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Neuroendocrine mechanism mediating the nutritional infertility

Research Project

Project/Area Number 08456151
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionNagoya University

Principal Investigator

MAEDA Kei-ichiro  Nagoya University School of Agricultural Sciences Associate Professor, 農学部, 助教授 (30181580)

Co-Investigator(Kenkyū-buntansha) MATSUSHIMA Toshiya  Nagoya University School of Agricultural Sciences Associate Professor, 農学部, 助教授 (40190459)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥4,900,000 (Direct Cost: ¥4,900,000)
Keywordsluteinizing hormone / blood glucose / estrogen / estrogen receptor / A2 region of solitary truct nucleus / paraventricular nucleus / noradrenergic nurons / ノルアドレナリン作動性神経 / 2-deoxyglucose / glucoprication / c-fos / 最後野 / 孤束核 / 室傍核
Research Abstract

Glucose availability would be one of the major factors involved in the suppressed reproductive functions under malnutrition in mammalian species. In the present study, the role of glucose availability in regulating gonadotropin-releasing hormone (GnRH)/Iuteinizing hormone (LH) release in female rats. The sensors for detecting the blood glucose level to regulate LH secretion and feeding behavior have been considered to be located in the hypothalamus and medulla oblongata in rats. The present study focussed on the localization of glucose sensors in the area postrema using Fos immunohistochemistry as a marker of neuronal activation.
Peripheral administration of a glucose antagonist, 2-deoxyglucose (2DG), induced a massive expression of Fos protein in the area postrema (AP), solitary tract nucleus (NTS) and paraventricular nucleus of the hypothalamus in ovariectomized and estradiol-primed ovariectomized rats. Dual immunohistochemistry with Fos and various cell markers, such as GFAP and MAP-2, showed that Fos-expressing cells in the AP would be neuronal but not glial cells. These cell were not catecholaminergic because any Fos-like immunoreactivities in the AP were not co-localized with tyrosine hydroxy lase immunoreactivity. A part of the Fos-expressing neruons in the NTS was catecholaminergic.
These results indicate that a population of non-catecholaminergic neurons in the AP could be involved in sensing low glucose availability to regulate reproductive functions as well as feeding behavior n female rats.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Nagatani, Shoji: "Reduction of glucose availability suppresses pulsatile LH release in female and male rats." Endocrinology. 137. 1166-1170 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murahashi, Kumiko: "Suppression of LH pulses by restriction of glucose availability is mediated by sensors in the brain stem." Endocrinology. 137. 1171-1176 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagatani, Shoji: "A rapid suppressive effect of estrogen in the paraventricular nucleus on pulsatile LH release in fasting-ovariectomized rats." Journal of Neuroendocrinology. 8. 267-273 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Estacio, M.A.C.: "Effect of fasting and immobilization stress on estrogen receptor immunoreactivity in the brain in ovariectomized female rats." Brain Research. 717. 55-61 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maeda, Kei-ichiro: "Novel estrogen feedback sites associated with stress-induced suppression of luteinizing hormone secretion in female rats." Celluler and Molecular Neurobiology. 16. 311-324 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 前多 敬一郎: "飢餓ストレスと生殖機能" 運動生化学. 9. 13-22 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawakami, Shin-ichi: "Glial and neuronal localization of neuronal nitric oxide synthase immunoreactivity in the median eminence of female rats." Brain Research. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawakami, Shin-ichi: "Evidence for terminal regulation of GnRH release by excitatory amino acids in the median eminence in female rats : A dual immunoelectronmicroscopical study." Endocrinology. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 市川 真澄他: "脳と生殖-GnRH神経系の進化と適応" 学会出版センター, 233 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagatani, Shoji: "Reduction of glucose availability suppresses pulsatile LH release in female and male rats." Endocrinology. 137. 1166-1170 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murahashi, Kumiko: "Suppression of LH pulses by restriction of glucose availability is mediated by sensors in the brain stem." Endocrinology. 137. 1171-1176 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagatani, Shoji: "A repid suppressive effect of estrogen in the paraventricular nucleus on pulsatile LH release in fasting-ovariectomized rats." Joural of Neuroendocrinology. 