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Regulatory mechanisms of xpression of glycosidase genes in filamentous fungi and their applications

Research Project

Project/Area Number 08456049
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

TSUKAGOSHI Norihiro  NAGOYA UNIVERSITY,DEPARTMENT OF AGRICULTURE PROFESSOR, 農学部, 教授 (50115599)

Co-Investigator(Kenkyū-buntansha) KATO Masashi  NAGOYA UNIVERSITY,DEPARTMENT OF AGRICULTURE,ASSISTANT PROFESSOR, 農学部, 助手 (70242849)
KOBAYASHI Tetsuo  NAGOYA UNIVERSITY,DEPARTMENT OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (20170334)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1996: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordsexpression properties of filamentous fingal genes / Aspergillus oryzae / Aspergillus nidulans / Chaetomium gracile / Taka-amylase A / cellulase / xylanase / CCAAT binding factor, AnCP / CDAAT
Research Abstract

Filamentous fungi represented by Aspergillus oryzae have been playd important roles in Japanese fermentation industries. We have examined the expression properties of Taka-amylase A gene isolated from A.oryzae using A.nidulans as an intermediate host. CCAAT sequence and AnCP,CCAAT binding protein, have been shown to play important roles in enhancement of Taka-amylase A gene expression under the inducible conditions.
In this project, cellulase and xylanase genes have been characterized to elucidate their regulatory mechanisms in addition to Taka-amylase gene in order to develop a novel way to utilize efficiently various reproductive natural resources, especially carbohydrates such as starch, cellulose and xylan. We have obtained the following results during last two years.
1. HAPC,a compoment of AnCP,was prepared by expressing malE-hapC fusion gene in E.coli and used to raise an antibody to HAPC.With recombinant HAPC and its antibody, we succedeed to prove that AnCP comprised three subunits including HAPC.
2. CCAAT sequence was proved not to be involved in the induction of the gene. SRE (starch responsive element) and its binding protein, SREB,were suggested to play a vital role in induction of Taka-amylaseA gene.
3. CMC inducible cellulase gene, eglA,was isolated from A.nidulans and sequenced. Preliminary analysis as to the promoter region was also conducted to find any elements for induction or reperssion of eglA.
4. Xylanase gene, cgxA,isolated from C.gracile was characterized for itsrepressive element and repressor-likefactor which recognized the 13bp repressive element. Furthermore, the A.oryzae xylanase gene, xynFI,was also determined for its inducible element. A 35bp element appeared to be involved in inductive expression of the gene.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Masashi Kato: "The Aspergillus nldu lans CCAAT-binding factor,AnCP/AnCF,is a heteromeric protein analogous to the HAP complex of Soccharomyces cerevisioe." Mol.Gen.Genet.in press (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masashi Kato: "An Aspereillus nldulans nuckear protein.AnCP,inrolved in enhoncement of Takoamylose Asone expression binds to the CCAAT.contoining taaG2,andS and at A promoters." Mol.Gen.Genet.254. 119-126 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masashi Kato: "Sequance-specific binding sites in the Taka-amylase AG2 promoter for the CreA raptossor mediating carbon catabolite repression." Biosci.Biotech.Biochem.60. 1776-1779 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Noriyuki Kitamoto: "Molecular cloning,purificotion and characterbation of two endo-1,4-gluconases from Aspergillus oryzae KBN616." Appl.Microbiol.Biotechnol.46. 538-544 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kato et al.: "The Aspergillus nidulans CCAAT-binding factor, AnCP/AnCF,is a heteromeric protein analogous to the HAP complex of Saccharomyces cerevisiae." Mol.Gen.Genet.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kato et al.: "An Aspergillus nidulans nuclear protein, AnCP,involved in enhancement of Taka-amylase A gene" Mol.Gen.Genet.254. 119-126 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kato, et al.: "Sequence-specific binding sites in the Taka-amylase A G2 promoter for the CreA repressor mediating carbon catabolite repression." Biosci.Biotech.Biochem.60. 1776-1779 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Kitamoto et al.: "Molecular cloning, purification and characterization of two endo-1,4-glucanases from Aspergillus oryzae KBN616." Appl.Microbiol.Biotechnol.46. 538-544 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masashi Kato: "The Aspergillus nidusans CCAAT-biding factor,AnCPl,AnCF,is a heteromeric protein analogous to the HAP complex of socchromyces cerevisiae" Mol.Gen.Genet.in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Msashi Kato: "An Aspergillus nidulans nuckar protein. AnCP,involved in enhancement of takaamylose Asone expression binds to the CCAAT.contoining taaG2,and S and gat A promoters" Mol.Gen.Genet.254. 119-126 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masahi Kato: "Seguence.specific binding sites in the Taka-amylase AG2 promoter for the CreA repressor mediating carbon catabolite repression" Biosci.Biotech.Biocchem.60. 1776-1779 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Noriyuki Kitamoto: "Molecular cloning,purificotion and characterzation of two endo-1,4-gluconases from Aspergillus oryzae KBN616" Appl.Microbiol.Biotechnol.46. 538-544 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masashi Kato: "An Aspergillus nidulans nuclear protein,AnCP,involved in entranciment of Taka-amylase A gene expression,bints to the CCAAT-containing TAAG^2,amdk and gal A promoters." Mol.Gen.Genet.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Noriyuki Kitamoto: "Molecular cloning purification and characterization of two ondo-1,4-glucanases from Aspergillus onyzae KBN 616" Appl.Microbiol.Biotechnol.46. 538-544 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masashi Kato: "Seguence specific binding sites in the Taka-amylase A G2 promoter for the Cre A represson mediating carbon catabolite nepression" Biosci.Biotechnol.Biochem.60. 1776-1779 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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