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Cloning and analysis of the genes during the induction of mouse embryongenesis

Research Project

Project/Area Number 08457037
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionOsaka University

Principal Investigator

NAKANO Toru  BIKEN,DMCB,Professor, 微生物病研究所, 教授 (00172370)

Co-Investigator(Kenkyū-buntansha) ERA Takumi  BIKEN,DMCB,Research associate, 微生物病研究所, 助手 (00273706)
高橋 知巳  大阪大学, 微生物病研究所, 助手 (70283801)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1996: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordscDNA cloning / hematopoiesis / mice / secretory proteins
Research Abstract

When mouse embryonic stem cells (ES cells) are co-cultured on macrophage colony stimulating factor deficient OP9 stromal cells, hematopoietic cells can be differentiated via mesodermal cells. In order to clone the genes of extraccellular signaling proteins functioning during this process, cDNA cloning was carried out by using the day 5 differentiation induced cells. We utilized Signal Sequence Trap (SST) method which is an efficient cloning strategy for secretary proteins and type I transmembrane proteins and the method was innovated by our group.
As a result, one cDNA fragment which must contain authentic signal sequence. Full length cDNA was cloned by using the SST cDNA fragment as aprobe, this candidate gene turns out to be a murine homologue of the chicken gene whose expression is controlled by c-Myb but whose function is unknown yet. Now, the expression and the function of this gene is investigated.
Meanwhile, we consider it possible to clone the genes involved in the induction of early embryogenesis of mice by examining the similarities and the differences of the gene expression between ES cells and primordial germ cells. And we start to collect mRNA from purified primordial germ cells. We are planning to carry out differential display or SAGE (serial analysis of gene expression) to clone the genes.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] T.Era, T.Nakano, et al: "Thrombopoietin enhances proliferation and differentiation of murine yolk sac erythroid progenitors." Blood. 89. 1207-1213 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Aoki, T.Nakano, et al: "Induction of Bip mRNA upon programmed cell death of differentiated PC12 cells as well as rat sympathetic neurons." J Biochem. 121. 122-127 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.L.de la Pompa, T.Nakano, et al: "Conservation of the Notch signalling pathway in mammalian neurogenesis." Development. 124. 1139-1148 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Aoki, T.Nakano, et al: "Rat TAFII31 gene is induced upon programmed cell death in differentiated PC12 cells deprived of NGF." BBRC. 234. 230-234 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yamane, T.Nakano, et al: "Development of osteoclasts from embryonic stem cells through a pathway that is c-Fms,but not c-Kit dependent." Blood. 90. 3516-3573 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.R.Carlyle, T.Nakano, et al: "Identification of a novel developmental stage marking lineage commitment of progenitor thymocytes." J.Exp.Med.186. 173-182 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Era, T.Takahashi, K.Sakai, K.Kawamura, T.Nakano.: "Thrombopoietin enhances proliferation and differentiation of murine yolk sac erythroid progenitors." Blood. 89. 1207-1213 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Aoki, T.Koike, T.Nakano, K.Shibahara, S.Kondo, H.Kikuchi, T.Honjo: "Induction of Bip mRNA upon programd cell death of differentiated PC12 cells as well as rat sympathetic neurons." J.Biochem.121. 122-127 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.L.de la Pompa, A.Wakeham, K.M.Correia, E.Samper, S.Brown, R.J.Aguilera, T.Nakano, T.Honjo, T.W.Mak, J.Rossant, R.A.Conlon.: "Conservation of the Notch signalling pathway in mammalian neurogenesis." Development. 124. 1139-1148 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Aoki, T.Koike, T.Nakano, K.Shibahara, H.Nishimura, H.Kikuchi, T.Honjo: "Rat TAFII31 gene is induced upon programd cell death in differentiated PC12 cells deprived of NGF." BBRC. 234. 230-234 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.R.Carlyle, A.M.Michie, C.Furlonger, T.Nakano, M.J.Lenardo, C.J.Paige, J-C,Zuniga-Pfucker: "Identification of a novel developmental stage marking lineage commitment of progenitor thymocytes." J.Exp.Med.186. 173-182 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Yamane, T.Kunisada, H.Yamazaki, T.Era, T.Nakano, S-I.Hayashi: "Development of osteoclasts from embryonic stem cells through a pathway that is c-Fms, but not c-Kit dependent" Blood. 90. 3516-3523 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Era, T.Nakano, et al: "Thrombopoietin enhances proliferation and differentiation of murine yolk sac erythroid progenitors." Blood. 89. 1207-1213 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Aoki, T.Nakano, et al: "Induction of Bip mRNA upon programmed cell death of differentiated PC12 cells as well as rat sympathetic neurons." J Biochem. 121. 122-127 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.L.de la Pompa, T.Nakano, et al: "Conservation of the Notch signalling pathway in mammalian neurogenesis." Development. 124. 1139-1148 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Aoki, T.Nakano, et al: "Rat TAFll31 gene is induced upon programmed cell death in differentiated PC12 cells deprived of NGF." BBRC. 234. 230-234 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Yamane, T.Nakano, et al: "Development of osteoclasts from embryonic stem cells through a pathway that is c-Fms,but not c-Kit dependent." Blood. 90. 3516-3573 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.R.Carlyle, T.Nakano, et al: "Identification of a novel developmental stage marking lineage commitment of progenitor thymocytes." J.Exp.Med.186. 173-182 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Nakano,H.Kodama,T.Honjo: "In vitro development of primitive and definitive erythrocytes from different precursors." Science. 272. 722-724 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Tashiro,T.Nakano,T.Honjo: "Signal sequence trap : expression cloning methods for secreted proteins and type1 transmembrane proteins." Methods in Molecular Biology. 69. 203-219 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Hamada,T.Nakano,et al.: "Isolation and characterization of a novel soluble factor gene : a contibutional trial for resolution of intercellular signal transduction." Genomics. 37. 273-280 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Era,T.Takahashi,K.Sakai K.Kawamura,T.Nakano: "Theombopoietin enhances proliferation and differentiation of murine yolk sac progenitors" Blood. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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