| Project/Area Number |
08307012
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NODA Masaki Tokyo Medical and Dental University, Medical Research Institute, Professor, 難治疾患研究所, 教授 (50231725)
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| Co-Investigator(Kenkyū-buntansha) |
NOSE Kiyoshi Showa University, School of Pharmacy, Professor, 薬学部, 教授 (70012747)
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| Project Period (FY) |
1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1996: ¥7,200,000 (Direct Cost: ¥7,200,000)
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| Keywords | Bone Formation / Bone Resorption / Osteoblast / Chondrocyte / Mechanical Stress |
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| Research Abstract |
Bone and cartilage are supporting organs of the body while they also respond to physical stimuli to maintain the skeletal tissues. This research aimed to classify the roles of mechanical stress in the regulation of skeletal cell function and, therefore, generated a clonel cell line derived from such weight bearing tissue, articular cartilage, from transgenic mice harboring SV-40 large-T antigen gene. This large T-antigen is active at 33ﾟ and inactive at 37ﾟ. To establish chondrocyte cell lines, we cultured the cells at 33ﾟ at a low FBS concentration (0.5%). The established TC-6 cell aggregates were stained positive for alcian blue and toluidine blue, indicating production of cartilage matrix. TC-6 cells also express type II collagen, aggrecan and link protein genes. Extermal stress affects cells via cell attachment machinery. TC-6 cells attachment to bacteriological dish was examined and we found that TC-6 cells can survive in these dish. Therefore, these cells are highly able to establish attachment efficiently even in non-attaching environment and are useful to study mechanical stress and signal transduction via attachment machinery as well as growth factor effects such as those of BMP.
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