| Project/Area Number |
08456006
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
OKINO Hiroko Nat.Inst.Genet., Div.of Agric.Genetics, Professor, 総合遺伝研究系, 教授 (70000247)
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| Co-Investigator(Kenkyū-buntansha) |
CAI Hongwei Nat.Inst.Genet., Div.of Agric.Genetics, Assist.Professor, 総合遺伝研究系, 助手 (20290893)
春島 嘉章 (財)遺伝学普及会, 研究員
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1996: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | Domestication in rice / Quantitative trait loci / Molecular marker / Wild rice / Evolutionary genetics / Adaptive gene block / 野性イネ / 栽培化 / 遺伝子地図 / 遺伝子の多面発現 |
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| Research Abstract |
Wide and cultivated rices differ in many quantitative characters responsible for yielding capacity and propagation method. The objective of this study is to identify loci of those quantitative traits (QTL) and elucidate their network on rice genome using a cross between cultivated rice and a strain of wild rice.
1) QTL analysis
One-hundred forty molecular markers were found to be polymorphic between two parents. Segregation of those markers were surveyed among 125 R_6 Recombinant Inbred Lines (RILs) derived from the above cross, and linkage map was constructed. More than 30 quantitative traits were measured in respective RILs, and cosegregation of molecular markers and quantitative traits were analyzed by qGENE.A total of 176 QTLs for 22 traits were detected. QTLs controlling a given trait were widely scattered on different chromosomes. QTLs for different traits tended to cluster on several specific regions of 12 chromosomes. Such clusters were found near the markers which showed signifi
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cant excess of cultivar-derived alleles.
2) Selection experiment on seed shattering
Two hybrid populations of the above cross were established by propagating after F_3 in bulk as follows. One is shattering group (S) in which seeds shattered on the ground were collected and used for raising next generation. The other is non-shattering group (NS) in which seeds remaining on the panicles were harvested and used for raising next generation. Two F_7 populations thus established were examined regarding 80 RFLP markers and various quantitative traits. Lines of S group showed later heading, stronger seed dormancy, stronger regenerating ability than those of NS group. Allele frequencies of some RFLP showed significant difference between S and NS groups. They are mostly linked with QTLs responsible for seed shattering.
The above results suggest possible genetic mechanisms of domestication in rice. "Cultivation" defined by seeding and harvesting by man must have worked as strong natural selection favoring non-shattering plants. Further, linkage or gene cluster of QTLs also contributed to forming adaptive association of characters in domestication process. Less
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