| Project/Area Number |
08457026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Niigata University |
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Principal Investigator |
YOSHIDA Yutaka (1997) Niigata University, School of Medicine Lecturer, 医学部, 講師 (40182795)
今井 昭一 (1996) 新潟大学, 医学部, 教授 (60013869)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Takaharu Niigata University, School of Medicine Assistant, 医学部, 助手 (60184561)
NAKAZAWA Mikio Niigata University, School of Medicine Associate professor, 医学部, 助教授 (80143759)
吉田 豊 新潟大学, 医学部, 講師 (40182795)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥6,900,000 (Direct Cost: ¥6,900,000)
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| Keywords | IP_3 receptor / cGMP-dependent protein kinase / cyclic GMP / vascular smooth muscle / plasma membrane calcium pump / cyclic GMP / cyclic GMP依存性タンパク質リン酸化酵素 |
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| Research Abstract |
To furhter clarify the role of phosphorylation of IP_3 receptor by protein kinase G (PKG) in the PKG induced stimulation of plasma membrane calcium pump (PMCA) of the vasucular smooth muscle, the effect of removal of IP_3 receptor on the stimulation was examed in the partially purified preparation of PMCA from porcine aorta, in which a definite stimulation of PMCA actrivity was observed upon activation of PKG.Contrary to expectation, removal of IP_3 receptor by specific immunoprecipitation with an antibody specific to type 1 IP_3 receptor did not affect the PKG stimulation of PMCA activity : the stimulation of PMCA was still observed even in the absence of IP_3 receptor. Furthermore, PKG stimulated PMCA in a highly purified preparation completely devoid of IP_3 receptor, only when the concentrations of PMCA were low (10 to 20nM), at which the enzyme is known to be in a monomeric form, while it failed to stimulate when the concentration of the enzyme was increased to 40 nM at which the enzyme is known to take an oligomeric form, and become fully actived and insensitive to calmodulin. Heat inactivated PKG,and PKG without cGMP did not produce stimulation. In addition, type Ialpha but not type Ibeta PKG was found to stimulate PMCA.It was concluded that PKG stimulates the PMCA in a monomeric form without phosphorylation of the ATPase, and that, of two PKG isozymes found in the vascular smooth muscle, only type Ialpha PKG participates in the stimulation.
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