| Project/Area Number |
08455383
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Osaka University |
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Principal Investigator |
YAMADA Yasuhiro Department of Biotechnology, Graduate School of Engineering, Osaka University.Professor, 大学院・工学研究科, 教授 (00011891)
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| Co-Investigator(Kenkyū-buntansha) |
NIHIRA Takuya Department of Biotechnology, Graduate School of Engineering, Osaka University.As, 大学院・工学研究科, 助教授 (70144441)
IHARA Fumio Department of Biotechnology, Graduate School of Engineering, Osaka University.As (70252583)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Streptomyces hormones / Streptomyces coelicolor / structure determination / gamma-butyrolactone autoregulator / γ-ブチロラクトン / オートレギュレーター / Streptomyces coelicolor A3(2) / 構造決定 / 内生ホルモン / 形態分化 |
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| Research Abstract |
Streptomycetes are Gram-positive soil bacteria characterized by their ability to produce numerous kinds of useful secondary metabolites as well as by their complex life cycle including the formation of aerial mycelia and spores. The two characteristic phenotypes have been know to link each other, and low-molecular-weight compounds called gamma-butyrolactone autoregulators have been found in some Streptomyces species to regulate the two phenotypes. However, with the most genetically studied among Streptomyces species, Streptomyces coelicolor A3(2), nothing has been know on the nature of the intrinsic hormones to regulate the antibiotic production or morphological differentiation.
1. S.coelicolor A3(2) was studied intensively whether it has low-molecular-weight compounds to regulate the antibiotic production or the morphological differentiation.
2. S.coelicolor A3(2) was found to have a compound named Scb1 which induces the production of two antibiotics, actinorhodin and undecylprodigiosin.
3. From 500-liters culture broth, 320 mug of pure Scb1 was purified.
4. Plain structure of Scb1 was estimated from 600 MHz H-NMR and MS analyses.
5. Chiral synthetic routes for four possible Scb1 stereoisomers were established, and comparison of natural Scb1 with the four synthetic isomers resulted in the determination of Scb1 as (2R, 3R, 1'R)-2-(1'-hydroxy-6'-methylheptyl)-3-hydroxymethyl gamma-butanolide.
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