| Project/Area Number |
08307021
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 総合 |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Tokyo |
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Principal Investigator |
KUBOTA Shunichiro Department of Physiological Chemistry and Metaboliam, Graduate School of Medicine, The University of Tokyo, Associate Professor, 大学院・医学系研究科, 助教授 (00260480)
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| Co-Investigator(Kenkyū-buntansha) |
OBARA Masanobu Department of Biology, Faculty of Science, Hiroshima University, Associate Profe, 理学部, 助教授 (60132479)
HORI Hisae Institute for Diseases, Tokyo Medical and Dental University, Assistant Professor, 難治疾患研究所, 助手 (80014190)
ICHINOSE Masao First Department of Internal Medicine, Faculty of Medicine, The University of To, 医学部・付属病院・内科, 助手 (50143425)
YAMAMOTO Kazuhiko Department of Internal Medicine, The University of Tokyo, Professor, 医学部・付属病院・内科, 教授 (80191394)
SEYAMA Yousuke Department of Physiological Chemistry and Metabolism, Graduate School of Medicin, 大学院・医学系研究科, 教授 (90010082)
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| Project Period (FY) |
1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1996: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | extracellular matrix / laminin / TIMP / tumor marker / hepatocellular carcinoma / gastric cancer / prognosis / MMP |
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| Research Abstract |
We have investigated whether the expression of matrix metalloproteases (MMPs) and tissue inhibitor of metalloprotease (TIMP), and of extracellular matrix components such as laminin, and merosin are associated with status of disease such as cancer and inflammatory diseases. We have found that serum MMP-9 level was associated with advanced disease status in gastrointestinal cancers (colon and pancreas cancer), and with prognosis of gastric cancer patients. We also found that CSF MMP-2 and MMP-9 levels were associated with malignant phenotype of brain tumor such as glioblastoma. We established RT-PCR method for measurements of laminin, MMP-2, MMP-9, and TIMP mRNA expression. We also established in situ hybridization method for detection of laminin subunit expression, and applied this method for the detection of laminin subunit in human hepatocellular carcinoma. Differentiation of hepatocellular carcinoma was intimately associated with expression of laminin subunits.
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