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Pysiological siginificance of 240K protein of vascular smooth muscle

Research Project

Project/Area Number 08457026
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionNiigata University

Principal Investigator

YOSHIDA Yutaka (1997)  Niigata University, School of Medicine Lecturer, 医学部, 講師 (40182795)

今井 昭一 (1996)  新潟大学, 医学部, 教授 (60013869)

Co-Investigator(Kenkyū-buntansha) ISHIBASHI Takaharu  Niigata University, School of Medicine Assistant, 医学部, 助手 (60184561)
NAKAZAWA Mikio  Niigata University, School of Medicine Associate professor, 医学部, 助教授 (80143759)
吉田 豊  新潟大学, 医学部, 講師 (40182795)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsIP_3 receptor / cGMP-dependent protein kinase / cyclic GMP / vascular smooth muscle / plasma membrane calcium pump / cyclic GMP / cyclic GMP依存性タンパク質リン酸化酵素
Research Abstract

To furhter clarify the role of phosphorylation of IP_3 receptor by protein kinase G (PKG) in the PKG induced stimulation of plasma membrane calcium pump (PMCA) of the vasucular smooth muscle, the effect of removal of IP_3 receptor on the stimulation was examed in the partially purified preparation of PMCA from porcine aorta, in which a definite stimulation of PMCA actrivity was observed upon activation of PKG.Contrary to expectation, removal of IP_3 receptor by specific immunoprecipitation with an antibody specific to type 1 IP_3 receptor did not affect the PKG stimulation of PMCA activity : the stimulation of PMCA was still observed even in the absence of IP_3 receptor. Furthermore, PKG stimulated PMCA in a highly purified preparation completely devoid of IP_3 receptor, only when the concentrations of PMCA were low (10 to 20nM), at which the enzyme is known to be in a monomeric form, while it failed to stimulate when the concentration of the enzyme was increased to 40 nM at which the enzyme is known to take an oligomeric form, and become fully actived and insensitive to calmodulin. Heat inactivated PKG,and PKG without cGMP did not produce stimulation. In addition, type Ialpha but not type Ibeta PKG was found to stimulate PMCA.It was concluded that PKG stimulates the PMCA in a monomeric form without phosphorylation of the ATPase, and that, of two PKG isozymes found in the vascular smooth muscle, only type Ialpha PKG participates in the stimulation.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] M.O.Islam: "Isolation and characterization of vascular smooth muscle inositol 1,4,5-trisphosphate receptor" Biochm.J. 316. 295-302 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Hirono: "Expression of inducible nitric oxide synthase in rat experimental autoimmune myocarditis with special reference to changes in cardiac hemodynamics" Circ.Res.80. 11-20 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yoshida: "Structure and function of inositol 1,4,5-trisphosphate receptor" Jpn.J.Pharmacol.74. 125-137 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Kawada: "cGMP-kinase mediates cGMP-and cAMP-induced Ca^<2+> desensitization of skinned rat artery" Eur.J.Pharmacol.323. 75-82 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yoshida: "Stimulation of plasma membrane Ca^<2+>-pump ATP ase of vascular smooth muscle by cGMP-dependent protein kinase: Functional reconstitution with purified proteins" Mol.Cell.Biochem.in press. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 今井 昭一: "NO関連血管拡張薬の作用機序" 日本歯科麻酔学会雑誌. 25. 671-682 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.O.Islam: "Isolation and characterization of vascular smooth muscle inositol 1,4,5-trisphosphate" Biochem.J.316. 295-302 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Hirono: "Expression of inducible nitric oxide synthase in rat experimental autoimmune myocarditis with special reference to changes in cardiac hemodynamics" Circ.Res.80. 11-20 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yoshida: "Structure and function of inositol 1,4,5-trisphosphoate receptor" Jpn.J.Pharmacol.74. 125-137 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Kawada: "cGMP-kinase mediates cGMP-and cAMP-induced Ca^<2+> desensitization of skinned rat artery" Eur.J.Pharmacol.323. 75-82 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Yoshida: "Stimulation of plasma membrane Ca^<2+>-pump ATPase of vascular smooth muscle by cGMP-dependent protein kinase : Functional reconstitution with purified proteins" Mol.Cell.Biochem.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M. O. Islam: "Isolation and characterization of vasular smooth muscle inositol 1, 4, 5 - trisphosphate receptor." Biochem. J.316. 295-302 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] S. Hirono: "Expression of inducible nitric oxide synthase in rat experimental autoimmune myocarditis with special refernce to changes in cardiac hemodynamics." Circ. Res.80. 11-20 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y. Yoshida: "Structure and function of inositol 1, 4, 5 - trishosphate receptar." Jpn. J. Pharmacol.74. 125-137 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T. Kawada: "cGMP - kinase mediates cGMP and cAMP - induced Ca^<2+> desensitization of skinned rat artery." Eur. J. Pharmacol.323. 75-82 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y. Yoshida: "Stimulation of plasma membrane Ca^<2+> - pump ATPase of vascular smooth muscle by cGMP - dependent protein kinase : Functional reconstitution with purified proteins." Mol. Cell. Biochem. in Press. (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 今井昭一: "NO関連血管拡張薬の作用機序" 日本歯科麻酔学会雑誌. 25. 671-682 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Yoshida: "Stimulation of plasma membrane ca_<2+>-pump ATPase of vascular smooth muscle by cGMP-dependent protein kinase : Functional reconstitution with purified proteins" Mal. Cell. Biochem. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Yoshida: "Structure and function of inosital 1,4,5-trisphosphate receptor" Jpn. J. Pharmacol. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Kawada: "cGMP-kinase mediates cGMP-and cAMP-induced Ca^<2+>desensitization of skinned rat artery" Eur. J. Pharmacol. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] S. Hirono: "Expression of inducible nitric oxide synthase in rat experimental autoimmune myocarditis with special reference to changes in cardiac hemodynamics" Circ. Res. 80. 11-20 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M. O. Islam: "Isolation and characterization of vascular smoth muscle inositol 1,4,5-Trisphosphate receptor" Biochem. J. 316. 295-302 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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