| Project/Area Number |
08456136
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
SASAKI Yasuyuki TOHOKU UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (90005637)
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| Co-Investigator(Kenkyū-buntansha) |
HAGINO Akihiko TOHOKU UNIVERSITY,FACULTY OF AGRICULTURE,RESEARCH ASSOCIATE, 農学部, 助手 (80156249)
KATOH Kazuo TOHOKU UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (60091831)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | IGF-I / colostrum / apoptosis / tyrosine kinase / ペプチド |
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| Research Abstract |
We found a cell-growth inhibiting factor in partially purified fraction from bovine colostrum using an ion-exchange chromatography. The factor induced apoptosis in mouse LM fibroblasts. It is well known that bovine colostrum contains IGF-I,so we measured IGF-I concentrations of the fraction. The fraction contained about 500ng/mg protein of IGF-I.Then, effects of IGF-I on cell proliferation were determined using three cell lines, LM,L6, and Balb/c 3T3 cells. To our surprise, IGF-I induced cell death of LM cells, while it stimulated proliferation of L6 and Balb/c 3T3 cells in low FCS condition. Herbimycin A,a tyrosine kinase inhibitor, inhibited cell death induced by IGF-I in LM cells.
These data suggests that IGF-I induces cell death of LM cells and the action involves activation of tyrosine kinase.
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