| Project/Area Number |
08457096
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Mie University |
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Principal Investigator |
ITO Yasuhiko Mie university Faculty of Medicine Professor, 医学部, 教授 (00022872)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIO Machiko Mie university Faculty of Medicine Assistant Professor, 医学部, 助手 (70156040)
KAWANO Mitsuo Mie university Faculty of Medicine Lecture, 医学部, 講師 (00234097)
TSURUDOME Masato Mie university Faculty of Medicine Associate Professor, 医学部, 助教授 (50159042)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥8,500,000 (Direct Cost: ¥8,500,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | CELL FUSION / PARAMYXOVIRUS / FRP-1 / HIV / Cell Fusion / CPE |
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| Research Abstract |
We analyzed the mechanisms of cell fusion, multinucleated giant cell formation and virus entry using paramyxoviruses and HIV.We found an interesting cell fusion induction system, in which fusion (F) protein of paramyxovirus alone can induce multinucleated giant cells. We also found that there were two kinds of F protein of SV 5, that is, one F protein of SV 5 has an ability for induction cell fusion without HN protein and other F protein can induce cell fusion by an assistance of HN protein. There are three amino acid differences between these F proteins, and we identified amino acid responsible for inducibility of cell fusion by F protein alone.
We previously found that virus-induced cell fusion is regulated by some host cell factors, Fusion Regulatory Factors (FRPs)-1 and -2. We isolated 8 monoclonal antibodies directed against FRP-1 molecules and clarified their abilities. Anti-FRP-1 antibodies can be classified into three groups, namely, cell fusion enhancing antibodies, cell fusion suppressing antibodies and antibody showing no activity. Anti-FRP-1 antibody, both cell fusion enhancing and suppressing antibodies, can stimulate intracellular signaling. However, we could not find the difference between these antidodies-induced signaling. We also isolate 19 monoclonal antibodies directed against murine FRP-1. Murine FRP-1 was found to be alloantigens and to be identical to Ly 10 alloantigens.
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