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Screening, structure and usefulness of marine bioactive proteins

Research Project

Project/Area Number 08306011
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTokyo University of Fisheries

Principal Investigator

SHIOMI Kazuo  Tokyo University of Fisheries, Faculty of Fisheries., Professor, 水産学部, 教授 (90111690)

Co-Investigator(Kenkyū-buntansha) SUZUKI Yuzuru  The University of Tokyo, Graduate School, Associate Professor, 大学院・農学生命科学研究科, 助教授 (40107412)
TAKAMATSU Nobuhiko  Kitasato University, Faculty of Science, Associate Professor, 理学部, 助教授 (40206876)
HORI Kanji  Hiroshima University, Faculty of Applied Biological Science, Associate Professor, 生物生産学部, 助教授 (50116662)
MURAMOTO Koji  Tohoku University, Faculty of Agriculture, Professor, 農学部, 教授 (90157800)
KAMIYA Hisao  Kitasato University, Faculty of Fisheries, Professor, 水産学部, 教授 (80011964)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 1997: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1996: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsMarine bioactive proteins / Peptide toxins / Protease inhibitors / Lectins / Mitogens / Antibacterial and antitumor proteins / Amino acid sequence / Gene
Research Abstract

1. Two Na^+ channel toxins, one K^+ channel toxin, four novel peptide toxins and eight Kunitz-type protease inhibitors were isolated from bodies or acrorhagi of several species of sea anemones and completely sequenced.
2. Acorn barnacle Megabalanus rosa lectins, BRA2 and BRA3, were successfully expressed in Escherichia coli. The sugara-binding specificity of recombinant BRA3 was the same as that of native BRA3, while recombinant BRA2 showed different sugar-binding specificity from native BRA2 probably due to the absence of carbohydrate chain in the former.
3. Two rhamnose-binding lectins (STLs) were isolated from steelhead trout Oncorhynchus mykiss eggs. Sequence determinations revealed that STLs belong to a new family of animal lectins.
4. A mitogenic lectin (ESA-2) recognizing specifically high mannnose type N-glycans was isolated from the red alga Euchemuaserra. It contained several repeated sequences in the molecule, showing high sequence similarity with some bacterial hemagglutinins. Furthermore, it inhibited the growth of cultured human tumor cells.
5. An aplysianin-P (antibacterial glycoprotein from the purple fluid of sea hare Aplysia kurodai) cDNA clone was not obtained by either RT-PCR method or screening of cDNA libraries from purple gland, albumen gland and midgut. This may suggest that the gene of aplysianin-P is not expressed in the tissues examined or that the previouly determined amino acid sequence of aplysianin-P is incorrect.
6. Hemolytic and lectin activities of the eel skin mucus decreased with the rise of antibody activity in the blood plasma. Purified skin mucus lectin was found to have both opsonic and chemotactic activities against own neutrophiles. These results mean the cooperative action of surface and inner defense mechanisms. High disease susceptibility of larvae was seen in abalone born from a mother of low lectin activity, suggesting that the lectin activity is one of the characters of disease resistance in abalone.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] M.Ishida: "Halcurin,a polypeptide toxin in the sea anemone Halcurias sp.,with a structural resemblance to type 1 and 2 toxins" Toxicon. 35. 537-544 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiomi: "Novel polypeptide toxins with crab toxicity from the sea anemone Anemonia erythraea" Biochim.Biophys.Acta. 1335. 191-198 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Ishida: "Amino acie sequences of Kunitz-type protease inhibitors from the sea anemone Actinia equina" Fisheries Sci.63. 794-798 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Minagawa: "Isolation and amino acid sequences of two Kunitz-type protease inhibitors from the sea anemone Anthopleura aff.xanthogrammica" Comp.Biochem.Physiol.118B. 381-386 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Minagawa: "Amino acid sequence and biological activities of another Kunitz-type protease inhibitor from the sea anemone Anthopleura aff.xanthogrammica" Fisheries Sci.64. 155-159 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiomi: "Screening of toxins and protease inhibitors in sea anemones" Fisheries Sci.64. 170-171 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiomi: "Purification and properties of phospholipase A2 from the crown-of-thorns starfish(Acanthaster planci)venom" Toxicon. 36(印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Goto-Nance: "Purification and characterization of the lectins of soft coral Lobophytum variatum" Fisheries Sci.62. 297-301 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Muramoto: "Analysis of the hemolymph proteins from the acorn barnacle Megabalanus rosa by two-dimensional gel electrophoresis" Fisheries Sci.62. 