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Analysis of flavivirus-cross-reactive immune responses which contribute to the pathogenesis of dengue hemorrhagic fever

Research Project

Project/Area Number 08457100
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionNational Institute of Infectious Diseases (1998)
Kinki University (1996-1997)

Principal Investigator

KURANE Ichiro  National Institute of Infectious Diseases・Department of Virology I, Director, ウイルス第一部, 部長 (90278656)

Co-Investigator(Kenkyū-buntansha) TAKASAKI Tomohiko  National Institute of Infectious Diseases・Department of virology I, Senior Scien, ウイルス第一部, 主任研究官 (20221351)
正木 秀幸  近畿大学, 医学部, 助手 (90247982)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥8,000,000 (Direct Cost: ¥8,000,000)
Fiscal Year 1998: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsDengue virus / Japanese encephalitis virus / T lymphocyte / Dengue hemorrhagic fever / デンブウイルス
Research Abstract

The aim of the present researh is to elucidate the role of flavivirus-cross-reactive immune responses in the pathogenesis of dengue hemorrhagic fever. We analyzed T lymphocytes which were cross-reactive for dengue and Japanese encephalitis viruses (JEV), using murine and human lymphocytes. T lymphocytes obtained from JEV-immune donors responded to JEV, but also responded to dengue viruses to lower levels. JEV-reactive CD4+ T cell clones established from the same donors did not respond to dengue viruses ; however, some T cell clones responded to West Nile virus (WNV). This may partly due to higher levels of amino acid homology between JEV and WNV.These results also suggest that T lymphocytes cross-reactive for JEV and dengue viruses exist only at the low frequency. These T cell clones did not possess unique T cell receptor motifs.
We also analyzed murine T cell responses to JEV, in order to understand T cell responses cross-reactive for dengue and JEV.We analyzed induction of JEV-specific CD8+ cytotoxic T lymphocytes (CTL) in Balb/c mice, and attempted to determine epitope (s) recognized by specific CTL.JEV-specific CD8+CTL were induced by infection with JEV.There is one epitope on the envelope protein recognized by these CTL.The epitope is located between amino acids 60-68 on the envelope protein, and the recognition of this epitope is restricted by H-2Kd. We are analyzing flavivirus-cross-reactivity of these CTL, and determining other epitopes recognized by the CD8+CTL.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Hirokni Aihara: "Establishment and Characterization of Japanese encephalitis virus-specific human CD4^r T-cell clones : flavivirus-cross-reactivity,pretein recognition,and cytotoxic activity" Journal of Virology. 72. 8021-8036 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 倉根一郎: "デング熱とデング出血熱" Pharma Medica. 16(3). 55-60 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 倉根一郎: "デング熱・デング出血熱" カレントテラピー. 17. 131-135 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ichiro Kurane: "Dengue virus-specific T lymphocyte responses and role of T lymphocytes in the pathogenesis of dengue hemorrhagic fever" Japanese Journal of Tropical Medicine and Hygiene. 27. 81-83 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ken-ichiro Yamada: "Laboratory diagnosis of imported dengue cases" Japanese Journal of Tropical Medicine and Hygiene. 27. 75-77 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirokuni Aihara et al.: "Establishment and characterization of Japanese encephalitis virus-specific, human CD4+ T cell clones : flavivirus cross-reactivity, protein recognition and cytotoxic activity" Journal of Virology. 72. 8032-8036 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ichiro Kurane: "Dengue fever and dengue hemorrhagic fever" Pharman Medica.16. 55-60 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ichiro Kurane: "Dengue fever/Dengue hemorrhagic fever" Current Therapy. 17. 131-135 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ken Ichiro Yamada et al.: "Laboratory diagnosis of imported degue cases" Japanese Journal of Tropical Medicine and Hygiene.27. 81-83 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ichiro Kurane: "Dengue virus-specific T lymphocyte responses and the role of T lymphocytes in the pathogenesis of dengue hemorrhagic fever." Japanese Journal of Tropical Medicine and hygiene.27. 81-83 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirokuni Aihara: "Establishment and Characterization of Japanese encephalitis virus-speufic,Human CD4^+ T-cell clones : flavivirus-cross-reactivity,protein recognition,and cytotoxic activity" Journal of Virology. 72・10. 8032-8036 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 倉根一郎: "デング熱とデング出血熱" Pharma Medica. 16・3. 55-60 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 倉根一郎: "デング熱・デング出血熱" カレントテラピー. 17・2. 131-135 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ichiro Kurane: "Dengue virus-specific T lymphocyte responses and role of T lymphocytes in the pathogenesis of dengue homorrhagic fever" Japanese Journal of Tropical Medicine and Hygiene. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ken-ichiro Yamada: "Laboratory diagnosis of imported dengue cases" Japanese Journal of Tropical Medicine and Hygiene. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 倉根一郎: "デング出血熱/日本脳炎" 化学療法の領域. 13巻・S-1号. 167-173 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 倉根一郎, 小西英二: "日本脳炎ウイルス遺伝子を含む組み換えポックスウイルスに対するヒトT細胞免疫応答" Neuro・Infection. 2巻・1号. 141-142 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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