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Biosynthesis and Properties Control of Bacterial Cellulose

Research Project

Project/Area Number 08455445
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 高分子構造・物性(含繊維)
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TAKAI Mitsuo  Hokkaido Univ., Grad.School of Eng., Prof., 大学院・工学研究科, 教授 (50002019)

Co-Investigator(Kenkyū-buntansha) TAJIMA Kenji  Grad.School of Eng., Ass., 大学院・工学研究科, 助手 (00271643)
FUJIWARA Masashi  Grad.School of Eng., Ass., 大学院・工学研究科, 助手 (30229075)
SHIBA Toshikazu  Grad.School of Eng., Assoc.Prof., 大学院・工学研究科, 助教授 (60241303)
ERATA Tomoki  Grad.School of Eng., Assoc.Prof., 大学院・工学研究科, 助教授 (30213581)
MUNEKATA Masanobu  Grad.School of Eng., Prof., 大学院・工学研究科, 教授 (50261326)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsBacterial Cellulose (BC) / Water-soluble Polymer (WSP) / BC Composite (BCC) / Increasing Yield / Biodegradability Control / Bioreactor with rotating-disk / Vibratory Young's Modulus / 培地成分 / 酵母エキス / アミノ酸 / pH / グルコン酸
Research Abstract

Bacterial cellulose (BC) productivity has been enhanced by the presence of 3.5% concentration of yeast extract in the medium as high as twice of the standard culture(0.5% yeast extract). Cell growth increased markedly with an increase in yeast extract concentration. pH of the medium decreased from the initial 5.9 to the range of 3.5-4.5 during 2 days incubation, however, after the prolonged incubation gradual increasing in pH was observed. Glucose as a carbon source was nevertheless consumed thoroughly during 4 days incubation, cellulose production proceeded slowly as long as 15 days incubation. An excess of yeast extract in the medium appear to play an role in the activator for cellulose biosynthesis. Bacterial cellulose (NBC) produced by Acetobacter xylinum (A.xylinum) has both great mechanical strength and biodegradability.The properties were controlled by the incorporation of water-soluble polymers (WSP) such as carboxymethyl cellulose (CMC), methyl cellulose (MC) and polyethylene … More glycol (PEG). Small amounts of the WSPs were added to the standerd Hestrin-Schramm medium.The WSPs were incorporated into BC during incubation. The cellulose composite (BCC) will be a kind of interpenetrating polymer.The BCC yield by weight were 1.5 times for MC and 1.8 times for CMC as compared with the weight of NBC control. The contents of MC and CMC in the BCC were checked by elemental analysis of C%, 45% for CMC and 20% for MC,respectively. From the X-ray analysis, the diffraction of (110) appeared sharply as a resuli of the disorientation of (110). The WSPs influence the preferential orientation of BC.Young's modulus of the BCC was about 1-3 times that of NBC (30-40GPa). BCC (CMC) was degraded completely by cellulase for 6 hr, while only 20-40% of BCC (MC) was degraded for 48hr. For degadation test in native soil, all BCC samples were degaded considerably at 30゚C for 4 weeks as well as NBC.There is some possibility to control a biodegadability of BC.The method of BCC production will be used to synthesize a new type of BC having both excellent biodegradability and other functions. The most effective incubation methods for NBC production were examined in various mode of bioreactor, and then on the basis of the results, the bioreactor with rotating-disk has been designed. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] K, Tajima et al.: "Synthesis of two water-soluble polysaccharide by Acetobacter sp.NCI1005" Macromol.Symp.120. 19-28 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 惠良田 知樹 他: "天然セルロースの13C CP/MAS NMRスペクトルの完全帰属" Cellulose communications. 4(3). 128-131 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K, Tajima et al.: "Enhancement of Bacterial Cellulose Productivity by Water-soluble Chitosan" Mokuzai Gakkaishi. 42(3). 279-288 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K, Tajima et al.: "Enhancement of Bacterial Cellulos Productivity and Preparation of Branched Polysaccharide-Bacteriai Cellulos Composite by Co-cultivation of Acetobacter Species" Sen-i Gakkaishi. 51(7). 323-332 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K, Tajima et al.: "Biological Control of Cellulose" Macromol.Symp.99. 149-155 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K, Tajima et al.: "Synthesis of Bacterial Cellulose Composite(BCC)by Acetobacter xylinuum,I" Mokuzai Gakkaishi. 