| Project/Area Number |
08454261
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Okazaki National Research Institutes |
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Principal Investigator |
IIDA Shigeru National Institute for Basic Biology, Professor, 基礎生物学研究所, 教授 (30012777)
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| Co-Investigator(Kenkyū-buntansha) |
HABU Yoshiki National Institute for Basic Biology, Research Associate, 基礎生物学研究所, 助手 (80266915)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | transposable element / mutable allele / flower variegation / anthocyanin pigmentation / anthocyanin biosynthesis gene / the Japanese morning glory / the common moring glory / transgenic rice plant / 高等植物 / 転移 / トランスジェニックイネ |
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| Research Abstract |
The Japanese Morning glory (Pharbitis nil or Ipomoea nil) is a traditional horticultural plant in Japan, and extensive physiological and geneticalstudies on the plant have been conducted. Among several mutable loci that condition variegated flower phenotypes, two mutable alleles, flecked and speckled have been chosenfor molecular elucidation of the variegated phenotypes. The flecked mutant bears white floweres with colored flecks and sectors, and the flecked mutation was found to be caused by integration of the transposable element, Tpn1, into the DFR gene for anthocyanin pigmentation. We have also found that the seckled mutant also carries another transposable element, Tpn2, in the CHI gene for flower Pigmentation.
The mutable flaked line of the common morning glory (Pharbitis purpurea or Ipomea purpurea) also bears white flowers with colored flakes and sectors, and the mutable flaked allele is shown to be a newly identified CHS gene having a new transposable element, Tip100, inserted.
To understand the behavior of the transposable elements in heterologous system using transgenic plants, we are also characterizing somatic transposition of a non-autonomous Ds element mediated by the active Ac transposase in transgenic rice plants. We are currently investigating on the target selectivity in the somatic transposition of the Ds element.
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