| Project/Area Number |
08456158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ONODERA Takashi Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Professor, 大学院・農学生命科学研究科, 教授 (90012781)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Yasunobu Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Assistant Professor, 大学院・農学生命科学研究科, 助教授 (90251420)
MATSUMOTO Yoshitugu Univ.of Tokyo, Grad.Sch.of Agri.and Life Sci., Associate Professor, 大学院・農学生命科学研究科, 助教授 (00173922)
百渓 英一 農林水産省, 家畜衛生試験場, 室長
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | prion / mouse / gene targeting / gene / apoptosis / promoter / adenovirus / PCR |
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| Research Abstract |
Prions cause degenerative disease of the central nervous system in both humans and animals and are composed of the scrapie isoform of the prion protein (PrP^<SC>) (Prusiner, 1991 ; Chesebro et al., 1985 ; Oesch et al., 1985 ; Kretzschmar et al., 1986). The chromosomal PrP gene encodes both PrP^C and cellular isoform, PrP^C. The entire open reading frame (ORF) is contained within a single exon, which was considered to be separated from one or two exons encoding the 5' untranslated region of PrP mRNAs by a large intron. The cell surface localization of PrP^C together with its highly expression in the brains of neonatal hamsters suggested that PrP^C might function in cell recognition (Stahl et al., 1987 ; Mobley et al., 1998). Of particular interest are 5' upstream of a untranslated regions of so-called exon 1, which will work as a promoter for the transcription of PrP mRNA.These upstream region will help us to uncover the unknown promoter/enhancer system for PrP gene.
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