of, 総合文化研究科生命環境科学系, 助教授 (60183125)
ISHIKAWA Masayuki  Hokkaido University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70192482)
HOSOKAWA Daijirou  Tokyo University of Agriculuture and Technology, Professor, 農学部, 教授 (50014957)
UEDA Ichiro  Hokkaido University, Faculty of Agriculture, Professor, 農学部, 教授 (10113523)
HIBI Tadaaki  University of Tokyo, Faculty of Agriculture, Professor, 農学部, 教授 (50261954)
本吉 総男  岡山大学, 資源生物学研究所, 教授 (90230052)
吉川 信幸  岩手大学, 農学部, 助教授 (40191556)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥24,800,000 (Direct Cost: ¥24,800,000)
Fiscal Year 1997: ¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 1996: ¥14,400,000 (Direct Cost: ¥14,400,000)
KeywordsCaMV / TMV / CMV / PVX / ToMV / RNA polymerase / host factor / host specificity / RDV / RNA複製酵素 / 植物ウイルス / ゲノム解析 / 病原性 / 遺伝子 / ポリメラーゼ / 全身感染
Research Abstract

We have comprehensively studied the mechanism of genome replication of various plant viruses. Chronological analyzes of the accumulation of viral gene products in cauliflower mosaic virus (CaMV)-infected turnip protoplasts showed that the expression of each gene is differentially regulated. It has also been suggested that subgenomic RNAs as well as the polycistronic 35S RNA are involved in the expression of CaMV genes, indicating that CaMV uses a unique strategy for the gene expression during virus multiplication. Two domains of the 180K protein gene of tobacco mosaic virus (TMV)-OM,each containing the sequence motifs of methyltransferase or RNA polymerase, have shown to exhibit the binding activity with TMV-RNA.By immunoaffinity chromatography using antibodies specfic to the domains, heterodimers consisting of 130K and 180K viral proteins, which exhibited TMV-RNA dependent RNA synthesis in vitro, were isolated from TMV-infected tobacco leaves. We have analyzed Arabidopsis thaliana mutants designated cum1 and cum2, in which the multiplication of cucumber mosaic virsu (CMV) is delayd. Furthermore, we have been trying to clone the CUM1 gene by the positional cloning procedure. We have isolated solubilized RNA polymerase synthesizing subgenomic RNA from tobacco protoplasts inoculated with potato virus V (PVX). Amino acid 979 of the 130-and 180-kDa proteins of tomato mosaic tobamovirus (ToMV) plays an important role in overcoming the tomato Tm-1 resistance gene. Introduction of a single amino acid substitution (Gln979 to Ile) into the ToMV proteins rendered the mutant virtually unable to replicate in tomato cells while it replicated perfectly in tobacco cells, indicating the importance of the region surrounding the amino acid 979 in terms of host specificity determinants.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Kappei Kobayashi: "Accumuiation Kinetics of Viral Gene Products in Cauliflower Mosaic Virus-Infected Turnip Protoplasts" Microbiol.Immunol.42(1). 65-69 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 海道 正典: "Auto-cleavable Ribozyme Sequence Attached to Brome Mosaic Virus cDNA As Enhances Accumulation of Viral RNAs Transcribed In Vivo from the cDNAs" 日本植物病理学会報. 63(2). 95-98 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hideaki Nagano: "Deletion of the C-Terminal 33 Amino Acid of Cucumber Mosaic Virus Movement Protein Enables a Chimeric Brome Mosaic Virus To Move from Cell to Cell" Journal of Virology. 71(3). 2270-2276 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kappei Kobayashi: "Accumuiation on Kinetics of Viral Gene Products in Cauliflower Mosaic Virus-Infected Turnip Protoplasts" Microbiol.Immunol.42(1). 65-69 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masanori Kaido: "Auto-cleavable Ribozyme Sequence Attached to Brome Mosaic Virus cDNA As Enhances Accumulation of Viral RNAs Transcribed In Vivo from the cDNAs" Ann.Phytopathol.Soc.Jpn.63(2). 95-98 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hideaki Nagano: "Deletion of the C-Terminal 33 Amino Acids of Cucumber Mosaic Virus Movement Protein Enables a Chimeric Brome Mosaic Virus To Move from Cell to Cell" Journal of Virology. 71(3). 2270-2276 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kappei Kobayashi: "Accumuiation Kinetics of Viral Gene Products in Cauliflower Mosaic Virus-Infected Turnip Protoplasts" Microbiol.Immunol.42(1). 65-69 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 海道正典: "Auto-cleavable Ribozyme Sequence Attached to Brome Mosaic Virus cDNA As Enhances Accumulation of Viral RNAs Transcribed In Vivo from the cDNAs" 日本植物病理学会報. 63(2). 95-98 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yukio Shirako: "Requirement for an Aromatic Amino Acid or Hisidine at the N Terminus of Sindbis Virus RNA Polymerase" Journal of Virology. 72(3). 2310-2315 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroshi Hamamoto: "Amino acid changes in the putative replicase of tomato mozaic tobamovirus that overvome resistance in Tm-1 tomato" Journal of General Virology. 78. 