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GENETIC AND BREEDING STUDIES ON AMYLOPECTIN MUTANTS IN RICE

Research Project

Project/Area Number 08456004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

SATOH Hikaru  KYUSHU UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (70128031)

Co-Investigator(Kenkyū-buntansha) KUMAMARU Toshihiro  KYUSHU UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 助教授 (00284555)
YOSHIMURA Atsushi  KYUSHU UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (00182816)
小川 雅広  山口県立大学, 家政学部, 助教授 (10160772)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsRICE / ENDOSPERM STARCH / AMYLOPECTIN / MUTANT / STARCH BIOSYNTHESIS ENZYME / BREEDING / GENE ANALYSIS / MNU TREATMENT / 枝作り酵素 / SDS-PAGE / 胚乳でんぷん / 枝つくり酵素 / MNU授精卵処理
Research Abstract

Amylopectin is a major constituent of starch, being about 80%, in rice and therefore, will be expected to affect greatly on the eating quality of cooked rice and the processing properties for industrial use. In spite of this importance, little attention has, so far, put on amylopectin in rice breeding, because of limited numbers of the information for related genes modifying amylopectin structure and of the genetic resources in amylopectin. In this research, we tried to induce the mutants for amylopectin by MNU treatment of fertilized egg cells in rice and to characterize the genetic and physicochemical properties for using them as breeding materials.
We developed and devised the speedy and simplified methods for the iodine colorimetric measurement of starch/iodine complex using the microprocessor controlled diode array spectrograph DU 7000 Spectrophotometer (Beckman), SDS-PAGE analysis of starch biosynthesis related enzyme proteins, Native-PAGE/Activity staining for amylopectin biosynt … More hesis related enzymes such as starch branching enzymes, starch debranching enzymes, starch synthase and pullanase, and the measurement of starch gelatinization using urea. Using these methods, we screened the mutant strains induced by MNU treatment of fertilized egg cells of three kinds of two Japonica rice cultivar, Kinmaze and Taichung 65, and a Indica rice cultivar IR36, and obtained various kinds of mutants modifying the amylopectin structure of endosperm starch, such as amylose extender (ae), floury 1(flo 1), floury 2(flo 2), floury 3(flo 3), floury 4(flo 4), sugary 1(sug 1) etc. The structural and physicochemical analyses of endosperm starches in these mutants shows that the changes in branching structure of amylopectin modified more greatly than the amylose content on the processing properties of starch such as the gelatinization or swelling of starch, which greatly affects the cooking quality of boiled rice and the industrial processing quality of rice starch. These facts indicates strongly the great importance of genetic resources for amylopectin in the breeding to improve the grain quality in rice. The results obtained in this research will play the important rolls not only as breeding materials improving the grain quality in rice but also as the materials of academic researches comprehending the genetic and biochemical regulation of amylopectin biosynthesis. Less

