| Project/Area Number |
08456059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
IWAMURA Hajime Kyoto Univ., Appl.Life Sei., Prof., 農学研究科, 教授 (30026570)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Atsusi Kyoto Univ., Appl.Life Sei., Instr., 農学研究科, 助手 (80281103)
HIRAI Nobuhiro Kyoto Univ., Appl.Life Sei., Instr., 農学研究科, 助手 (00165151)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | wheat / maize / defense mechanism / benzoxazinones / glucosidase / antifungal substances |
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| Research Abstract |
Benzoxazinones are known as defense compounds in wheat, maize and rye, and occur as glucosides. Upon infection or insect attack, they are rapidly hydrolyzed to produce the aglycones. The aglycones exhibit wide range of fugi-and bacteriostatic activity as well as antifeeding activity against insects. In this study, we found that free benzoxazinones occur transiently during non-autotrophic stage of growth. This appearance and disappearance were little affected by infection and wounding, and thus thought to be a programd event.
Benzoxazinone glucoside beta-glucosidade activity was identified in extracts of maize and wheat seedlings, and purified to an apparent homogeneity by ion-exchange and gel chromatographies. Characterization made for this fraction revealed that the glucosidase has a very high specificity for benzoxazinone glucosides, indicating that a specific glucosidase occurs for the production of active benzoxazinones in the juvenile stage. Also identified in the extracts were a glucosyltransferase with a high specificity for benzoxazinones. The set of results suggested that benzoxazinones occur in a programd manner as defense at the vulnerable juvenile stage of qrowth, and specific glucosisade and glucosyltransferase are involved in this event.
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