| Project/Area Number |
08407053
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Osaka University |
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Principal Investigator |
OKUYAMA Akihiko Osaka University Medical School, Professor, 医学部, 教授 (20093388)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMURA Masaya Osaka University Medical School, Assistant Professor, 医学部, 助手 (70273688)
MATSUMIYA Kiyomi Osaka University Medical School, Lecturer, 医学部, 講師 (90243237)
TAKAHARA Shiro Osaka University Medical School, Lecturer, 医学部, 講師 (70179547)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥16,100,000 (Direct Cost: ¥16,100,000)
Fiscal Year 1997: ¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 1996: ¥8,200,000 (Direct Cost: ¥8,200,000)
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| Keywords | HGF / Cytokine / Renal transplantation / Prostatic cancer / 慢性拒絶反応 / ラットモデル / マトリゲル / 腎虚血 |
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| Research Abstract |
HGF has been a focus of many researches as a multifunctional cytokine. We attained completely satisfactory results in 1997 and 1998, leading the world's researches of HGF concerning the following organs.
1. Kidney
Firstly, we showed the increased level of HGF during clinical rejection (Transpl Int), and proved preventive effect against renal toxicity of immunosuppressant. (Transpl Pro). Trying to see its long-term effect, we injected HGF intravenously for 4 weeks after renal transplantation in the rats. Surprisingly, 46% rats in the control group lost the function of the graft after 8 months, while in HGF group, all grafts survived functionally. Now we don't have any therapy for chronic rejection in renal transplantation. This study might give us a clue for using a cytokine to treat it.
2. Prostatic cancer cell lines
We studied the effects of HGF to invasive potential of three prostatic cancer cell lines. Two of them had c-MET,HGF receptor and only DU145 had responsiveness to HGF (Int J Urol). Furthermore, culture sup of fibroblast also could induce invasive potential of DU145 and it was inhibited by antibodies to HGF.Prostatic cancer is well known for a unique stromal-epitherial interaction and on-going experiments using different stromal cells might give us a clue to its understanding.
3. Male reproductive organs
We tried immunohistochemical study of HGF but the details remained unclear probably due to the level of expression.
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