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Roles of proteases on cell-to-cell adhesion and deadhesion

Research Project

Project/Area Number 08457013
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

SHIOSAKA Sadao  NAIST,STRUCT.CELL BIOL., PROFESSOR, バイオサイエンス研究科, 教授 (90127233)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Nobuaki  OSAKA MEDICAL CENTER FOR ATERNAL AND CHILD HEALTH,DIRECTOR, 部長 (10250341)
YOSHIDA Shigetaka  NAIST,STRUCT.CELL BIOL., ASS.PROFESSOR, バイオサイエンス研究科, 助教授 (20230740)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsNEUROPSIN / MIGRATION / CELL-TO-CELL ADHESION / CELL-TO-CELL DEADHEASION / FIBRONECTIN / SERINE PROTEASE / NEURON / KERATINOCYTE
Research Abstract

Some proteases have been suggested to be related to cell dynamics in such processes as cell death, cell migration, cell-to-cell adhesion and deadhesion, and neural process elongation, pathfinding and axonal rearrangement, we postulated that (a) novel protease (s) might be released from neurons and relate to various cell dynamics described above.
Neuropsin was cloned from the mouse brain and was shown to be localized in mouse hippocampal pyramidal neurons and also epidermal keratinocytes. Recombinant neuropsin effectively cleaved fibronectin which is a major ECM protein expressed in variety of tissues. The specific cleavage by recombinant neuropsin as shown in the present study might directly affect fibronectin's functions as a cell adhesion molecule, because the N-terminal 5 FnI domains contain the main fibrin-binding site. Cell migration and cell atachment activity were analyzed with model system using CHO cell which expressing alpha5beta1 integlin. Recombinant neuropsin effectively decreased these activity when fibronectin was used as cell adhesion molecule.
Neuropsin knock-out mouse was made and histological, bichemical, physiological and behavioral analysis is now in progress.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Shimizu, T.et al.: "Characterization of recombinantand brain neuropsin" J.Biol.Chem.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Inoue, N.et al.: "Expression of neuropsin in the keratinizing epithelial tissue" J.Invest.Derm.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chen, Zu-Lin.et al.: "Expression of neuropsin mRNA in the mouse embryo and pregnant uterus" J.Histochem.Cytochem.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Momota, Y.et al.: "Blockade of neuropsin,a serine protease,ameliorates kindling epilepsy" Eur.J.Neurosci.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akita, H.et al.: "Effect of oxidative stress on the expression of limbic-specific protease" Brain Res.769. 86-96 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chen, Zu-Lin.et al.: "Expression and activity-dependent changes of a novel limbic-serine protease" J.Neurosci.15. 5088-5097 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Inoue, N., Kuwae, K., Ishida-Yamamoto, A., Iizuka, H., Shibata, M., Yoshida, S., Kato, K.and Shiosaka, S.: "Expression of neuropsin in the keratinizing epithelial tissue-immunohistochemical analysis of wild-type and nude mice." J.Investigative Dermatology. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Momota, Y., Yoshida, M., Ito, J., Shibata, M., Kato, K., Sakurai, K., Matsumoto, K., and Shiosaka, S.: "Blockade of neuropsin, a serine protease, ameliorates kindling epilepsy." Eur.J.Neurosci.10. 760-764 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimizu, C., Yoshida, S., Shibata, M., Kato, K., Momota, Y., Matsumoto, K., Shiosaka, T., Midorikawa, R., Kamachi, T., Kawabe, A.and Shiosaka, S.: "Characterization of recombinant and brain neuropsin, a plasticity-related serine protease." J.Biol.Chem.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chen, Z-L., Momota, Y., Kato, K., Taniguchi, M., Inoue, N., Shiosaka, S., and Yoshida, S.: "Expression of neuropsin mRNA in the mouse embryo and the pregnant uterus." J.Histochem.Cytochem. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akita, H., Matsuyama, T., Iso, H., Sugita, M., and Yoshida, S.: "Effects of oxidative stress on the expression of limbic-specific protease neuropsin and avoidance learning in mice." Brain Res.769. 86-96 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okabe, A., Momota, S., Yoshida, S., Hirata, A., Ito, J., Nishino, S., Shiosaka, S.: "Kindling induces neuropsin mRNA in the mouse brain" Brain Res.729. 116-120 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki, J., Yoshida, S., Chen, Z-L., Momota, Y., Kato, K., Hirata, A.and Sadao Shiosaka: "Ontogeny of neuropsin mRNA expression in the mouse brain." Neurosci.Res.23. 345-351 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chen, Z-L., Yoshida, S., Kato, K., Momota, Y., Suzuki, J., Tanaka, T., Ito, J., Nishino, H., Aimoto, S., Kiyama, H.and Shiosaka, S.: "Expression and activity-dependent changes of a novel limbic-serine protease gene in the hippocampus." J.Neurosci.15. 5088-5097 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimizu,T.et al.: "Characterization of recombinant and brain neuropsin" J.Biol.Chem.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Inoue,N.et al.: "Expression of neuropsin in the keratinizing epithelial tissue" J.Invest.Derm.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Chen,Zu-Lin.et al.: "Expression of neuropsin mRNA in the mouse embryo and pregnant uterus" J.Histochem.Cytochem.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Momota,Y.et al.: "Blockade of neuropsin,a serine protease,ameliorates kindling epilepsy" Eur.J.Neurosci.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Akita,H.et al.: "Effect of oxidative stress on the expression of limbic-specific protease" Brain Res.769. 86-96 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Chen,Zu-Lin.et al.: "Expression and activity-dependent changes of a novel limbic-serine protease" J.Neurosci.15. 5088-5097 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Chen,Z-L.,: "Expression and activity-dependent changes of a novel limbic-serine protease" J.Neurosci.15. 5088-5097 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okabe,A.,: "Kindling induses neuropsin mRNA in the mouse brain" Brain Research. 728. 116-120 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki,J.,: "Ontogeny of Neuropsin mRNA Expression in the Mouse Brain" Neurosci.Res. 23. 345-351 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉田成孝: "海馬の機能蛋白質-可塑性関連因子" ブレインサイエンス. 7(2). 131-139 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 百田芳春: "ニューロン特異プロテアーゼと発達" Molecular Medicine. 33(6). 684-691 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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