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液-液界面におけるイオン選択的電荷分離の基礎とその多原子イオン検出法への応用

Research Project

Project/Area Number 08454233
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 分離・精製・検出法
Research InstitutionThe University of Tokyo

Principal Investigator

梅澤 喜夫  東京大学, 大学院・理学系研究科, 教授 (80011724)

Co-Investigator(Kenkyū-buntansha) BUHLMANN Phi (PHILIPPE Buh)  東京大学, 大学院・理学系研究所, 助手 (20262149)
遠田 浩司  東京大学, 大学院・理学系研究科, 助手 (60212065)
菅原 正雄  東京大学, 大学院・理学系研究科, 助教授 (50002176)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1996: ¥5,900,000 (Direct Cost: ¥5,900,000)
Keywordsイオノフォア含有液膜 / 分子プローブ / レーザー光第二高調波発生法(SHG) / リン酸イオンリセプター / 塩化物イオン選択性電極 / グアノシンリセプター / 硫酸イオン選択性電極 / 多点水素結合 / 相補的塩基対形成 / ヌクレオチド / 塩化物イオン選択性液膜 / 膜中脂溶性イオン / レーザー光第二高調波発生(SHG)法 / 膜電位分子プローブ
Research Abstract

1.イオノフォア含有液膜の界面電位と界面電荷密度の関係を定量的に調べる方法として,光応答性イオノフォアを分子プローブとして利用することを提案し,光で誘起された膜電位の絶対値及び電位応答勾配の変化量を,拡散電気二重層に基づく界面モデルを用いて定量的に説明した.(Anal.Chem.,69,3360-3369(1997))
2.レーザー光第二高調波発生法(SHG)を用いて,液膜中の脂溶性イオンの役割を調べた.脂溶性イオンを含まない液膜は目的イオンに対して電位応答もSHG応答も示さず,膜界面の電荷分離の大きさが水溶液中の目的イオン濃度が変化しても変わらないことを明らかにした.(Anal.Chem.,69,1919-1924(1997))
3.リジッドなキサンテン骨格と二つのチオウレア部位をもつレセプターを合成した.このレセプターはリン酸イオンと選択的に錯体を形成し,その錯体安定度定数はこれまで報告されている電荷中性のリン酸イオンレセプターの中で最大である.このキサンテン骨格をもつビスチオウレアレセプターに基づくイオン選択性電極は,血清中の塩化物イオンの定量が可能である.(Anal.Chem.,69,1038-1044(1997)),Tetrahedron,53,1647-1654(1997))
4.グアノシンヌクレオチドと5本の水素結合を介して結合する水素結合レセプターを開発した.この水素結合レセプターに基づく液膜はグアノシンヌクレオチドとアデノシンヌクレオチドを電位応答識別できる.(Anal.Chem.Acta,341,129-139(1997))
5.m-キシレン骨格をもつビスチオウレアレセプターを開発した.このレセプターに基づく液膜は硫酸イオンに対し10^<-6>M-10^<-2>Mの濃度範囲で電位応答を示し,硫酸イオンに対する選択性はこれまで報告された硫酸イオン選択性電極の中で最も優れている.(Anal.Chim.Acta,358,35-44(1998))

Report

(2 results)
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Nishizawa et al.: "Application of a Bis-Thiourea Ionophore for and Anion Selective Electrode with a Remarkable Sulfate Selectivity." Anal.Chem.Acta. 358. 35-44 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tohda et al.: "Photoswitchable Azobis (benzo-15-crown-5) Derivatives as a Molecular Probe for Phase Boundary Potentials at Ion-Selective Poly (vinyl chloride) Liquid Membranes." Anal.Chem. 69. 3360-3369 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Amemiya et al.: "Hydrogen Bond Based Recognition of Nucleotides by Neutral-Carrier Ion-Selective Electrodes." Anal.Chem.Acta. 341. 129-139 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yajima et al.: "Donnan Exclusion Failure of Neutral Ionophore-Based Ion-Selective Electrodes Studied by Optical Second-Harmonic Generation." Anal.Chem.69. 1919-1924 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Xiao et al.: "A Chloride Ion-Selective Solvent Polymeric Membrane Electrode based on a Hydrogen Bond Forming Ionophore." Anal.Chem.69. 1038-1044 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Buhlmann et al.: "Strong Hydrogen Bond-Mediated Complexation of H_2PO_4 by Neutral Bis-Thiourea Hosts." Tetrahedron. 53. 1647-1654 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] P.Buhlmann,et al.: "Strong Hydrogen Bond-Mediated Complexation of H_2PO_4 by Neutral Bis-Thiourea Hosts." Tetrahedron. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] K.P.Xiao,et al.: "A Chloride Ion-Selective Solvent Polymeric Membrane Electrode based on a Hydrogen Bond Forming Ionophore." Amal.Chem.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Sugawara, et al.: "Electrochemical Evaluation of Chemical Selectivity of Glutamate Receptor Ion Channel Proteins with a Multi-Channel Sensor." Biosensor & Bioelectonics. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Amemiya,et al.: "Hydrogen Bond Based Recognition of Nucleotides by Neutral-Carrier Ion-Selective Electrodes." Anala.Chim.Acta.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Tohda,et al.: "Ion Channel Sensor for Guanosine Nucleotides Based on Complementary Base Pairing." Isr.J.Chem.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Tohda,et al.: "Ion-Selective Charge Separation at the Phase Boundary of Ionophore-Incorporated Liquid Membranes as Studied by Optical Second Harmonic Generation." J.Mol.Struct.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Iitaka,et al.: "Orthophosphate Ion Sensors Based on a Quartz Crystal Microbalance Coated with Insoluble Orthophosphate Salts." Anal.Chim.Acta.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Sugawara,et al.: "Isolated Receptor Biosensors." Methods in Molecular Biology,Humana Press. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Radecka et al.: "Immuno Biosensors Based on Ion-Selective Electrodes." Methods in Molecular Biology,Humana Press. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Sugawara,et al.: "Biomembrane Mimetic Sensors." Current Topics in Biophysics,ed by P.T.Frangopol. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Sugawara,et al.: "Receptor Based Chemical Sensing." Frontiers in Biosensorics,I,ed.by F.D.Sheller. 121-131 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Sugawara,et al.: "Concentration of Dopamine by Proton-Driven Uphill Transport Using Lasalocid A as the Carrier." Anal.Sci.12. 331-335 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Yagi,et al.: "Channel Mimetic Sensing Membranes for Alkali Metal Cations Based on Oriented Monolayers of Calixarene Esters." J.Electroanal.Chem.401. 65-79 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 遠田浩司: "イオン選択性液膜界面における電位発生機構の分子レベルでの解釈" 分析化学. 45. 641-657 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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