41(8). 749-757 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T, Nakamura et al.分担執筆: "Cellulose and Cellulose Derivatives:Physicochemical Aspects and Industrial Application,Cellulose Production by Acetobacter xylinum in the Presence of Cellulase" published by Ellis Horwood, 3-8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高井 光男, 分担執筆: "生分解性プラスチックハンドブック,第2編第2章第2節微生物セルロースの構造・物性・機能" 出版社:NTS, 221-227 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Tajima, N.Uenishi, M.Fujiwara, T.Erata, M.Munekata, M.Takai: "Synthesis of two water-soluble polysaccharide by Acetobacter sp.NC11005" Macromol.Symp.120. 19-28 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Erata, T.Shikano, S.Yunoki and M.Taki: "The complete assignment of the 13C CP/MAS NMR sperctrum of native cellulose by using 13C labeled glucose" Cellulose Communications. 4, No.3. 128-131 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Tajima, M.Fujiwara, M.Takai and J.Hayashi: "Enhancement of Bacterial Cellulose Productivity by Water-Soluble Chitosan" Mokuzai Gakkaishi. 42(3). 279-288 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Tajima, H.Itoh, M.Fujiwara, M.Takai, J.Hayashi: "Enhancement of bacterial Cellulose productivity and preparation of branched polysaccharide-bacterial cellulose composite by cocultivation of Acetobacter species." Sen-i Ggakkaish. 51. 323-332 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Tajima, M.Fujiwara, M.Takai: "Biological Control of Cellulose" Macromol.Symp.99. 149-155 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Tajima, M.Fujiwara, M.Takai, J.Hayashi: "Synthesis of bacterial cellulose composite by Acetobacter xylinum I-Its mechanical strength and biodegradability-." Mokuzai Gakkaishi. 41. 749-757 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nakamura, K.Tajima, M.Fujiwara, M.Takai, J.Hayashi: Cellulose production by Acetobacter xylinum in the presence of cellulase., in"Cellulose and cellulose derivatives : physico-chemical aspects and industrial applications". Ellis Horwood, Chichester, 3-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Takai: (Part2, Chapter2, Passage2) Struetures, properties and functions of bacterial cellulose, in"Biodegradable plastics Handbook". NTS, 221-227 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Tajima, et al.: "Synthesis of tow water-soluble polysaccharide by Acetobacter sp.NCI1005" Macromol.Symp.120. 19-28 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 恵良田 知樹, 他: "天然セルロースの13C CP/MAS NMRスペクトルの完全帰属" Cellulose communications. 4(3). 128-131 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Tajima et al.: "Enhancement of Bacterial Cellulose Productivity by Water-soluble Chitosan" Mokuzai Gakkaishi. 42 (3). 279-288 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Tajima et al.: "Enhancement of Bacterial Cellulose Productivity and Preparation of Branched Polysaccharide-bacterial Cellulose Composite by Co-cultivation of Acetobacter Species" Sen-i Gakkaishi. 51 (7). 323-332 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Tajima et al.: "Biological Control of Cellulose" Macromol.Symp.99. 149-155 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Tajima et al.: "Synthesis of Bacterial Cellulose Composite (BCC) by Acetobacter xylinum, I" Mokuzai Gakkaishi. 41 (8). 749-757 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] J.-Y.Chen et al.: "Effect of Kraft Cooking Conditions on Efficiency of Oxygen Delignification, I." Mokuzai Gakkaishi. 41 (8). 757-766 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] J.-Y.Chen et al.: "A Method for Isolation of Miled-Wood Lignin involving Solvent swelling prior to Enzyme Treatment" Wood Science and Technology. 47. 235-240 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Nakamura et al.: "Cellulose Production by Acetobacter xylinum in the Presence of Cellulase" "Cellulose and Cellulose Derivatives : Physicochemical Aspects and Industrial Application" published by Ellis Horwood 分担執筆, 3-8 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高井 光男: "第2編第2章第2節 微生物セルロースの構造・物性・機能" 「生分解性プラスチックハンドブック」(出版社:NTS)分担執筆, 221-227 (1995)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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