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Juvenile hormone receptor and molecular mechanism of gene expresion

Research Project

Project/Area Number 08456171
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionMie University

Principal Investigator

CHINZEI Yasuo  Mie University Faculty of Medicine, Professor, 医学部, 教授 (60024709)

Co-Investigator(Kenkyū-buntansha) YUDA Masao  Mie University Faculty of Medicine, Assistant, 医学部, 助手 (90293779)
ANDO Katsuhiko  Mie University Faculty of Medicine, Associate Professor, 医学部, 助教授 (90024710)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1996: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsJuvenile hormone / cyanoprotein / gene expression / transferrin / differential display / fumin / Vitellogenin / シアノプロテイン / 貯蔵蛋白質 / 転写調節 / 転写調節因子 / cDNAクローニング / ゲルシフトアッセイ
Research Abstract

To study the molecular mechanism of gene expression regulation by insect juvenile hormone (JH), we have analyzed JH responsive genes by mRNA Differential Display in the bean bug, Riptortus clavatus. JH analog, methoprene, was applied on the diapause female adults and extracted RNA from the fat body. We found many cDNA bands which showed differnence between JHA and solvent acetone treated animals in mRNA Differential Display. We picked up the cDNA which were induced or repressed cDNA by JH treatment. Using these cDNA we performed Northern Blot analyzes for JH or acetone treated fat body RNA,and identified two JH inducible genes (JI-1and JI-2) and three JH repressible genes (JR-! , JR-2 and JR-3). The analysis of the partial sequeces of these cDNA and data base homology search indicated that JI-1 and JI-2 had high homology to cyanoprotein-a and vitellogenin, respectively, which we have showed hemolymph storage hexamer and egg yolk protein precursor. JR-1 was showed high homology to transferrin in vertebrate and insects. On the other hand we could not find any genes which have homology to JR-2 and JR-3 and concluded these gene are novel. We cloned and sequenced full length of cDNA of JI-2 and JR-1 and analyzed the changes of mRNA level after JH tretment by NOrthern Blot analyzes. We also cloned genomic DNA and analyzed the upstream regulatory gene structure of JI-1 (CP-a), JI-2 (Vg) and JR-1 (Transferrin). Now three JH inducible and three repressible genes are available to compare the regulation of expression by JH in the same insect under the same hormonal condition.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Makoto Hirai et al.: "Identification and cDNA cloning of novel juvenile hormone responsive genes from fat body of the bean bug, Riptortus clavatus by mRNA differential display." Insect Biochem Mol.Biol.28(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] P.Waidhet-Kouadio et al.: "Purification and characterization of a thrombin inhibitor from the salivary glands of a malarian vector mosquito,Anopheles stephensi." Biochemi Biophy.Acta.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jianxin Sun et al.: "Characterization and cDNA cloning of a hemoprotein in the salivary glands of a blood sucking insect,Rhodnius prolixus." Insect Biochem.Mol.Biol.28(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masao Yuda et al.: "Expression,reconstitution and characterization of prolixin-S as a vasodilator: a salivary gland nitric-oxide-binding hemoprotein of Rhodnius prolixus." Eur.J.Biochem.249. 337-342 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsro S. et al.: "Vitellogenins and vitellins in the bean bug,Riptortus clavatus" Arch.Insect Biochem.Physiol.31. 395-412 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miura K.et al.: "Cloning of mRNA sequences for two antibacterial peptides in a hemipteran insect,Riptortus clavatus." Zool.Sci.13. 111-117 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Miura K.et al.: "Two juvenile hormone-enhancable and-suppressible haxamerin subunits from a hemipteran insects,Riptorfus clavatus cloning developmental regulation and phylogenetic analysis." Gene. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuoka et al.: "Depletion of salivary gland proteins in Anopheles stephenst.(Diptera:Cultcidae)on blood feeding,and induction of antibodies to the proteins in mice being fed." Med.Entomol.and Zool.48. 211-218 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yuda M.et al.: "cDNA cloning,expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect,Rhodnius prolixus." Eur.J.Biochem. 242. 807-812 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jianxin Sun et al.: "Purification,characterization and cDNA cloning of anovel anti-coagulant heme protein(Prolixin-s)from the salivary glands of the blood sucking bug,Rhodnius prolixus." Thromb.Haemost.75. 573-577 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yuda M.et al.: "Identification and activities of anticoagulants in the salivary glands of the blood sucking kissing bug,Rhodnius prolixus." Med.Entomol.Zool. 47. 263-272 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makoto Hirai, Masao Yuda, Tetsuro Shinoda and Yasuo Chinzei: "Identification and cDNA cloning of novel juvenile hormone responsive genes from fat body of the bean bug, Riptortus clavatus by mRNA differential display" Insect Biochem Mol.Bioll.28(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Philomene Waidhet-Kouadio, Masao Yuda, Katsuhiko Ando and Yasuo Chinzei: "Purification and characterization of a thrombin inhibitor from the salivary glands of a malarian vector mosquito, Anopheles stephensi." Biochemi Biophy.Acta.