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Establishment of in vitro replication of hepatitis C virus and its basic application

Research Project

Project/Area Number 08457067
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionNihon University School of Medicine

Principal Investigator

UCHIDA Toshikazu  Nihon Univ.School of the Asso.Pro., 医学部, 助教授 (80060078)

Co-Investigator(Kenkyū-buntansha) SUGITANI Masahiko  Nihon Univ.Assit.Pro., 医学部, 講師 (40187654)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1997: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
Keywordshepatitis / hepatitis virus / silent hepatitis B / HBsAg / transfection / B型肝炎 / サイレントB / 肺炎ウイルス / C型ウイルス / 肝発癌
Research Abstract

We have discovered the fact that hepatitis C virus (HCV) is coinfected with serum marker-negative (HBsAg-negative ; silent) hepatitis B virus (HBV) in a high frequency (probably always). This high frequent coinfection does not seem to be incidental due to the same route of infection of these two viruses but means that one virus is required for the infection and replication of the other virus. If so, the silent HBV may be required for the HCV, because there are many hepatitis patients infected with silent HBV alone. Therefore, we have cloned the full-genome silent HBVDNA and produced its head-to-tail dimer for replication. The silent HBVDNA and HCVRNA was cotransfected in vitro into established human hepatocellular carcinoma cells, termed HuH-7 cells, and subsequently the HCVRNA secreted into supernatants were quantified. The control consisted of a transfection HCVRNA alone and a cotransfection of wild-type (HBsAg-positive) HBVDNA and HCVRNA.As a result, the silent HBVDNA promoted the secretion of HCVRNA up to 5 times more than that of HCVRNA transfection alone. Conversely, the wild HBVDNA suppressed the secretion of HCVRNA up to 8 times than HCVRNA transfection alone. The latter phenomenon is well observed as an intereference of two viruses infected into a single cell. In contrast, the silent HBV may promote the replication and expression of HCVRNA and this fact will provide the important basis of the vitro replication of HCV.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Uchida T, Saito T, Shinzawa H: "Mutations of the X region of hepatitis B virus and their clinical implications" Pathol Int. 47. 183-193 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uchida T, Kaneita Y, et al.: "Hepatitis C is frequently coinfected with serum marker-negative hepatitis B virus" J Med Virol. 52. 399-405 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamagoe S, Akasaka T, Uchida T, et al.: "Expression of a neutrophil chemotactic protein LECT2 in human hepatocytes revealed by immunochemnical studies using polyclonal and monoclonal antibodies.." Biochem Biophys Res Commun. 237. 116-120 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Li T-C, Yamakawa Y, Uchida T, et al.: "Expression and self-assembly of empty virus-like particles of hepatitis E virus" J Virol. 71. 7207-7213 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uchida T,Saitoh T,Shinzawa: "Mutations of the X region of hepatitis B virus and their clinical implications." Pathol Int. 47. 183-193 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Uchida T,Kaneita Y,Gotoh K,Kanagawa H,Kouyama H,Kawanishi T,Mima S.: "Hepatitis C is frequently coinfected with serum marker-negative hepatitis B virus : probable replication promotion of the former by the latter as demonstrated by in vitro cotransfection." J Med Virol. 52. 399-405 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamagoe S,Akasaka T,Uchida T,Hachiya T,Okabe T,Yamakawa Y,Arai T,Mizuno S,Suzuki K: "Expression of a neutrophil chemotactic protein LECT2 in human hepatocytes revealed by immunochemical studies using polyclonal and monoclonal antibodies to a recombinant LECT2" Biochem Biophys Res Commun. 237. 116-120 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Li T-C,Yamakawa Y,Suzuki K,Tatsumi M,Razak M,Uchida T,Takeda N,Miyamura, T.: "Expression and self-assembly of empty virus-like particles of hepatitis E virus." J Virol. 71. 7207-7213 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shrestha SM,Okuda K,Uchida T,et al: "Endemicity and clinical picture of liver disease due to obstruction of the hepatic portion of the inferior vena cava in Nepal" J.Gastroenterol.Hepatol.11. 170-179 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Uchida T,Saitoh T,Shinzawa H: "Mutations of the X region of hepatitis B virus and their clinical implications." Pathol.Int.47. 183-193 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Uchida T,Kaneita Y,Gotoh K,et al.: "Hepatitis C is frequently coinfected with serum markernegative hepatitis B virus" J.Med.Virol.52. 399-405 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamagoe S,Akasaka T,Uchida T,et al.: "Expression of a neutrophil chmotatic protein LECT2 in human hepatocytes revealed by immunohistochemical studies using polyclonal and monoclonal antibodies" Biochem.Biophys.Res.Commun.237. 116-120 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Li T-C,Yamakawa Y,Uchida T,et al.: "Expression and self-assembly of empty virus-like particles of hepatitis C virus" J.Virol.71. 7207-7213 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Uchida et al.: "Mutationo of the X region and their chinical implicatcion" Pathol Int. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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