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Molecular biology of the target signal for melanogenesis associated genes and vitiligo pathogenesis.

Research Project

Project/Area Number 08407022
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionSapporo Medical University

Principal Investigator

JIMBOW Kowichi  Sapporo Medical University, Professor, 医学部, 教授 (30094238)

Co-Investigator(Kenkyū-buntansha) ONODERA Hideo  Sapporo Medical University, Lecturer, 医学部, 講師 (10194614)
KISHI Hiroyuki  Sapporo Medical University, Assistant, 医学部, 助手 (00295354)
KATO Fumihiko  Sapporo Medical University, Assistant, 医学部, 助手 (10295355)
KONDO Seiji  Sapporo Medical University, Assistant Professor, 医学部, 助教授 (30170426)
SAGA Kenji  Sapporo Medical University, Assistant Professor, 医学部, 助教授 (10153925)
三浦 俊祐  札幌医科大学, 医学部, 講師 (50244364)
杉山 貞夫  札幌医科大学, 医学部, 助教授 (60045490)
斉藤 和哉  札幌医科大学, 医学部, 助手 (60274932)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥33,400,000 (Direct Cost: ¥33,400,000)
Fiscal Year 1998: ¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1997: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1996: ¥21,000,000 (Direct Cost: ¥21,000,000)
Keywordsmelanin / melanocyte / vitiligo / apoptosis / lysosome / 尋常性白班 / 遺伝子導入
Research Abstract

This project has studied the biogenesis of melanosomes and function of melanin pigmentation in man for better understanding of the mechanism of leukoderma (e.g., vitiligo). Specifically we have utilized various molecular biology techniques including cDNA transfection of human tyrosinase and tyrosinase-related protein (TRP-1). We have characterized (1) correct assembly and folding of tyrosinase-gene family proteins with the help of calnexin, (2) target signals of tyrosinase-gene family proteins from TGN to melanosomes, (3) functional interactionbetween tyrosinase and lysosome-associated membrane protein in melanosome biogenesis, (4) mechanism of abnormal melanin synthesis in vitiligo melanocytes and (5) chemical modification of melanin precursor and development of depigmentation. The obtained results will be useful not only for better understanding of vitiligo pathogenesis but also for development of new selective melanocytotoxic agents

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Ota A et al: "Functional regulation of tyrosinase and LAMP gene family of melano-genesis and cell death in immortal murine melanocytes after repeated exposure to UV-B" Br.J.Dermatol.139. 207-215 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Minamitsuji Y et al.: "N-propionyl-4-S-cysteaminylphenol exerts selective melanocytotoxicity through apoptotic process." Sapporo Med J.67. 23-37 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tandon M et al: "Synthesis of the melanogenesis-based antimelanoma agent, N-propionyl-4-S-cysteaminylphenol, and screening of depigmenting and anti-tumor effects" Bio Pharmacol. 55. 2023-2029 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Singh MV et al: "Expression of human tyrosinase in rat glioma cells for brain tumor therapy." Melanoma Res. (印刷中)

    • Related Report
      1998 Annual Research Report
  • [Publications] Toyofuku K et al.: "Involvement of calnexin in maturation of tyrosinase as a molecular chaperone" J.Biochem. (印刷中)

    • Related Report
      1998 Annual Research Report
  • [Publications] Minamitsuji Y et al: "Sulphur containing tyrosine analogs can cause selective melano cytotoxicity involving tyrosinase-mediated appoptosis." J Invest Dermatol. (印刷中)

    • Related Report
      1998 Annual Research Report
  • [Publications] Jimbow K: "Dermatologic Clinics" WB.Saunders Company, 8 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Jimbow K et al: "The Pigmentary System and Its Disorders." Oxford University Press, 7 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] J0116imbow K et al: "Biological role of tyrosinase related protein and its biosynthesis and transport from TGN to stage I melanosome late endosome,through gene transfection study." Pig.Cell Res.10. 206-213 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 神保孝一: "21世紀に向けてのメラニン生物学-基礎と臨床との接点、対応-" 日皮会誌. 107. 1559-1566 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Jimbow K: "Tuberous sclerosis and guttate leukodermas." Semi Cutan Med. 16. 30-35 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kondo S: "Basement membrane is not a barrier for cytokine permeability" J.Cell Physiol. 171. 190-195 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kondo S: "Modulation of TGF-β1 production from human keratinocytes by UVB," Exp Dermatol. 6. 105-110 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Jimbow K: "Semina's in Cutaneous Medicine and Surgery" W.B.Saunders Company(印刷中), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Jimbow K: "Dermatologic Clinics" W.B.Saunders Company(印刷中), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Salopek TG: "Multiple pigmented follicular cysts : a subtype of multiple pilosebaceous cysts." Br.J.Dermatol.134. 758-762 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toyofuku K: "Molecular chaperone,calnexin,associates with tyrosinase,the key enzyme in melanogenesis." Pigment Cell Res.5. 22-22 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chen H: "Involvement of phesphatidylinositol 3-kinase activity in the sorting and transport of newly synthesized tyrosinase-related protein-I in melanogenesis." Pigment Cell Res.5. 30-30 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Jibow K: "Molecular biology of tyrosinase-related protein ; assessment of biological role,biosynthesis and transport from TGN to prestage I melanosome through gene transfection." Pigment Cell Res.5. 34-35 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Gomez PF: "A small molecular weight,GTP-binding protein,rab-7 is involved in the transport of TRP-I from TGN to melanosomes." Pigment Cell Res.5. 35-36 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Park JS: "Target signal of TRP-I from Golgi to pre-stage I nelanosome,late endosome." Pigment Cell Res.5. 68-68 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kishi H: "Acral lentiginous melanema with the formation of giant tumors." Pigment Cell Res.5. 44-44 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Minamitsuji Y: "Selective apoptotic degeneration of follicular melanocytes by N-propiony1-4-5-cysteaminylphenol." Pigment Cell Res.5. 58-58 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Jimbow K: "Molecular biology of tyrosinase-related protein : Assessment of biological role biosynthesis and transport from TGN to stage I melanosome,late endosome,through gene transfection study." Pigment Cell Res.(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Jimbow K: "Melanogenesis and malignant melanoma : biochemistry,cell biology,molecular biology,pathophysiology diagnosis and treatment" Melanogenesis-based approaches for targeted chemotherapy and radiotherapy of melanoma., 13 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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