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Molecular Mechanism of Mitoshondrial Protein Import in Higher Animals

Research Project

Project/Area Number 08457040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKumamoto University

Principal Investigator

MORI Masataka  Kumamoto University, School of Medicine, Professor, 医学部, 教授 (40009650)

Co-Investigator(Kenkyū-buntansha) TERADA Kazutoyo  Kumamoto University, School of Medicine, Assistant Professor, 医学部, 助手 (00253724)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1996: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsMitochondria / Protein import / Tom20 / Ornithine transcarbamylase / Aspartate aminotransferase / Serine : pyruvate aminotransferase / Zonation / DnaJホモログ / HSDJ / Hsc70
Research Abstract

Most mitochondrial proteins are synthesized in the cytosol and transported into the mitochondria via Tom complex on the outer membrane. We analyzed the roles of Tom20 using in vitro import system and cultured cell system. Import of pre-ornithine transcarbamylase (pOTC), pre-aspartate aminotransferase (pAAT) and pre-serine : pyruvate aminotransferase (pSPT) was strongly inhibited by soluble domain of Tom20. Import of these precursors was also inhibited by an antibody against Tom20. However, the degree of inhibition differed from one precursor to another. When pOTC is expressed in COS-7 cells, pOTC appeared first and then imported into the mitochondria and processed to the mature form. When Tom20 was co-expressed with pOTC,mitochondrial import and processing of pOTC was retarded. On the other hand, co-expression of soluble Tom20 had little effect. All these results indicate that Tom20 is involved in mitochondrial protein import and that the requirement of Tom20 differs among the precursor proteins.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Terada, K.et al.: "Participation of the import receptor Tom20 in protein import into mammalian mitochondria : Analyses in vitro and in cultured cells" FEBS Letters. 403・3. 309-312 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yano, M.et al.: "Visualization of mitochondrial protein import in cultured mammalian cells with green fluorescent protin and effects of overexpression of the human import receptor Tom20" The Journal of Biological Chemistry. 272・13. 8459-8465 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kanazawa, M.et al.: "HSDJ,a human homolog of DnaJ,is involved in protein import into mitochondria" The Journal of Biochemistry. 121・5. 890-895 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terada, K.et al.: "The human DnaJ homologue dj2 facilitates mitochondria protein import and luciferase refolding" The Journal of Cell Biology. 139・5. 1089-1095 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terada, K.et al.: "Participation of the import receptor Tom20 in protein import into mammalian mitochondria : Analyzes in vitro and incultured cells." FEBS Letters. 403(3). 309-312 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yano, M.et al.: "Visualization of mitochondrial protein import in cultured mammalian cells with green fluorescent protein and effects of overexpression of the human import receptor Tom20." The Journal of Biological Chemistry. 272(13). 8459-8465 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kanazawa, M.et al.: "HSDJ,a human homolog of DnaJ,is involved in protein import into mitochondria." The Journal of Biochemistry. 121(5). 890-895 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terada, K.et al.: "The human DnaJ homologue dj2 facilitates mitochondria protein import and luciferase refolding." The Journal of Cell Biology. 139(5). 1089-1095 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terada,K. et al.: "Participation of the import receptor Tom20 in protein import into mammalian mitochondria : Analyses in vitro and in cultured cells." FEBS Letters. 403(3). 309-312 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yano,M. et al.: "Visualization of mitochondrial protein import in cultured mammalian cells with green fluorescent protein and effects of overexpression of the human import receptor Tom20." The Journal of Biological Chemistry. 272(13). 8459-8465 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kanazawa,M. et al.: "HSDJ, a human homolog of DnaJ, is involved in protein import into mitochondria." The Journal of Biochemistry. 121(5). 890-895 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Terada,K., et al.: "The human DnaJ homologue dj2 facilitates mitochondrial protein import and luciferase refolding." The Journal of Cell Biology. 139(5). 1089-1095 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Terada, K., et al.: "Requirement of heat shok cognate 70 pretein for mitochondrial import varics among proccursor protcins and depending precursor length" Mol. Cell. Biol.16. 6103-6109 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Gotoh, T., et al.: "Molecular cloning of cDNA for nonhrpatic mitochondiral arginase (arginase II) and comparison of is induction with nitric oxide synthases in a murine macrophatge-like cell line" FEBS Lett.395. 119-122 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kanazawa, M., et al.: "HSDJ, a human homolog of DnaJ, is farnecslyted and is involved in protein import into mitochondria" J. Biochem.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Yano, M., et al.: "Visualization of mitochondrial import incultures mammalian cells with green fluoreseect protcin andeffects of overezpressionof the import recrptor Tom 20" J. Biochem.(in press).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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