| Project/Area Number |
08455437
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
高分子合成
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| Research Institution | TOKYO INSTITUTE OF TECHNOLOGY |
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Principal Investigator |
OKAHATA Yoshio Tokyo Institute of Technology, Department of Biomolecular Engineering, Professor, 生命理工学部, 教授 (80038017)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Toshiaki Tokyo Institute of Technology, Department of Biomolecular Engineering, Research, 生命理工学部, 助手 (50262308)
EBARA Yasuhito Tokyo Institute of Technology, Department of Biomolecular Engineering, Research, 生命理工学部, 助手 (40251657)
SATO Toshinori Tokyo Institute of Technology, Department of Biomolecular Engineering, Associate, 生命理工学部, 助教授 (00162454)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1996: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | DNA / Langmuir-Blodgett Film / Aligned thin film / Cast Film / DNA-lipid complex |
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| Research Abstract |
We have prepared DNA-lipid complex by mixing both aqueous solutions of DNA from sermon testes and cationic lipid molecules. The precipitated DNA-lipid complex is soluble in most of organic media, bur not in aqueous solutions. When chloroform solution of DNA-lipid complex was cast on a glass plate, a transparent, physically strong film was obtained. Following results were obtained.
1)A cast film was stretched in one direction, the DNA-aligned film was obtained, which was confirmed by X-rya diffraction analyses.
2)When the DNA-lipid cast film was soaked in aqueous solution of ethidium bromide, the film was colored to red due to the intercalated ethidium bromide dye molecules. Orientation of intercalated dye molecules could be confirmed by polarized absorption spectra of the film.
3)DNA-aligned cast film showed a large anisotropic behavior of conductivity. The electrostatic conductivity was observed along the DNA strands in the film, but not perpendicular to the DNA strands.
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