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Study of the molecular mechanism of insulin exocytosis by SNARE hypothesis

Research Project

Project/Area Number 08457057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKyorin University school of Medicine

Principal Investigator

NAGAMATSU Shinya  Kyorin Univ.Sch.of Med., Dept.of Biochem., Associate Professor, 医学部, 助教授 (80231489)

Co-Investigator(Kenkyū-buntansha) SAWA Hiroki  Kyorin Univ.Sch.of Med., Dept.of Neurosurgery, Assintant Professor, 医学部, 講師 (80135912)
WATANABE Takashi  Kyorin Univ.Sch.of Med., Dept.of Clinical Pthology, Associate Professor, 医学部, 助教授 (00191768)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1998: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsSyntaxin / SNARE / Exocytosis / Pancreatic beta cell / SNAP / Diabetes Medllirus / Pancreatic βcell / インスリン分泌 / 膵β細胞 / NSF / 調節性分泌機構 / 構成性分泌機構 / 膵β細部
Research Abstract

The molecular mechanism of insulin exocytotic process in pancreatic beta cells is still unknown. Recently ; the fundamental components of the machinery for docking/fusion process have been revealed. These components include NSF, SNAP, and SNAP receptors. In the present project, we studied the functional roles of these components in insulin exocytotic process, and further examined the relationship between SNAP receptors and diabetes mellitus. We summarized the points of this research project as follows.
1) Syntaxin lA and SNAP-25 were expressed in pancreatic p cells and the biosynthesis was regulated by glucose.
2) alpha-SNAP plays a crucial role in glucose-stimulated insulin exocytosis via large dense core vesicles, but not GABA release via synaptic-like microvesicles inpancreatic beta cells.
3) alpha-SNAP functions in insulin exocytosis from mature, but not immature insulin secretory granules.
4) Expression of t-SNAREs was decreased in diabetic GK rat islets. Restoration of decreased t-SNARE protein to the normal level improved the impaired insulin release in diabetic GK rat islets.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] S.Nagamatsu, et.al: "α-soluble N-ethylmaleimide-sensitive factor attachment protein is expressed in pancreatic β cells and functions in insulin, but not gamma-aminobutyric acid-secretion" J.Biol.Chem.274. 8053-8060 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Watanabe, S.Nagamatsu, et al.: "Co-iocalization of GLUT3 and choline acetyltransferase immunoreactivity in the rat retina" Biochem. Biophys. Res.Commun.(印刷中). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Okuda et al.: "Forebrain expression of GLUT3 mRNA in 6-hydroxydopamine-lesioned rats" Biogemic Amines. 14. 581-590 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Nagamatsu et al.: "Overexpressed syntaxin 1A/HPC-1 inhibits insulin secretion via regulated pathway, but does not influence glucose metabolism and intracellular Ca2+ in insulinoma cell line βTC3 cells." Biochem. Biophys. Res.Commun.231. 89-93 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Nagamatsu et al: "Non-Functional role of syntaxin 2 in insulin exocytosis by pancreatic β cells." Cell Biochem. Function. 15. 237-242 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Nagamatsu, et al: "Syntaxin, but not soluble NSF attachment protein(SNAP), biosynthesis by rat pancreatic islets is regulated by glucose in parallel with proinsulin biosynthesis." Diabetologia. 40. 1396-1402 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 永松 信哉: "医学のあゆみ" 医歯薬出版株式会社, 4ページ (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 永松 信哉: "分子糖尿病学の進歩" 金原出版, 7ページ (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagamatsu S,Watanabe T,Nakamichi Y,Yamamura C,Tsuzuki K,and Matsushima S: "alpha-Soluble N-ethylmaleimide-sensitive factor attachment protein is expressed in pancreatic beta cells and function in insulin, but not gamma-aminobutyric acid secretion" J.Biol.Chem.274. 8053-8060 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagamatsu S., Nakamichi Y., Katahira H.: "Syntaxin, but not soluble NSF attachment protein (SNAP), biosynthesis by rat pancreatic islets is regulated by glucose in parallel with proinsulin biosynthesis" Diabetologia. 40. 1396-1402 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagamatsu S., Nakamichi Y., Yamaguchi K., Sawa H., and Akagawa K.: "Overexpressed syntaxin 1A/HPC-1 inhibits insulin secretion via regulated pathway, but does not influence glucose metabolism aud intracellular Ca^<2+> in insulinoma cell line betaTC3 cells." Biochem.Biophys.Res, Commun. 231. 89-93 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagamatsu S,Fujiwara T,Nakamichi Y,Watanabe T,Katahira H,Sawa H,Akagawa K: "Expression and functional role of syntaxin 1/HPC-1 in pancreatic beta cells." J.Biol.Chem. 271. 1160-1165 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Nagamatsu,et.al: "α-soluble N-ethylmaleimide-sensitive factor attachment protein is expressed in pancreatic β cells and functions in insulin,but not gamma-aminobutyric acid secretion" J.Biol.Chem.274. 8053-8060 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Watanabe,S.Nagamatsu,et al.: "Co-localization of GLUT3 and choline acetyltransferase immunoreactivity in the rat retina" Biochem.Biophys.Res.Commun.(印刷中). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Okuda et al.: "Forebrain expression of GLUT3 mRNA in 6-hydroxydopamine-lesioned rats" Biogemic Amines. 14. 581-590 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 永松 信哉: "医学のあゆみ" 医歯薬出版株式会社, 4 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 永松信哉: "SNARE仮説によるインスリン分泌の分子機構" Diabetes Journal. 25. 53-59 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagamatsu S.et.al.: "Overexpressed Syntaxin 1A/hpc1 inhibits insulin secretion." Biochem.Biophys.Res.Commun.231. 89-93 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagamatsu S.et.al.: "Non-functional role of syntaxin2 in insulin exocytosis." Cell Biochem.Funct.15. 237-242 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagamatsu S.et.al.: "Syntaxin,but not SNAP,biosynthesis by rat pancreatic islets." Diabetologia. 40. 1396-1402 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 永松信哉: "分子糖尿病の進歩-基礎から臨床まで-" 金原出版, 8 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shinya Nagamatsu et.al.: "Rat C6 glioma cell growth is related to glucose transport and metabolism" Biochem.J.319. 477-482 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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