435-438 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Toda: "Characterization of the lectin from the skin mucus of the kingklip Genypterus capensis" Fisheries Sci.62. 138-141 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Wako: "Angiotensin I-converting enzyme inhibitors in autolysate of squid liver and mantle muscle" Biosci.Biotech.Biochem. 60. 1353-1355 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kwakubo: "The marine red alga Eucheumaserra J.Agardh,a high yielding source of two isolectins" J.Appl.Phycol.9. 331-338 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Ishida, Halcurin: "a polypeptide toxin in the sea anemone Halcurias sp., with a structural resemblance to type 1 and 2 toxins" Toxicon. 35. 537-544 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiomi: "Novel polypeptide toxins with crab toxicity from the sea anemone Anemonia erythraea" Biochim.Biophys.Acta.1335. 191-198 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Ishida: "Amino acie sequences of Kunitz-type protease inhibitors from the sea anemone Actinia equina" Fisheries Sci.63. 794-798 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Minagawa: "Isolation and amino acid sequences of two Kunitz-type protease inhibitors from the sea anemone Anthopleura aff.xanthogrammica" Comp.Biochem.Physiol.118B. 381-386 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Minagawa: "Amino acid sequence and biological activites of another Kunitz-type protease inhibitor from the sea anemone Anthopleura aff.xanthogrammica" Fisheries Sci.64. 155-159 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiomi: "Screening of toxins and protease inhibitors in sea anemones" Fisheries Sci.64. 170-171 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Shiomi: "Purification and properties of phospholipase A_2 from the crown-of-thorns starfish (Acanthaster planci) venom" Toxicon. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Goto-Nance: "Purification and characterization of the lectins of the soft coral Lobophytum variatum" Fisheries Sci.62. 297-301 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Muramoto: "Analysis of the hemolymph proteins from the acorn barnacle Megabalanus rosa by two-dimensional gel electrophoresis" Fisheries Sci.62. 435-438 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Toda: "Characterization of the lectin from the skin mucus of the kingklip Genypterus capensis" Fisheries Sci.62. 138-141 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Wako: "Angiotensin I-converting enzyme inhibitors in autolysate of squid liver and mantle muscle" Biosci.Biotech.Biochem.60. 1353-1355 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kawakubo: "The marine red alga Eucheuma serra J.Agardh, a high yielding source of two isolectins" J.Appl.Phycol.9. 331-338 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Ishida: "Halcurin, a polypeptide toxin in the sea anemone Halcurias sp., with a structural resemblance to type 1 and 2 toxins" Toxicon. 35(4). 537-544 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Shiomi: "Novel polypeptide toxins with crab toxicity from the sea anemone Anemonia erythraea" Biochim.Biophys.Acta. 1335. 191-198 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Ishida: "Amino acie sequences of Kunitz-type protease inhibitors from the sea anemone Actinia equina" Fisheries Sci.63(5). 794-798 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Minagawa: "Isolation and amino acid sequences of two Kunitz-type protease inhibitors from the sea anemone Anthopleuraaff. xanthogrammica" Comp.Biochem.Physiol.118B(2). 381-386 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Minagawa: "Amino acid sequence and biological activities of another Kunitz-type protease inhibitor from the sea anemone Anthopleuraaff.xanthogrammica" Fisheries Sci.64(1). 155-159 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Shiomi: "Screening of toxins and protease inhibitors in sea anemones" Fisheries Sci.64(1). 170-171 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Shiomi: "Purification and properties of phospholipase A_2 from the crown-of-thorns starfish(Acanthaster planci) venom" Toxicon. 36(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Kawakubo: "The marine red alga Eucheuma serra J.Agardh, a high yielding source of two isolectins" J.Appl.Phycol.9. 331-338 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kazuo Shiomi: "Halcurin,a polypeptide toxin in the sea anemone Halcurias sp.,with a structural resemblance to both types 1 and 2 toxins" Toxicon. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazuo Shiomi: "Novel polypeptide toxins with crab toxicity from the sea anemone Anemonia erythraea" Biochim.Biophys.Acta. (印刷中).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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