| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Research Abstract |
(1) Gene sequence analysis revealed that the Cry proteins of the five Lepidoptera-specific Bacillus thuringiensis strains, that belong to the three H serovars (fukuokaensis, sotto, and galleriae) and produce spherical parasporal inclusions, are of the single category, a novel class belonging to the Cry9 delta-endotoxin.
(2) Of 1713 Japanese isolates of Bacillus thuringiensis, 609 (35.5%) exhibited high larvicidal activity against the diamondback moth, Plutella xylostella. Of these toxic isolates, 605 belonged to the H serotypes 1-10. When five selected highly toxic isolates, belonging to four H serovars (sumiyoshiensis, 2 isolates ; fukuokaensis, 1 ; canadensis 1 ; morrisoni, 1), were examined for their delta-endotoxins, the four isolates, excluding the morrisoni isolate, were assigned to the Cry9D-producing strains.
(3) Isolation experiments failed to recover Bacillus thuringiensis from soils and floral samples collected in the coastal area of the L_tzow-Holm Bay of the Antarctica sub-continent.
(4) Characterization of the microsporidians, isolated from diseased larvae of the silkworm (Bombyx mori) in Brazilian sericultural farms, revealed that (i) the isolates with large- to middle-sized spores belong to the genus Nosema, but differ from the species Nosema bombycis, and (ii) the isolates with small-sized spores belong to the genus Pleistophora.
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