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takahashi A.,T.Kawasaki,K.Henmi,K.Shii,O.Kodama,H.Satoh and K.Shimamoto: "Coordinated activation of defence responses in lesion-mimic mutants of rice" Plant J.17(5). (in press) (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Umemoto T.:Nakamura Y.:Satoh H. and Terashima K.: "Differences in amylopectin structure between two rice varieties in relation to the effects of temperature during grain-filling" Starch. 51 in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoh H.,L.Q.Qu,T.Kumakura and M.Ogawa: "Gluterin mutants indeced by MNU treatment in rice" Rice Genet.Newsl.14. 81-84 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qu L.Q.,Satoh H.,T.Kumakura and M.Ogawa: "Genetic analyses of gluterin mutants in rice" Rice Genet.Newsl.14. 245-249 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Nakamura,T.Umemoto,Y.Takahata,K.Komae,E.Amano,H.Satah: "correlation between activities of starch debrancing enzynic(R-enxynic or pullulanse)and α-polygkucan structure in endosperms of sugary-1 mutants in of rice." Plant Journal. 116. 245-249 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kumakura,H.Sato and H.Satoh: "High-Lysine mutants of rice,Oryza sativa L" Plant Breeding. 12(1). 143-153 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Nakamura, T.Umemoto, Y.Takahata, K.Komae, E.Amano and H.Satoh: "Changes in structure of starch and enzyme activities affected by sugary mutations in developing rice endosperm. Possible role of starch debranching enzyme (R-enzyme) in amylopectin biosynthesis" Physiol.Plant.97. 491-498 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kumamaru, H.Sato and H.Satoh: "High lysine mutant of rice, Oryza sativa." Plant Breeding. 116. 245-249 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Nakamura, A.Kubo, T.Shimamune, T.Matsuda, K.Harada and H.Satoh: "Correlation between activities of starch debranching enzyme and alpha-polyglucan structure in endosperms of sugary-1 mutants of rice" The Plant Journal. 12 (1). 143-153 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoh, H.Chloroplast.: "In "Science of the Rice Plant, Vol.III Genetics", eds.T.Matuo, F.Kikuchi and H.Yamaguchi" NOBNKYO. 336-343 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Qu L.Q., Satoh H., T.Kumamaru and M.Ogawa: "Genetic analyses of gluterin mutants in rice" Rice Genet.Newsl.14. 78-81 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoh H., L.Q.Qu, T.Kumamaru and M.Ogawa: "Gluterin mutants indeced by MNU treatment in race" Rice Genet.Newsl.14. 81-84 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Umemoto T., Y.Nakamura, H.Satoh and K.Terashima: "Difference in amylopectin structure between two rice varieties in relation to the effects of temperature during grain-filling" Starch. 51 : (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] A.Kubo, N.Fujita, K.Harada, T.Matsuda, H.Satoh and Y.Nakamura: "The starch debranching enzymes isoamylase and pullulanase are both involved in amylopectin biosynthesis in rice endosperm" Plant Physiol. (Submitted). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi A.,T.Kawasaki,K.Henmi,K.Shii,O.Kodama,H.Satoh and K.Shimamoto: "Coordinated activallon of defence responses in lesion-mimic mutants of rice" Plant J.17(5)(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Umemoto T.,Y.Nakamura,H.Satoh and K.Terashima: "Difference in amytopectin structure between two rice vartellom in rotation to the effects of temperature during grain-union" Starch. 51(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Satoh H.,L.Q.Qu,T.Kumamaru and M.Ogawa: "Gluterin mutants indeced by MNU treatment in rice" Rice Genet.Newsl.14(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Qu L.Q.,Satoh H.,T.Kumamaru and M.Ogawa: "Genetic analyses of gluterin mutants in rice" Rice Genet.Newsl.14(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 佐藤 光: "イネの成分育種における突然変異の利用" 放射線と産業. 73. 9-14 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nakamura,Y.,A.kubo,T.Shimamura,T.Matsuda,K.Harada and H.Satoh.: "Correlation between avtivities of starch debranching enzyme and α-polyglucan structure in endosperms of sugary-1 mutants in rice." Plant J.12. 143-153 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kumamaru,T.,H.Sato and H.Satoh.: "High-lysine mutants of rice,Oryza sativa L." Plant Breed.116. 245-249 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Satoh H.: "NOBNKYO 農文協" Chloroplst.In“Science of the Rice Plant,Vol.III Genetics",8(336-343) (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Nakamura,A.Kubo,T.Shimamune,T.Matsuda,K.Harada and H.Satoh: "Changes in Structure of starch and enzyme activities affected by sugary mutations in developing rice endosperm.Possible role of starch debranching enzyme(R-enzyme)in amylopectin biosynthesis" Physioogia Plantarum. 97. 491-498 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] N.Nagasawa,M.Miyoshi,H.Kitano,H.Satoh and Y.Nagato: "Mutations associated with floral organ number in rice" Planta. 198. 627-633 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Nakamura,T.Umemoto,Y.Takahata,K.Komae,E.Amano and H.Satah: "Correlation between activities of starch debranching enzyme(R-enzyme or pullulanase)and α-polyglucan structure in endosperms of sugary-1 mutants in of rice" Plant Journal. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Kumamura,H.Sato and H.Satoh: "High-Lysine mutants of rice,Oryza sativa L." Plant Breeding. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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