8. 267-273 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Estacio, M.A.C.: "Effect of fasting and immobilization stress of estrogen receptor immunoreactivity in the brain in ovariectomized female rats." Brain Research. 717. 55-6 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Maeda, Kei-ichiro: "Novel estrogen feedback sites associated with stress-induced suppression of luteinizing hormone secretion in female rats." Celluler and Molecular Neurobiology. 16. 311-324 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawakami, Shin-ichi: "Glial and neuronal localization of neuronal nitric oxide synthase immunoreactivity in the median eminence of female rats." Brain Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawakami, Shin-ichi: "Evidence for terminal regulation of GnRH release by excitatory amino acids in the median eminence in female rats : A dual immunoelectronmicroscopical study." Endocrinology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murahashi, Kumiko: "Increase in in vitro glutamate release in the mediobasal hypothalamus during progesterone-enhanced LH surge in estrogen-primed ovariectomized rats." Journal of Reproduction and Development. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagatani,Shoji: "Reduction of glucose availability suppresses pulsatile LH release in female and male rats" Endocrinology. 137. 1166-1170 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Murahashi,Kumiko: "Suppression of LH pulses by restriction of glucose availability is mediated by sensors in the brain stem" Endocrinology. 137. 1171-1176 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagatani,Shoji: "A rapid suppressive effect of estrogen in the paraventricular nucleus on pulsatile LH release in fasting-ovariectomized rats" Journal of Neuroendocrinology. 8. 267-273 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Estacio,M.A.C.: "Effect of fasting and immobilization stress on estrogen receptor immunoreactivity in the brain in ovariectomized female rats" Brain Research. 717. 55-61 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Maeda,Kei-ichiro: "Novel estrogen feedback sites associated with stress-induced suppression of luteinizing hormone secretion in female rats" Celluler and Molecular Neurobiology. 16. 311-324 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 前多敬一郎: "飢餓ストレスと生殖機能" 運動生化学. 9. 13-22 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kawakami,Shin-ichi: "Glial and neuronal localization of neuronal nitric oxide synthase immunoreactivity in the median eminence of female rats" Brain Research. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kawakami,Shin-ichi: "Evidence for terminalregulation of GnRH release by excitatory amino acids in the median eminence in female rats: A dual immunoelectronmicroscopical study" Endocrinology. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 市川真澄他: "能と生殖-GnRH神経系の進化と適応" 学会出版センター, 233 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagatani,Shoji: "Reduction of glucose availability suppresses pulsatile LH release in female and male rats." Endocrinology. 137. 1166-1170 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Murahashi,Kumiko: "Suppression of LH pulese by restriction of glucose availability is mediated by sensors in the brain stem." Endocrinology. 137. 1171-1176 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagatani,Shoji: "A rapid suppressive effect of estrogen in the paraventricular nucleus on pulsatile LH release in fasting-ovariectomized rats." Journal of Neuroendocrinology. 8. 267-273 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Estacio,M.A.C.: "Effect of fasting and immobilization stress on estrogen receptor immunoreactivity in the brain in ovariectomized female rats." Brain Research. 717. 55-61 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 前多敬一郎: "生殖機能を制御する脳内メカニズム:GnRH Pulse Generator." 獣医畜産新報. 49. 33-38 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagatani,Shoji: "Paraventricular norepinephrine release mediates glucoprivic suppresion of pulsatile LH secretion." Endocrinology. 137. 3183-3186 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Maeda,Kei-ichiro: "Novel estrogen feedback sites associated with stress-induced suppression of luteinizing hormone secretion in female rats." Celler and Molecular Neurobiology. 16. 311-324 (1966)

    • Related Report
      1996 Annual Research Report
  • [Publications] Estacio,M.A.C.: "Vagus nerve mediates the increase in estrogen receptors the hypothalamic paraventricular nucleus and nucleus of the slitary tract during fasting in ovariectomized rats." Neuroscience Letters. 208. 25-28 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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