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Regulation of cellular functions by the ubiquitin-proteasome system in Saccharomyces cerevisiae

Research Project

Project/Area Number 08454242
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 遺伝
Research InstitutionDepartment of Biological Sciences, Graduate School of Science, University of Tokyo

Principal Investigator

TOH-E Akio  University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (90029249)

Co-Investigator(Kenkyū-buntansha) KIKUCHI Yoshiko  Graduate School of Science, University of Tokyo, Associate Professor, 大学院・理学系研究科, 助教授 (00138124)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1996: ¥5,100,000 (Direct Cost: ¥5,100,000)
Keywordsproteasome / ubiquitin / cell cycle / proteolysis / Cdk / yeast / ユビキチン / SUN1 / SUN2
Research Abstract

Nin1P,a component of the 26S proteasome of Saccharomyces cerevisiae, is required for activation of Cdc28p kinase at the G1/S and G2/M boundaries. By exploiting the temperature sensitive phenotype of the nin1-1 mutant, we have screened for genes encoding proteins with related functions to Nin1P and have cloned and characterized two new multicopy suppressors, SUN1 and SUN2, of the nin1-1 mutation. SUN1 can suppress a null nin1 mutation, whereas SUN2, an essential gene, does not. Sun1p is a 268 amino acid protein which shows strong similarity to MBP1 of Arabidopsis thaliana, a homolog of the S5a subunit of the human 26S proteasome. Sun1p binds ubiquitin-lysozyme conjugates as do S5a and MBP1. Sun2p (523 amino acids) was found to be homologous to the p58 subunit of the human 26S proteasome. cDNA encoding the p58 component was cloned. Furthermore, expression of a derivative of p58 from which the N-terminal 150 amino acids had been removed restored the function of a null allele of SUN2. During glycerol density gradient centrifugation, both Sun1p and Sun2p comigrated with the known proteasome components. These results, as well as, other structural and functional studies, insicate that both Sun1p and Sun2p are components of the regulatory module of the yeast 26S proteasome

