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MORECULAR GENETICAL INVESTIGATION OF DOMESTIC ANIMALS AND POULTRY USING HIGHLY POLYMORPHIC MICROSATELLITE DNA

Research Project

Project/Area Number 08456152
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

KUNIEDA Tetsuo  OKAYAMA UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (80178011)

Co-Investigator(Kenkyū-buntansha) MORITOMO Yasuo  KYUSYU TOKAI UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (70148972)
OIKAWA Takuro  OKAYAMA UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (90160407)
SATO Katsunori  OKAYAMA UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (10033325)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1996: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsMICROSATELLITE / DOMESTIC ANIMAL / POULTRY / QUAIL / CATTLE / CHONDRODYSPLASIA / MUTATION / MAPPING / 遺伝病 / マイクロサテライトDNA / 反復DNA配列 / 連鎖解析 / 褐毛和種 / FGFR3 / 高多型反復配列 / 近交係数 / 染色体
Research Abstract

The aims of the present study are to construct genetic linkage maps of the Japanese quail, to identify gene responsible for chondrodysplasia of Japanese brown cattle, and to characterize genetic feature of the particular population of Japanese brown cattle.
To isolate microsatellite DNA of Japanese quail, microsatellite enriched genomic DNA library was screened with dinucliotide repeats. Consequently, we isolate clones containing CA dinucleotide repeats, but the dinucleotide repeats of most of these clone are interrupted by another sequences. These interrupted dinucleotide repeats are not likely to be polymorphic and thus could not be used as marker loci.
In a particular population of Japanese brown cattle, a chondrodysplastic dwarfism is inherited as an autosomal recessive trait. By using 2200 bovine microsatellite markers and a paternal half-sib pedigree including 48 affected and 28 normal animals, we investigated linkage between the locus responsible for the bovine chondrodysplasi and microsatellite loci. Consequently, we mapped the locus on a particular region of bovine chromosome 6. The region of the bovine chromosome 6 is homologous to regions of human and mouse choromosomes on which FGFR3 gene is localized. Since this gene is known to be responsible for human chondrodysplasia, the FGFR3 gene is a candidate gene for the bovine chondrodysplasia.
Genome wide scanning of the 5 cattle obtained from a particular population of Japanese brown cattle were performed by using microsatellite markers. About 50% of the microsatellite markers, which are polymorphic in the population, were homozygous condition in the cattle. The significant portion of these homozygous loci were revealed to localize on particular region of chromosomes. These findings suggest that the microsatellite loci could be a useful markers for investigating the genetic feature of populations of domestic animals.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Kunieda, T.: "A mutation in endothelin B receptor gene …" DNA Res.3. 101-105 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Karaki, H.: "Lack of endothelin ET_B receptor binding …" Biochem.Biophys.Res.Commun. 222. 139-143 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Valdivia, R.P.A.: "Tayping of Tabby locus in mouse …" Exp.Anim.45. 395-398 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kunieda, T.: "Localization of Moloney murine leukemia …" Mamm.Genome. 7. 924-925 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Simonaro, C.M.: "Bone marrow transplantation in newborn …" Transplantation. 63. 1386-1393 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuji, T.: "Cloning and mapping of the mouse Gpx2 …" J.Vet.Med.Sci. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kunieda, T.: "The gene encoding anti-mulerian …" Mamm.Genome. 9. 259-259 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kumagai, T.: "Nucleotide sequence of endothelin B …" Exp.Anim. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oikawa, T.: "Study of animal model prediction for a …" Anim.Technol.67. 755-763 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuzuki, M.: "Fawn-2 : A dominant plumage color mutation …" J.Hered. 87. 248-252 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oikawa, T.: "Comparison of breeding system between …" Asian-Australasian.J.Anim.Sci.10. 428-434 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oikawa, T.: "Treating small herds as fixed or random …" J.Anim.Breeding Genetics. 114. 177-183 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yanai, T.: "Spontaneous Vascular mineralization …" J.Vet.Med.Sci.58. 35-40 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kunieda, T., Kumagai, T., Tsuji, T., Ozaki, T., Karaki, H., and Ikadai, H.: "A mutation in endothelin-B receptor gene causes myenteric aganglionosis and coat color spotting in rat." DNA Res.3. 101-105 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Karaki, H., Mitui-Saito, M., Takimoto M., Oda, K., Okada, T., Ozaki, T., and Kunieda, T.: "Lack of endothelin ETB receptor binding and function in the rat with a mutant ETB receptor gene." Biochem.Biophys.Res.Commun.222. 139-143 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Valdivia, R.P.A., Kunieda, T., Hattori, M., and Yoyoda, Y.: "Typing of Tabby locus in mouse preimplantation embryos by detection of a microsatellite marker." Exp.Anim.45. 395-398 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kunieda, T., Kobayashi, E., Tachibana, M., Ikadai, H.: "Localization of a Moloney murine leukemia virus integration site gene, Mlvi2, on rat chromosome 2." Mamm.Genom.7. 924-925 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Simonaro, C.M., Haskins, M.E., Kunieda, T., Evans, S.M., Visser, J.W., Schuchman, E.H.: "Bone marrow transplantation in newborn rats with mucopolysaccharidosis type VI : biochemical, pathological, and clinical findings." Transplantation. 63. 1386-1393 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuji, T., Watanabe, Y., Katoh, H., Sato, K., Kunieda, T.: "Cloning and mapping of the mouse Gpx2 gene encoding gastrointestinal glutathione peroxidase." J.Vet Med, Sci.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kunieda, T., Ojika, I., and Katoh, H.: "The gene encoding anti-Mulerian hormone type 2 receptor maps to mouse chromosome 1." Mamm.Genome. 9. 259 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kumagai, T., Wada, A., Tsudzuki, M., Nishimura, M., and Kunieda, T.: "Nucleotide sequence of endothelin-B receptor gene revealed the origin of the piebald mutation in the laboratory mouse." Exp.Anim.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Simonaro et al: "Bone marrow transplantation in newborn" Transplantation. 63. 1386-1393 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsuji et al: "Cloning and mapping of the mouse Gpx 2 gene" J.Vet.Med.Sci.60(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kunieda et al: "The gene encoding anti-Mulerian hormone" Mamm.Genome. 9(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 国枝哲夫: "家畜遺伝性疾患の原因遺伝子解明へ向けた" 獣医臨床遺伝研究会誌. 5. 3-8 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Kunieda et al: "A muctation in endothelin-B receptor geve causes ----" DNA Research. 3. 101-105 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Karaki et al: "Lack of endothelin ET_B receptor binding and ----" Biochem,Biophys.222. 139-143 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] R.Valdivia et al: "Typing of Xchromosome bearing Tabby allele in ----" Eyp.Amim. 45. 395-398 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Kunieda et al: "Localization of a Moloney murine Leukemia virus ----" Mamm.Genome. 7. 924-925 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 国枝哲夫: "牛における遺伝性疾患の分子遺伝学的解析" 獣医畜産新報J.V.M.49. 413-416 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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