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Expression of new cell-cell adhesion molecule and mucin genes in cancers : its relationship with invasion or metastasis of cancers

Research Project

Project/Area Number 08457064
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionKagoshima University

Principal Investigator

YONEZAWA Suguru  Faculty of Medicine Kagoshima University, Associate Professor, 医学部, 助教授 (10175002)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Ikuro  Faculty of Medicine Kagoshima University, Professor, 医学部, 教授 (20082282)
MATSUSHITA Yoshifumi  Faculty of Medicine Kagoshima University, Research Associate, 医学部, 助手 (90244227)
SATO Eiichi  Faculty of Medicine Kagoshima University, Professor, 医学部, 教授 (60004579)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsThrombomodulin / Squamous cell carcinoma / Adenocarcinoma / MUC1 mucin / MUC2 mucin / MUC5AC mucin / Sialic acid / Prognosis of patient carrying cancer / ムチン / 食道癌 / 消化器癌 / in situ hybridization / 肺癌 / ムチン抗原
Research Abstract

1. Squamous cell carcinoma
(1) Esophageal squamous cell carcinoma (S CC) cell lines produced the same thrombomodulin (TM) molecule as human endothelial cell line. The TM-low-expressing esophageal SCC cells expressed high invasiveness than TM-high-expressing esophageal SCC cells.
(2) In SCCs of oral cavity and lung, the tumor cells that were positive for TM expression were significantly rarer in the lymph node metastatic lesions than in the primary tumors.
(3) In SCCs of pharynx and larynx, and of esophagus, sialylated MUC1 mucin (DF3 antigen) was an effective tumor marker. The expression of sialyl oligosaccharides of DF3 antigen was related to poor prognosis in esophageal SCCs.
2. Adenocarcinoma
(1) Even in the expansive growth type tumors of pancreas and intrahepatic bile duct, invasive growth areas of the tumors acquire a characteristic of MUC1 mucin expression that is usually seen in the invasive growth type tumors.
(2) In situ hybridization study disclosed that MUC2 mucin expression in m … More ost of expansive growth type tumors of pancreas and intrahepatic bile duct was regulated by MUC2mRNA.Patients with positive MUC2mRNA expression in the tumors showed significantly better survival than those with negative MUC2mRNA expression in the tumors.
(3) Patterns of MUC5AC (gastric type) and MUC2 (intestinal type) gene expression in "mucin producing tumors of the pancreas (MPTs)", which usually reveal less invasive growth, showed variety among the subtypes of MPTs.
(4) In the tumors of pancreas and intrahepatic bile duct, sialic acid epitope of MUC1 mucin seems to play an important role in the invasion growth.
(5) In carcinomas of ampulla of Vater and stomach, the patients with MUC1(+) expression showed poorer outcome than those with MUC1(-) expression, whereas the patients with MUC2(+) expression showed more favorable outcome than those with MUC2(-) expression.
(6) Significant reduction of pericryptal fibroblast sheath in colorectal epithelial neoplasms reflects progression in the adenoma-carcinoma sequence. Less

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (65 results)

All Other

All Publications (65 results)

