| Project/Area Number |
08456128
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
農業機械学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
HAYAKAWA Isao KYUSHU UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (30038252)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | quick decompression / pressure sterilization / spore sterilization / Bacillus stearothermophilus / mild temperature / bacterial cell break / heat tolerance spore / strain rate / 耐体性胞子 / Bacillus stearothemophilus / 菌耐破壊 |
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| Research Abstract |
Adiabatic Expansion Sterilizer in a moment is developed by using combination works between a taper seal valve and link-motion mechanism, which is able to run over 380,000MPa/s at 200 MPa of decompression velocity. Mechanism of present high pressure sterilization on Bacillus stearotermophilus IFO 12550 spore was physical destruction of the spore coat by adiabatic expansion of water permeated through the spore coat under pressure. Water volume of permeated through the spore coat under pressure is rate-determining on spore disintegration, which was influenced by temperature. Sterilization was promoted over 75ﾟC.The spore of Bacillus stearothermophilus IFO 12550 was almost ruptured at 100 MPa. D value under 200 MPa compression is 17 min at 75ﾟC,11 min at 85ﾟC and 6 min at 95ﾟC respectively, when B.stearothermophilus spore IFO 12550 (D100ﾟC values=3000 min) are treated by 20 min. Spore coats are broken to brittle fracture exactly when balk strain velocity of water exceeds in 42.4/s (at decompression of '00 MPa).
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