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Research on functional changes of motor unit by aging and reinnervation

Research Project

Project/Area Number 08407046
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionThe University of Tokyo

Principal Investigator

HARII Kiyonori  University of Tokyo, Faculty of Medicine, Professor, 医学部・附属病院, 教授 (50111539)

Co-Investigator(Kenkyū-buntansha) EGUCHI Tomoaki  University of Tokyo, Faculty of Medicine, Assistant, 医学部・附属病院, 助手 (00302688)
YOSHIMURA Kotaro  University of Tokyo, Faculty of Medicine, Lecturer, 医学部・附属病院, 講師 (60210762)
ASATO Hirotaka  University of Tokyo, Faculty of Medicine, Associate Professor, 医学部・附属病院, 助教授 (20222581)
Project Period (FY) 1996 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥34,200,000 (Direct Cost: ¥34,200,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1997: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1996: ¥22,800,000 (Direct Cost: ¥22,800,000)
Keywordsmyosin / apoptosis / embryonic myosin / aging / denervation / regeneration / atrophy / muscle / 再神経支配 / MyoD / 筋衛星細胞 / モーターユニット / 等尺性収縮力 / 長趾伸筋
Research Abstract

The purpose of this study is to examined the cellular and molecular events coincident with muscle denervation, especially a regenerative change seen following muscle denervation, the role of satellite cells involved in the process, and the possibility of apoptotic degeneration of myonuclei as a mechanism of myonuclei loss during muscle denervation atrophy. Myosin heavy chain (MHC) isoform expression during muscle denervation was examined using pyrophosphate acrylamide gel electrophoresis and immunohistochemistry. DNA fragmentation (apoptosis) in myonuclei of denervated fibers was investigated using agarose gel electrophoresis, the TUNEL technique and ELISA. Immunohistchemistry for MyoD and BrdU, and an ultrastructural survey were also performed. Following muscle denervation, embroyonic MHC which is not expressed in adult healthy muscles was expressed in some denervated fibers as well as small activated satellite cells ; maximal expression was observed two to three weeks after denervation. Activation and proliferation of satellite cells were observed, while few typical regenerating fibers were identified, suggesting that activated satellite cells fused to the denervated maternal fibers to try to repair them instead of fusing to each other to form new fibers, likely as a mechanism to compensate for atrophic changes after denervation. DNA fragmentation was detected by the TUNEL technique and ELISA, although DNA ladder formation was not observed with agarose gel electrophoresis. Apoptotic degeneration of myonuclei of denervated fibers was not confirmed, but it may contribute to loss of myoneuclei associated with denervation atrophy and induce activation of satellite cells.

Report

(5 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Harri, K, et al: "Aging effect in skeletal muscle recovery after"Scand . J. Plait . Reconstr. Hand. Surg. 30. 89-98 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Harri, K, et al: "One-Stage tranfer of the latissimus dorsi muscle"Plast Reconstr. Surq. 102. 941-951 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshimura k, et al: "Myasin Heavy chain expression in skeletal muscle"J. Surg. Res. 75. 135-147 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshimura k, et al: "Immunohisto chemical study of clinically"J. Surg. Res. 79. 31-38 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Veda K, et al: "Nenrovascular free muscle transfer combined"Plast. Reconstr Surg. 101. 1765-1773 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshimura k, et al: "Aregenerative change during muscle adaptation"J. Surg. Res. 81. 139-146 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Harii K, Asato H, Ueda K: "Aging effects in skeletal muscle recovery after reinnervation."Scand J Plast Reconstr Hand Surg. 30. 89-98 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Harii K, Asato H, Yoshimura K, Sugawara Y, Nakatsuka T, Ueda K: "One-stage transfer of the latissimus dorsi muscle for reanimation of a paralyzed face : a new alternative."Plast Reconstr Surg. 102. 941-951 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshimura K, Kuzon WM, Harii K: "Myosin heavy chain expression in skeletal muscle autografts under neural or anueral condition."J Surg Res. 75. 135-147 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshimura K, Harii K, Asato H, Ueda K, Yamada A: "Immunohistochemical study of clinically transplanted muscles."J Surg Res. 79. 31-38 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ueda K, Harii K, Asato H, Yamada A: "Neurovascular free muscle transfer combined with cross-face nerve grafting for the treatment of facial paralysis in children."Plast Reconsts Surg. 101. 1765-1773 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoshimura K, Harii K: "A regenerative change during muscle adaptation to denervation in rats."J Surg Res. 81. 139-146 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ueda K, Harii K, Asato H, Yoshimura K, Yamada A: "Evaluation of muscle graft using facial nerve on the affected side as a motor source in the treatment of facial paralysis."Scand J Plast Reconstr Hand Surg. 33. 47-57 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Harri, K, et al.: "Aging effects in skeletal muscle recovery after-"Scard. J. Plast. Reconstr. Hand. Surg.. 30. 89-98 (1996)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hariri, K, et al.: "One-stage transfer of the latissimus dorsi muscle-"Plast. Reconstr. Surg.. 102. 941-951 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshimura K, et al.: "Myosin Heavy chain expression in skeletal muscle-"J. Surg. Res.. 75. 135-147 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshimusra K, et al.: "Immunohisto chemical study of clinically-"J. Surg. Res.. 79. 31-38 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Veda K, et al.: "Neurovascular free muscle transfer combined-"Plast. Reconstr. Surg.. 101. 1765-1773 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshimura K, et al.: "Aregenerative change during muscle adaptation"J. Surg. Res.. 81. 139-146 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshimura K, et al.: "A Regenerative Change during muscle adaptation to denervation in Rats." J.Surg.Res.81 (2). 139-146 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshimura K, et al.: "Immunohistochemical Analysis of Clinically transplanted muscles." J.Surg.Res.79 (1). 31-38 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshimura K, et al.: "Myosin heavy chain expression inskeletal muscle autografts under neural or anearal condition." J.Surg.Res.75 (2). 135-147 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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