  • [Publications] Kitamura H.,et al.: "Expression of mucin carbohydrates and core proteins in carcinomas of the ampulla of Valter: Their relationship to prognosis" Japanese Journal of Cancer Research. 87. 631-640 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seko A.,et al.: "Quantitative differences in GlcNAc:β1→3and GlcNAc:β1→4 galactosyltransferase activities between human colonic adenocarcinomas and normal colonic mucosa" Cancer Research. 56. 3468-3473 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Itoh T.,et al.: "Expression of mucin antigens and Lewis X-related antigens in carcinomas and dysplasia of the pharynx and larynx" Pathology International. 46. 646-655 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tabata M.,et al.: "An immunohistochemical study of thrombomodulin in oral squamous cell carcinoma and its association with invasive and metastatic potential" Journal of Oral Pathology & Medicine. 26. 258-264 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakamoto H.,et al.: "Mucin antigen expression in gastric carcinomas of young and old adults" Human Pathology. 28. 1056-1065 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yonezawa S.,Sato E.: "Expression of mucin antigens in human cancers and its relationship with malignancy potential" Pathology International. 47. 813-830 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsushita Y.,et al.: "A subcloned human esophageal squamous cell carcinoma cell line with low thrombomodulin expression showed increased invasiveness compared with a high thrombomodulin-expressing clone" Cancer Letters. 127. 195-201 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yonezawa S.,et al.: "MUC-1 mucin expression in invasive areas of intraductal papillary mucinous tumors of the pancreas" Pathology International. 48. 319-322 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Utsunomiya T.,et al.: "Expression of MUC1 and MUC2 mucins in gastric carcinomas:Its relationship with the prognosis of the patients" Clinical Cancer Research. 4. 2605-2614 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yonezawa S.,et al.: "Gene expression of gastric type mucin(MUC5AC)in pancreatic tumors: Its relationship with biological behavior of the tumors: Its relationship with biological behavior of the tumors" Pathology International. 49. 45-54 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sagara M.,et al.: "Expression of mucin 1 (MUC1) in esophageal squamous cell carcinoma: Its relationship with prognosis of the patients" International Journal of Cancer. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Li A.,et al.: "Immunohistochemical analysis of pericryptal fibroblast sheath and proliferating epithelial cells in human colorectal adenomas and carcinomas with adenoma components" Pathology International. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kitamura H., Yonezawa S., Tanaka S., Kim Y.S., Sato E.: "Expression of mucin carbohydrates and core proteins in carcinomas of the ampulla of Vater : Their relationship to prognosis." Japanese Journal of Cancer Research. 87. 631-640 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Seko A., Ohkura T., Kitamura H., Yonezawa S., Sato E., Yamashita K.: "Quantitative differences in GlcNAc : beta1*3 and GlcNAc : beta1*4 galactosyltransferase activities between human colonic adenocarcinomas and normal colonic mucosa." Cancer Research. 56. 3468-3473 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Itoh T., Yonezawa S., Nomoto M., Ueno K., Kim Y.S., Sato E.: "Expression of mucin antigens and Lewis X-related antigens in carcinomas and dysplasia of the pharynx and larynx." Pathology International. 46. 646-655 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tabata M., Sugihara K., Yonezawa S., Yamashita S., Maruyama I.: "An immunohistochemical study of thrombomodulin in oral squamous cell carcinoma and its association with invasive and metastatic potential." Journal of Oral Pathology & Medicine. 26. 258-264 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakamoto H., Yonezawa S., Utsunomiya T., Tanaka S., Kim Y.S., Sato E.: "Mucin antigen expression in gastric carcinomas of young and old adults." Human Pathology. 28. 1056-1065 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yonezawa S., Sato E.: "Expression of mucin antigens in human cancers and its relationship with malignancy potential." Pathology International. 47. 813-830 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsushita Y., Yoshiie K., Imamura Y., Ogawa H., Imamura H., Takao S., Yonezawa S., Aikou T., Maruyama I., Sato E.: "A subcloned human esophageal squamous cell carcinoma cell line with low thrombomodulin expression showed increased invasiveness compared with a high thrombomodulin-expressing clone" Cancer Letters. 127. 195-201 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yonezawa S., Taira M., Osako M., Kubo M., Tanaka S., Sakoda K., Takao S., Aiko T., Yamamoto M., Irimura T., Kim Y.S., Sato E.: "MUC-1 mucin expression in invasive areas of intraductal papillary mucinous tumors of the pancreas." Pathology International. 48. 319-322 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Utsunomiya T., Yonezawa S., Sakamoto H., Kitamura H., Hokita S., Aiko T., Tanaka S., Irimura T., Kim Y.S., Sato E.: "Expression of MUC1 and MUC2 mucins in gastric carcinomas : Its relationship with the prognosis of the patients." Clinical Cancer Research. 4. 2605-2614 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yonezawa S., Horinouchi M., Osako M., Kubo M., Takao S., Arimura Y., Nagata K., Tanaka S., Sakoda K., Aiko T., Sato E.: "Gene expression of gastric type mucin (MUC5AC) in pancreatic tumors : Its relationship with bilological behavior of the tumors." Pathology International. 49. 45-54 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sagara M., Yonezawa S., Nagata K., Tezuka Y., Natsugoe S., Xing P.-X., McKenzie I.F.C., Aikou T., Sato E.: "Expression of mucin 1 (MUC1) in esophageal squamous cell carcinoma : Its relationship with prognosis of the patients." International Journal of Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Li A., Hasui K., Yonezawa S., Tanaka S., Sato E.: "Immunohistochemical analysis of pericryptal fibroblast sheath and proliferating epithelial cells in human colorectal adenomas and carcinomas with adenoma components." Pathology International. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsushita Y,et al.: "A subcloned human esophageal squamous cell carcinoma cell line with low thrombomodulin expression showed increased invasiveness compared with a high thrombomodulin-expressing clone" Cancer Letters. 127. 195-201 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yonezawa S,et al.: "MUC-1 mucin expression in invasive areas of intraductal papillary mucinous tumors of the pancreas" Pathology International. 48. 319-322 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Utsunomiya T,et al.: "Expression of MUC1 and MUC2 mucins in gastric carcinomas:Its relationship with the prognosis of the patients" Clinical Cancer Research. 4. 2605-2614 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Seko A,et al.: "Identification and characterization of N-acetylgkucosamine-6-0-sulfate-specific β1,4-galactosyltransferase in human colorectal mucosa" FEBS letters. 440. 307-310 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yonezawa S,et al.: "Gene expression of gastric type mucin(MUC5AC)in pancreatic tumors:Its relatiobship with bilological behavior of the tumors" Pathology International. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Fujikawa-Adachi K,et al.: "Identification of carbonic anhydrase IV and VI mRNA expression in human pancreas and salivary glands" Pancreas. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Sagara M,et al.: "Expression of mucin 1(MUC1)in esophageal squamous cell carcinoma:Its relationship with prognosis of the patients" International Journal of Cancer. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] 内倉 敬一郎,他: "胆管癌の肝側胆管切除断端における癌関連糖鎖抗原の発現と吻合部再発との検討" 日本消化器外科学会雑誌. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] Li A,et al.: "Immunohistochemical analysis of pericryptal fibroblast sheath and proliferating epithelial cells in human colorectal adenomas and carcinomas with adenoma compouents" Pathology International. (印刷中).

