| Project/Area Number |
08408039
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ANDO Joji Dept.of Biomedical Engineering, Graduate School of Medicine, The University of Tokyo, Associate Professor, 大学院・医学系研究科, 助教授 (20159528)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Akira Dept.of Biomedical Engineering, Graduate School of Medicine, The University of T, 大学院・医学系研究科, 教授 (50014072)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥21,500,000 (Direct Cost: ¥21,500,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥17,600,000 (Direct Cost: ¥17,600,000)
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| Keywords | shear stress / endothelial cell / mRNA / differential display / G-protein / gene / 内皮細胞 / 壁ずり応力 / 血行力学因子 |
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| Research Abstract |
The purpose of this study was to investigate endothelial gene which were responsive to shear stress generated by blood flow and to find genes encoding new bio-active substances. First, in order to evaluate whole number of shear stress-responsive genes in endothelial cells, differential display of mRNAs was performed. The results showed that 33 of 15O7mRNAs detected were up-regulated by a shear stress of 15 dynes/cm^2 for 6 h, while 27 were down-regulated, indicating that around 4% of the whole endothelial genes are shear-sensitive. Second, 16 genes were cloned from these 60 shear-sensitive genes and sequenced. Homology search revealed 6 known genes encoding laminin B1 chain, H^+-ATP synthase coupling factor 6, lysyl oxidase, myosin light chain kinase, NADH dehydrogenase, and interleukin-8 receptor and 10 unknown genes. Lastly, we constructed a cDNA library from human umbilical vein endothelial cells which had been exposed to a shear stress of 15 dynes/cm^2 for 8 h, and looked for new G-protein coupled receptors. As a result we obtained a new one which we named PEG-1 (Flow-induced Endothelial G-protein-coupled receptor. The receptor consists of 375 amino acids and has a homology to angiotensin receptors or chemokine receptors.
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