| Project/Area Number |
08455095
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fluid engineering
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| Research Institution | Keio University |
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Principal Investigator |
TANISHITA Kazuo Keio University, Faculty Sci.Tech., Professor, 理工学部, 教授 (10101776)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Hiroyuki Keio University, Medical School, , Research Associate, 医学部, 助手 (90182550)
MATSUMURA Shinji Hamamatsu Medical College, Research Associate, 助手 (70276393)
IKEDA Mariko Keio University, Dept.of Biology, Professor, 文学部・生物学教室, 教授 (00051368)
OKA Kotaro Keio University, Faculty Sci.Tech., Assistant Professor, 理工学部, 講師 (10276412)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | endothelial cells / microscopic blood flow / remodeling / uptake of substance / mechanorecepter / mechanical adaptation / shear stress / バイオメカニクス / 内皮細胞 / 能動制御 / 生物流体力学 / 壁せん断応力 |
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| Research Abstract |
The endothlial cells on the inner surface of arterial vessel have various functions such as the secretion and uptake due to the external mechanical stimulus in the microscopic level. So the present study dealt with the microblood flow and the uptake of macromolecules in the endothelial cells which were obserced by the confocal laser microscopy and the corelation between the microblood flow and the uptake of macromolecules is to be revealed. In this study the endothelial cells obtained from the aortic vessel of pig is employed. After 48 hour exposure to shear stress, the endothelial cell layr on coverslips were incubated at 37C for 60 minutes in PBS containing tetramethylrhodamine isothiocyanate conjugate albumin (TRITC-albumin). Thereafter the uptake of albumin and the shape of endothelial cells were observed by a intercellular region. The albumin uptake depends on imposed shear stress. At 10 dyn/cm2, the albumin uptake showed a 1.3 folds increase. The albumin uptake decrease with increasing shear stress and the minimum uptake is quater of the control value at 60 dyn/cm2. This shear dependence of uptake is an unique feature of the cell and may play a key role for the controlling mchanism of endothelial cells.
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