    • Related Report
      1998 Annual Research Report
  • [Publications] 米澤傑,他: "日本人の病気と病理学(病理と臨床 第17巻臨時増刊号) (分担執筆)" 文光堂 東京(印刷中),

    • Related Report
      1998 Annual Research Report
  • [Publications] Yonezawa S,et al.: "MUC2 gene expression is found in noninvasive tumors but not in invasive tumors of the pancreas and liver : Its close relationship with prognosis of the patients" Human pathology. 28. 344-352 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tabata M,et al.: "An immunohistochemical study of thrombomodulin in oral squamous cell carcinoma and its association with invasive and metastatic potential" Journal of Oral Pathology & Medicine. 26. 258-264 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Osaka M,et al.: "Immunohistochemical studies of mucin antigens in pancreas and intrahepatic bile-duct tumors" J.Hep.Bil.Pancr.Surg.4. 149-156 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakamoto H,et al.: "Mucin antigens expression in gastric carcinomas of young and old adults" Human Pathology. 28. 1056-1065 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsushita Y,et al.: "Carcohydrate antigens aberrantly expressed in colorectal carcinoma" Critical Reviews in ONCOLOGY/HEMTOLOGY. 25. 27-54 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Uehara F,et al.: "Glycopathological study of eyelid tumors and pseudotumors" Jpn.J.Ophthalmol.41. 362-369 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yonezawa S,et al.: "Expression of mucin antigens in human cancers and its relationship with malignant potential" Pathology International. 47. 813-830 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 米澤 傑、他: "ヒト消化器癌におけるムチン抗原の発現と癌の悪性度との関連性" 消化器癌の発生と進展. 9. 205-210 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 上原文行、他: "眼瞼脂漏性角化症の糖鎖組織化学的解析" 日本眼科学会雑誌. 101(5). 429-433 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 上原文行、他: "眼瞼惡性腫瘍のMUC2分布の免疫組織化学的検索" あたらしい眼科. 14(10). 1531-1534 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 上原文行、他: "眼瞼惡性腫瘍浸潤部におけるMUC1およびケラチン14分布の免疫組織化学的検索" 日本眼科学会雑誌. 101(11). 866-873 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yonezawa S,et al.: "MUC1 musin expression in invasive areas of intraductal papillary mucinous tumors of the pancreas" Pathology International. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsushita Y,et al.: "Subcloned human esophageal squamous cell carcinoma cell line with low thrombomodulin expression showed increased invasiveness compared to high thrombomodulin expressing clone" Cancer Letter. (印刷中).

