| Project/Area Number |
08457086
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
|
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| Research Institution | Nagoya University |
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Principal Investigator |
OHTA Michio School of Medicine Prof., 医学部, 教授 (20111841)
|
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| Co-Investigator(Kenkyū-buntansha) |
HORII Toshinobu School of Medicine Assistant Prof., 医学部, 助手 (80283430)
SHIBAYAMA Keigo School of Medicine Assistant Prof., 医学部, 助手 (50283437)
大須賀 伸二 名古屋大学, 医学部, 助手 (00262907)
荒川 宜親 名古屋大学, 医学部, 助教授 (10212622)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | cereulide / Bacillus cereus / foodborne illness / toxin / ionophore / liver failure / apotosis / mitochondria / cereulide / 脂肪変性 / K^+ イオノフォア |
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| Research Abstract |
Cereulide is the causative toxin of the emetic type foodborne illness caused by Bacillus cereus. Our previous studies exhibited that it is a cyclic depsipeptide and a potassium ionophore and that it induces mitochondrial swelling in cultured HEp-2 cells. This toxin was associated with fulminant liver failure in a human case. Chemically synthesized cereulide had the same emetic effect as purified cereulide. BALB/c mice were i.p. injected with various dosed of synthetic or purified cereulide and the development of histopathological changes as well as physiological effects were examined. Hepatocytes showed the abnormality of the mitochondria such as swelling and loss of cristae. Dose-dependent increase of small fatty droplets in the degenerated hepatocytes was also observed. These microsteatotic hepatocytes were distributed in the pericentral area and spread to the intermediate and periportal areas. In the cases of lethal doses, massive degeneration of hepatocytes was prominent. These fatal lesions included massive microsteatosis and apotosis of hepatocytes. Other organs of these mice had no apparent specific pathological changes.
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