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Specificity of Plant Infection : Molecular Mechanism of Determination of Basic Compatibility by Host-Specific Toxins

Research Project

Project/Area Number 08456028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionTottori University

Principal Investigator

OTANI Hiroshi  Tottori University, The United Graduate School of Agricultural Sciences, Professor, 大学院・連合農学研究科, 教授 (50032305)

Co-Investigator(Kenkyū-buntansha) KODAMA Motoichiro  Tottori University, Faculty of Agriculture Lecturer, 農学部, 講師 (00183343)
NAKAJIMA Hiromitsu  Tottori University, Faculty of Agriculture Associate Professor, 農学部, 助教授 (40144646)
KOHMOTO Keisuke  Tottori University, Faculty of Agriculture Professor, 農学部, 教授 (80032093)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1996: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordsbasic compatibility / host-specific toxin / host-specific toxin biosynthesis gene / plasma membrane / chloroplast / receptor / 環状ペプチド合成酵素遺伝子 / 毒素受容体 / AM毒素生合成遺伝子
Research Abstract

Certain fungal pathogens are known to produce host-specific toxins (HSTs) as determinants of basic compatibility in plant infection. There are now about twenty pathogens that produce HST.We found that Alternaria brassicicola, cucumber pathotype and tomoto pathotype of Corynespora cassiicola, and Stemphylium vesicarium produce new HSTs named AB-, CCC-, CCT-and SV-toxins, respectively. Unlike other HSTs which are low-molecular-weight secondary metabolites, AB-toxin was a protein and estimated to be 35 kDa.
A.brassicicola released AB-toxin from the germinating spores only on host leaves, indicating that AB-toxin production by the pathogen is induced by host-related factors. There are seven pathotypes in A.alternata pathogens. HST (AL-toxin) deficient mutants from tomato to pathotype using REMI method. On the other hand, PCR products were obtained from apple pathotypes, using primers designed from cyclic peptide synthase genes. Detailed molecular analysis of these mutants and PCR products a … More re now in progress. Electrophoreic karyotyping of A.alternata pathotypes were determined by PFGE.In all pathotypes, a chromosomal band with small size of <1.6 Mb was detected commonly. Southern analysis using PCR products from apple pathotype showed that AM-toxin synthesis gene exists in the small chromosome.
HSTs (AK-toxin and AM-toxin) produced by Japanese pear and apple pathotypes of A.alternata have primary effects on plasma membranes (PMs) and chloroplasts (Cs) of susceptible cultivars, respectively. Therefore, PMs and Cs were isolated from respective hosts, and interaction between HSTs and proteins in PMs or Cs was analyzed by using a biosensor. Results suggest that AK- and AM-toxin-binding proteins (receptors) exist in PMs and Cs of susceptible cultivars, respectively. Total proteins of apple leaves were analyzed by 2-D gel electrophoresis. A spot of 60 kDa protein with pI of 5.5 was detected only in susceptible cultivars. Isolation and characterization of these proteins is in progress. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Kodama, M.et al.: "Host-specific toxin deficient mutants of Alternaria alternata tomato pathotype by restriction enzyme-mediated integration." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto,K.and Yoder,O.C.),Kluwer Academic Publishers. 35-42 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh, Y.et al.: "A catalytic domain of a cyclic peptide synthetase that is specific for the apple pathotype of Alternaria alternata and its possible involvement in host-specific AM-toxin production." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto,K.and Yoder,O.C.),Kluwer Academic Publishers. 53-61 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Otani, H.et al.: "Involvement of host factors in the production of a protein host-specific toxin produced by Alternaria brassicicola." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto,K.and Yoder,O.C.),Kluwer Academic Publishers. 63-69 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tabira, H.et al.: "Analysis of protein associated with susceptibility to Alternaria blotch by use of resistant mutant in apple." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto,K.and Yoder,O.C.),Kluwer Academic Publishers. 387-397 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Otani, H.et al.: "Production of a host-specific toxin by germinating spores of Alternaria brassicicola." Physiological and Molecular Plant Pathology. 52 (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akamatsu, H.et al.: "AAL-toxin-deficient mutants of Alternaria alternata tomato pathotype by restriction enzyme-mediated integration." Phytopathology. 87. 967-972 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 児玉 基一朗 ら: "宿主特異的毒素:その生物学的側面.「植物病害の化学」(市原耿民・上野民夫編)" 学会出版センター(東京), 39-48 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kodama, M.et al.: "Host-specific toxin deficient mutants of Alternaria alternata tomato pathotype by restriction enzyme-mediated integration." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto, K.and Yoder, O.C.), Kluwer Academic Publishers. 35-42 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Itoh, Y.et al.: "A catalytic domain of a cyclic peptide synthetase that is specific for the apple pathotype of Alternaria alternata and its possible involvement in host-specific AM-toxin production." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto, K.and Yoder, O.C.), Kluwer Academic Publishers. 53-61 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Otani, H.et al.: "Involvement of host factors in the production of a protein host-specific toxin produced by Alternaria brassicicola." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto, K.and Yoder, O.C.), Kluwer Academic Publishers. 63-69 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tabira, H.et al.: "Analysis of protein associated with susceptibility to Alternaria blotch by use of resistant mutant in apple." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto, K.and Yoder, O.C.), Kluwer Academic Publishers. 387-397 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Otani, H., et al.: "Production of a host-specific toxin by germinating spores of Alternaria brassicicola." Physiological and Molecular Plant Pathology. 52 (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akamatsu, H.et al.: "AAL-toxin-deficient mutants of Alternaria alternata tomato pathotype by restriction enzyme-mediated integration" Phytopathology. 87. 967-972 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kodama, M.et al.: Host-specific toxin : The biological aspect. (in Japanese). Chemistry of Plant Disease (Ed.by Ichihara, A.and Ueno, T.), 39-48 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kodama,M.: "Host-specific toxin deficient mutants of Alternaria alternata tomato pathotype by restriction enzyme-mediated integration." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto,K.and Yoder,O.C.), Kluwer Academic Publishers. 35-42 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Itoh,Y.: "A catalytic domain of a cyclic peptide synthetase that is specific for the apple pathotype of Alternaria alternata and its possible involvement in host-specific AM-toxin production." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto,K.and Yoder,O.C.), Kluwer Academic Publishers. 53-61 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Otani,H.: "Involvement of host factors in the production of a protein host-specific toxin produced by Alternaria brassicicola." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto,K.and Yoder,O.C.), Kluwer Academic Publishers. 63-69 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tabira,H.: "Analysis of protein associated with susceptibility to Alternaria blotch by use of resistant mutant in apple." Molecular Genetics of Host-Specific Toxins in Plant Disease (Ed.by Kohmoto,K.and Yoder,O.C.), Kluwer Academic Publishers. 387-397 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Akamatsu,H.: "AAL-toxin-deficient mutants of Alternaria alternata tomato pathotype by restriction enzyme-mediated integration." Phytopathology. 87. 967-972 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Otani,H.: "Production of a host-specific toxin by germinating spores of Alternaria brassicicola." Physiological and Molecular Plant Pathology. (in Press) (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 児玉基一郎: "宿主特異的毒素:その生物学的側面.「植物病害の化学」(市原耿民・上野民夫編)" 学会出版センター(東京), 39-48 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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