    • Related Report
      1997 Annual Research Report
  • [Publications] 松下能文、他(分担): "[続]がんの浸潤・転移研究マニュアル-実験の手技と指針糖鎖抗原の免疫組織学的検出" がん転移研究会(金芳堂), 4 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松下能文(分担): "[続]がんの浸潤・転移研究マニュアル-実験の手法と指針CEAならびにCEA関連構造の免疫組織化学的・正化学的検出" がん転移研究会(金芳堂), 4 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kitamura H,et al.: "Expression of mucin carbohydrates and core proteins in carcinomas of the ampulla of Vater : Their relationship to prognosis." Japanese Jpurnal of Cancer Research. 87. 631-640 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Seko A,et al.: "Quantitative differences in GlcNAc : β1-3 and GlcNAc : β1-4 galactosyltransferase activities between human colonic adenocarcinomas and normal colonic mucosa." Cancer Research. 56. 3468-3473 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Itoh T,et al.: "Expression of mucin antigens and Lewis X-related antigens in carcinomas and dysplasia of the pharynx and larynx." Pathology International. 46. 646-655 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yonezawa S,et al.: "Expression of thrombomodulin in esophageal squamous cell carcinoma cell lines." Acta Histochemica et Cytochemica. 29. 109-113 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitamura H,et al.: "Differential mucin gene expression is associated withy methotrexate resistance of human colonic adenocarcinoma cell line HT29." European Journal of Cancer. 32A. 1788-1796 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 米澤傑: "消化器腫瘍におけるムチン抗原の発現" G.I.Research. 4. 380-388 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 米澤傑: "食道・肺の扁平上皮癌におけるトロンボモデュリンの発現とリンパ節転移に伴う発現の低下" リンパ学. 19. 59-64 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yonezawa S,et al.: "Mucin antigens expression and biological behavior of the tumors of pancreas and intrahepatic bile duct" Recent Advances in Gastroenterological Carcinogenesis. I. 559-564 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitamura H,et al.: "Expression of mucin carbohydrates and core proteins in carcinomas of the ampulla of Vater : Their relationship to the prognosis of the patients" Recent Advances in Gastroenterological Carcinogenesis. I. 629-633 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Itoh T,et al.: "Expression of mucin antigens and LeX-related antigens in carcinomas and dysplasia of the pharynx and larynx" Recent Advances in Gastroenterological Carcinogenesis. I. 785-789 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsushita Y,et al.: "Selection and characterization of thrombomodulin-expressing human esophageal squamous cell carcinoma. Its relation to invasiveness and possible function as sdhesion molecule" Recent Advances in Gastroenterological Carcinogenesis. I. 923-928 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yonezawa S,et al.: "Expression of thrombomodulin in esophageal squamous cell carcinoma and its relationship to lymph node metastasis" Recent Advances in Gastroenterological Carcinogenesis. I. 929-933 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsushita Y,et al.: "Carcohydrate antigens aberrantly expressed in colorectal carcinoma." Critical Reviews in ONCOLOGY/HEMATOLOGY. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 松下能文、他: "糖鎖抗原の免疫組織学的検出" 続・転移研究マニュアル. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Yonezawa S,et al.: "MUC2 gene expression is found in nonivasive tumors but not in invasive tumors of the pancreas and liver : Its close relationship with prognosis of the patients." Human Pathology. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakamoto H,et al.: "Mucin antigens expression in gastric carcinomas of young and old adults." Human Pathology. (印刷中).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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