| Project/Area Number |
08457023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Hokkaido University |
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Principal Investigator |
SAITO Hideya Hokkaido University School of Medicine Professor, 医学部, 教授 (20000929)
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| Co-Investigator(Kenkyū-buntansha) |
TOGASHI Hiroko Hokkaido University School of Medicine Lecturer, 医学部, 講師 (20113590)
YOSHIOKA Mitsuhiro Hokkaido University School of Medicine Associate Professor, 医学部, 助教授 (40182729)
MATSUMOTO Machiko Hokkaido University School of Medicine Instructor, 医学部, 助手 (70229574)
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| Project Period (FY) |
1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1996: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | serotonin / dopamine / anxiety / conditioned fear stress / antisense oligonucleotide |
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| Research Abstract |
We investigated the possible mechanism of the conditioned fear stress-induced freezing behavior. In addition, endogenous anxiogenic substances were searched using molecular pharmacological technique. Conditioned fear stress-induced dopamine and serotonin release in the prefrontal cortex but not freezing behavior were markedly inhibited by 7 days i.c.v. treatment with 5-HT_6 receptor mRNA antisense oligonucleotides. These results suggest that 5-HT_6 receptors might be involved in certain anxietic behavior. Furthermore, 5-HT_6 receptor antagonist may have properties for a new type of antianxiety. However, further experiments need to confirm the possibility that serotonin is a candidate of endogenous anxiogenic substance.
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