| Project/Area Number |
08408033
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Osaka University |
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Principal Investigator |
HAMADA Hiroshi Institute for Molecular and Cellular Biology, Osaka University, 細胞生体工学センター, 教授 (00208589)
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| Co-Investigator(Kenkyū-buntansha) |
SAIJHO Yukio Institute for Molecular and Cellular Biology, Osaka University, 細胞生体工学センター, 助手 (20260333)
FUJII Hideta Institute for Molecular and Cellular Biology, Osaka University, 細胞生体工学センター, 助手 (10273723)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥29,200,000 (Direct Cost: ¥29,200,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1996: ¥23,300,000 (Direct Cost: ¥23,300,000)
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| Keywords | Left-right asymmetry / Lefty / transcriptional regulation |
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| Research Abstract |
Both lefty-1 and lefty-2 genes are expressed on the left side of developing mouse embryos and are implicated in left-right (L-R) axis formation. With the use of transgenic analysis, the transcriptional regulatory regions of these genes responsible for their L-R asymmetric expression have now been investigated. The 9.5-kb upstream region of lefty-1 and the 5.5-kb upstream region of lefty-2 reproduced the expression pattern of the corresponding gene. Examination of deletion constructs revealed the presence of a left side-specific enhancer (ASE) that is essential and sufficient for lefty-2 asymmetric expression. In contrast, the asymmetric expression of lefty-1 was shown to be determined by a combination of bilateral enhancers and a right side-specific silencer (RSS). The 9.5-kb region of lefty-1 and the 5.5-kb region of lefty-2 responded to iv and inv, upstream genes of lefty-1 and lefty-2. The regulation of lefty-2 by iv and inv was mediated by ASE. These results suggest that, in spite of the similarities between lefty-1 and lefty-2, different regulatory mechanisms underlie their asymmetric expression.
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