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jianxin Sun, Masao Yuda, Ken Miura and Yasuo Chinzei: "Characterization and cDNA cloning of a hemoprotein in the salivary glands of a blood sucking instect, Rhodnius prolixus." Insect Biochem.Mol.Biol.28(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ken Miura, Tetsuro Shinoda, Mamoru Yura, Sen Nomura, Katsumi Kamiya, Masao Yura and Yasuo Chinzei: "Two juvenile hormone-enhancable and suppressible hexamerin subunits from a hemipteran insects, Riptortus clavatus : cloning, developmental regulation and phylogenetic analysis." Gene. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masao Yoda, Kuniko Higuchi, Jianxin Sun, Yasuko Kureishi, Masaaki IIto and Yasuo Chinzei: "Expression, reconstitution and characterization of prolixin-S as a vasodilator : a salivary gland nitric-oxide-binding hemoprotein of Rhodnius prolixus." Eur.J.Biochem.249. 337-342 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiroyuki Matsuoka, Hisashi Matsubara, Philomene Waidhet, Katsuhiko Ando and Yasuo Chinzei: "Depletion of salivary gland proteins in Anopheles stephenis (Diptera : Culicidae) on blood feeding, and induction of antibodies to the proteins in mice being fed." Med.Entomol.and Zool.48. 211-218 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masao Yuda, Makoto Hirai, Ken Miura, Haruo Matsumura, Katsuhiko Ando and Yasuo Chinzei: "cDNA cloning, expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect, Rhodnius prolixus." Eur.J.Biochem.242. 807-812 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tetsro Shinoda, Ken Miura, and Yasuo Chinzei: "Vitellogenins and vitellins in the bean bug, Riptortus clavatus (Heteroptera : Alydidae) : Purification, immunological identification and induction by juvenile hormones." Arch.Insect Biochem.Physiol.31. 395-412 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jianxin Sun, Masahiro Yamaguchi, Masao Yuda, Ken Miura, Hiroyuki Takeya, Makoto Hirai, Hiroyuki Masuoka, Katsuhiko Ando, Takashi Watanabe, Koji Suzuki and Yasuo Chinzei: "Purification, characterization and cDNA cloning of a novel anti-coagulant heme protein (Porlixin-S) from the salibavy glands of the blood sucking bug, Rhodnius prolixus." Thromb.Haemost.75. 573-577 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ken Miura, Satoru Ueno, Katsumi Kamiya, Jun Kobayashi, Hiroyuki Matsuoka, Katsuhiko Ando and Yasuo Chinzei: "Cloning of mRNA sequences for two antibacterial peptides in a hemipteran insect, Riptortus clavatus." Zool.Sci.13. 111-117 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masao Yuda, Jianxin Sun, Masahiro Yamaguchi, Katsuhiko Ando and Yasuo Chinzei: "Identification and activities of anticoagulants in the salivary glands of the blood sucking kissing bug, Rhodnius prolixus." Med.Entomol.Zool.47. 263-272 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makoto Hirai et al.: "Identification and cDNA cloning of novel juvenile hormone responsive genes from fat body of the bean bug,Riptortus clavatus by mRNA differential display." Insect Biochem Mol.Biol.28(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] P.Waidhet-Kouadio et al.: "Purification and characterization of a thrombin inhibitor from the salivary glands of a malarian vector mosquito,Anopheles stephensi." Biochemi Biophy.Acta.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Jianxin Sun et al.: "Characterization and cDNA cloning of a hemoprotein in the salivary glands of a blood sucking insect,Rhodnius prolixus." Insect Biochem.Mol.Biol.28(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masao Yuda et al.: "Expression,reconstitution and characterization of prolixin-S as a vasodilator:a salivary gland nitric-oxide-binding hemoprotein of Rhodnius prolixus." Eur.J.Biochem.249. 337-342 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tetsro S.et al.: "Vitellogenins and vitellins in the bean bug,Riptortus clavatus" Arch.Insect Biochem.Physiol.,. 31. 395-412 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Miura K.et al.: "Cloning of mRNA sequences for two antibacterial peptides in a hemipteran insect,Riptortus clavatus." Zool.Sci.,. 13. 111-117 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masao Yuda et al: "Identification and activities of anticoagulant in the salivary glands of the blood sucking bug,Rhodnius prolixus." Med.Entomol.Zool.,. 47. 263-272 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Jianxin Sun,et.al: "Purification,characterization and cDNA cloning of a novel anticoagulant of the intrinsic pathway,(prolixin-S),from salivary glands of the blood sucking bug,Rhodnius prolixus." Thrombosis and Haemostasis. 75. 573-577 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masao Yuda, et al.: "cDNA cloning,expression and characterization of nitric-oxide synthase from the salivary glands of the blood-sucking insect Rhodnius prolixus." Eur.J.Biochem.242. 807-812 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiroyuki M.et al.,: "Induction of anti-malarial transmission blocking immunity with a recombinant ookinete surface antigen of Pasmodium berghei produced in silkworm larvae" Vaccine. 14. 120-126 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tetsro S.et al.: "Vitellogenins and vitellins in the bean bug,Riptortus clavatus" Arch.Insect Biochem.Physiol.,. 31. 395-412 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yukiyoshi S.et al.: "Effect of temperature to Plasmodium berghei and P.yoelii on mosquito stage in Anopheles stephensi." Jpn.J.Parasitol.,. 45. 98-104 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Miura K.et al.: "Cloning of mRNA sequences for two antibacterial peptides in a hemipteran insect,Riptortus clavatus." Zool.Sci.,. 13. 111-117 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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