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] J.Imai, Toh-e and Y.Matsui: "Genetic analysis of the Saccharomyces cerevisiac RHO3 gene,encoding a Rho-type small GPTase,provides evidence for role in bud formation." Genetics. 142. 359-369 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] D.Mack, K.Nishimura, B.K.Dennehey et al.: "Identification of the budemergence gene BEM4 and its interactions with Rho-type GTPases in Saccharomyces cerevisiae" Mol.Cell.Biol.16. 4387-4395 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Matsui, R.Matsui, R.Akada and A.Toh-e: "Yeast src homology region 3 domain-binding proteins involved in bud formation." J.Cell.Biol.133. 865-878 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Yashiroda, T.Oguchi, Y.Yasuda, A.Toh-e and Y.Kikuchi: "Bull,a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae." Mol.Cell.Biol.16. 3255-3263 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Shirayama, Y.Matsui and A.Toh-e: "Dominant mutant alleles of yeast protein kinase gene CDC15 suppress the Ite1 defect in termination of M phase and genetically interact with CDC14." Mol.Gen.Genet.251. 176-185 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kawamura, K.Kominami, J.Takeuchi and A.Toh-e: "A multicopy suppressor of nin1-1 of the yeast Saccharomyces cerevisiae is a couterpart of the Drosophila melanogaster diphenol oxidase A2 gene,Dox-A2." Mol.Gen.Genet.251. 146-152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Tsurumi, Y.Shimizu, M.Saeki, S.Kato: "cDNA cloning and functional ananlysis of p97 subunit of the 26S proteasome,a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11." Eur.J.Biochem.239. 912-921 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yokota, S.Kagawa, Y.Shimizu et al.: "cDNA cloning of p112,the largest regulatory subunit of the human 26S proteasome,and functional analysis of its yeast homologue,Sen3p." Mol.Biol.Cell.7. 853-870 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Kominami, N.Okura, M.Kawamura et al.: "Yeast counterparts of subunits S5a and p58(S3)of the human 26S Proteasome are encoded by two multicopy suppressors of nin1-1." Mol.Biol.Cell.8. 171-187 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Uesono, A.Toh-e and Y.Kikuchi: "Ssd1p of Saccharomyces cerevisiae associates with RNA." J.Biol.Chem.272. 16103-16109 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Ito, Y.Matsui, A.Toh-e, T.Harashima and H.Inoue: "Isolation and characterization of the krev-1 gene,a novel member of ras superfamily in Neurospora crassa:involvement in sexual cycle progression." Mol.Gen.Genet.255. 429-437 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kagami, A.Toh-e and Y.Matsui: "SRO9,a multicopy suppressor of the bud growth defect in the Saccharomyces cerevisiae ρ3-deficient cells,shows strong genetic interactions with tropomyosin genes,suggesting its role in organization of the actin cytoskeleton." Genetics. 147. 1003-1016 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Andoh, T.Kato, Y.Matsui and A.Toh-e: "Phosphoinositide-specific phospholipase C forms a complex with 14-3-3 proteins and is involved in expression of UV-resistance in fission yeast." Mol.Gen.Genet.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Fujimuro, K.Tanaka, H.Yokosawa and A.Toh-e: "Son1p is a component of the 26S proteasome of the yeast Saccharomyces cerevisiae" FEBS Lett.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ito,S., Matsui,Y., Tuh-e,A., Hirashima,T., Inoue,H: "Isolation and cbaractenzrtion of the krev-1 gene,a novel member of ras superfamily in Newospura crassev : inwlvement in sexual cycle progreotion" Mol.Gen.Genet.255. 429-437 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Uesano,Y., Toh-e,A., Kikuchi,Y.: "Ssdl of Sacchenovrycls cerevisiae associates with RNA" J.Biol.Chem.272. 16103-16109 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kagami,M., Tuh-e,A., Matsui,Y.: "SRO9 a multicopy supprrnor f the bud growth defect in the Sacchevenyus cerevisinl Hiv3-Cficiuit cells ,shuces strmy genetic irteractions with tropomyosr geves,suggertory tis rble in organization of a chis Ug tusleeleter" Genetics. 147. 1003-1016 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Andoh,T., Kato,T.Jr., Matsui,Y., Tuh-e,A: "Phosphoinositide,specil-c phospholirase C forms a complex with 14-3-3 priteins and is in volved inexpression of UV resistance in fission yeast" Mol.Gen.Genef.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujimuro,M., Tanaka,K., Yukusawa,H., Tuh-e,A.: "Soulp is a compoment of the 265 prote ar me of the yeast faochawmyces cerevistoe" FEBS Lett.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Kominami,K.et al.: "Yeast counterparts of subnets S5a and p58(S3)of the human 26S proteasome are encoded by two multicopy supprersors of nin1-1" Mol.Biol.Cell. 8(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsurumi,C.et al.: "cDNA cloning and functional analysis of the p97 subunit of the 26S proteasome,a polypeptide identical to the type1 TNF receptor binding protein TRAP-2/55-11" Eur.J.Biochem. 239. 912-921 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshida,M.,Oguchi,T.,Yasuda,Y.,Toh-e,A.,and Kikuch,Y.: "Bul1,a new binding protein of the Rsp5-ubiquitin Qigase in Sacchanomyces cerevisiae" Mol.Biol.Cell. 16. 3255-3263 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yokota,K.,et al.: "cDNA cloning of p112,the largest regulatory subunit of the humar 26S proteasome,and funetional analysis of it yeast homolog,Sen3p" Mol.Biol.Cell. 7. 853-870 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kawamura,M.,Kominami,K.,Takeuchi,J.,Toh-e,A.: "A multropy suppressor of nin1-1 of the yeast Saccharitnyces cercuisiae is a counterpart of the Drosoplila welanogaster diphenol oxidage A_2 gene,DoxA_2" Mol.Gen.Genet. 251. 146-152 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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