461-464 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroshi Hamamoto: "A Single Amino Acid Substitution in the Virus-Encoded Replicase of Tomato Mosaic Tobamovirus Alters Host Specificity" Molecular Plant-Microbe Interactions. 10(8). 1015-1018 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masayuki Ishikawa: "In VIvo DNA Expression of Functional Brome Mosaic Virus RNA Replicons in Saccharomyces cerevisiae" Journal of Virology. 71(10). 7781-7790 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masayuki Ishikawa: "Yeast mutation ins multiple complementation groups inhibit brome mosaic virus RNA replication and transcription and perturb regulated expression of the viral polymerase-like gene" Proc.Natl.Acad.Sci.USA. 94. 13810-13815 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shigenori Ueda: "Hypothesis on particle structure and assembly of rice dwarf phytoreovirus : interactions among multiple structural proteins" Journal of General Virology. 78. 3135-3140 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoko Takahashi: "A cDNA Clone to Clover Yellow Vein Potyvirus Genome is Highly Infectious" Virus Genes. 14(3). 235-243 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] B.C.Lee: "Phylogenetic relationships between rice dwarf phytoreovirus isolates from five countries" European Journal of Plant Pathology. 103. 493-499 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] I.Uyeda: "The rice dwarf phytoreovirus structural protein P7 possesses non-specific nucleic acids binding activity in vitro" Molecular Plant Pathology. 1997. (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Motoyashi Yoshii: "Isolation of an Arabidopsis thaliana mutant in Which accumulation of cucumber mosaic virus coat protein is delayed" The Plant Journal. 13(2). 211-219 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.K.Kundu: "Nucleotide sequences of the coat protein genes of two Japanese Zucchini yellow mosaic virus isolates" Acta virologica. 41. 297-301 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yukio Shirako: "Non-AUG Translation Initiation in Plant RNA Virus : a Forty-Amino-Acid Extension Is Added to the N Terminus of the Soil-Borne Wheat Mosaic Virus Capsid Protein" Journal of Virology. 72(2). 1677-1682 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomohisa Kuroda: "Formation of multimer of cucumber mozaic virus satelite RNA" Journal of General Virology. 78. 941-946 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Talahide Sasaya: "Characterization and Nucleotide Sequence of the 3' Terminal Region of Clover Yellow Vein Isolated from Gentian (Gentiana spp.)" 日本植物病理学会報. 63(4). 328-333 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Akira Karasawa: "A Possible Role of RNA 2 of Cucumber Mosaic Cucumovirus as a Determinant of Infection Phenotype on Cowpea" 日本植物病理学会報. 63(4). 289-297 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Magome: "Molecular Variability of the Genomes of Capilloviruses from Apple,Japanene Pear,European Pear,and Citrus Trees" Molecular Plant Pathology. 87(4). 389-396 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] N.Yoshikawa: "Grapevine berry inner necrosis,a new trichovirus : comparative studies with several known trichoviruses" Archives of Virology. 142. 1351-1363 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hidetaka Terauchi: "Construction of an Infectios cDNA Clone of the Apple Stem Grooving Capillovirus (Isolate Li-23) Genome Containing a Cauliflower Mosaic Virus 35S RNA Promoter" 日本植物病理学会報. 63(6). 432-436 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroshi Magome: "Analysis of Double-stranded RNA in Tissues Infected with Apple Stem Grooving Capillovirus" 日本植物病理学会報. 63(6). 450-454 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 佐藤正幸: "キュウリモザイクウイルス3分離株のダイコン品種における感染と増殖" 九州病害虫研究会報. 43. 39-41 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Minoru Takeshita: "Complete Nucleotide Sequences of RNA3s of Cucumber Mosaic Virus KM and D8 Strains" J.Fag.Agr..,Kyusyu Univ.42(1・2). 27-32 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kiyoshi Ohshima: "Isolation of mutant of Arabidopsis thaliana carrying two simulataneous mutations affecting tobacco mosaic virus multiplication within a single cell" Virology. accepted

    • Related Report
      1997 Annual Research Report
  • [Publications] Takuya Yamanaka: "Complete nucleotide sequence of the genomic RNA of tobacco mosaic virus strain Cg" Virus Genes. in press

    • Related Report
      1997 Annual Research Report
  • [Publications] Shiro Kuninaga: "Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani" Curr Genet. 32. 237-243 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Ohmori: "Characterzation of disease resistance gene-like sequences in near isogenic lines of tomato" Theor Apple Genet. 96. 000-000 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 古澤 巌: "「ウイルス学」(畑中正一編集)植物ラブドウイルスpp.468-470" 朝倉書店、東京, 646 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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