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Experimental Study on a new mechanism of allergic asthma-like-pathogenesis.

Research Project

Project/Area Number 08457143
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Public health/Health science
Research InstitutionNational Institute for Environmental Studies.

Principal Investigator

SAGAI Masaru  National Institute for Environmental Studies, Res.Team for Health Effects of Air Pollutants., Head of Res.Team., 地域環境研究グループ, 総合研究官 (80124345)

Co-Investigator(Kenkyū-buntansha) TAKANO Hirohisa  National Institute for Environmental Studies, Res.Team for Health Effects of Air, 地域環境研究グループ, 主任研究員 (60281698)
ICHINOSE Takamichi  National Institute for Environmental Studies, Res.Team for Health Effects of Air, 地域環境研究グループ, 主任研究員 (50124334)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥3,100,000 (Direct Cost: ¥3,100,000)
Keywordsasthma / diesel exhaust particles / Diesel exhaust / Eosinophil infiltration / globlet cells / airway hyperresponsiveness / IgG1 antibody / IgE antibody / デューゼル排気微粒子 / ディーゼル排気 / 好酸球浸潤 / 杯細胞増生 / IgG1抗体 / IgE抗体 / 気管支喘息 / ディーゼル排気微粒子(DEP) / 粘液過剰産生 / 気道過敏性 / IgG1 / IgE / サイトカイン
Research Abstract

We previously reported that there is a new mechanism of asthma-like pathogenesis via IgG1 antibody. To confirm this evidence, in the last year, we had compared the differences on expression of asthma-like-features such as 1) an infiltration of eosinophils into airway mucosa, 2) proliferation of goblet cells and 3) airway hyperresponsiveness by intratracheal instillation of diesel exhaust particles (DEP) and ovalbumin (OA) between BALB/c mouse with high ability of IgE production and C3H/He mouse with high ability of IgG1 production. We found that only C3H/He mouse had expressed astuma-like-features. This suggest that IgG1 antibody may play an important role on the expression of asthma-like-features.
In this year, we had carried out two experiments by intratracheal instillation of DEP and OA,and inhalation of diesel exhaust (DE) and OA to CH3/He mouse. In these exPeriment, first, OA+alum were administered intraperitoneally one time. Mice in DEP+OA group showed marked changes on the three features compared with the mice in OA group. IgE and IgG1 titers in the DEP+OA group were 2.2+0.8 and 47,000+6,400, respectively. Mice inhaled DE and OA also showed marked changes on the three features. However, the degree of the change was slightly moderate to compare with that in the mice administered DEP+OA.Although IgG1 titers were not different between DEP+OA and DE+OA groups, IgE titer in DE+OA group increased 15 times to compare with IgE titer in the DE+OA group.
From these results, there is no denying the involvement of IgE on the onset of asthma-like-features, because usage of alum is not suitable to make clear the involvement of only IgG1 antibody. Now, we reexamine an experiment without usage of alum on onset of asthma-like-features by inhalation of DE+OA.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] H.Takano, M.Sagai, et.al.: "Diesel exhaust particles.enhance antigen-induced airway inflammation and local cytokine express in mice." Am.J.Respir.Crit.Care Med.,. 156.36-42 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Ichinose, M.Sagai, et.al.: "Murine strain differences in allergic airway inflammation and immunoglobulin production by a combination of antigen and" Toxicology,. 122.183-192 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Miyabara, M.Sagai, et.al.: "Murine strain differences in airway inflammation and hyper-resonsiveness in mice." Europ.Respir.J.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Miyabara, M.Sagai, et.al.: "Diesel exhaust enhance allergic airway inflammation and hyper-responsiveness in mice." Am.J.Respir.Crit.Care Med.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 高野 裕久、市瀬 孝道、嵯峨井 勝:"ディーゼル排気による呼吸器障害の分子機構、" 分子呼吸器病、. 1.71-73 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 宮原 裕一、嵯峨井 勝:"空中浮遊微粒子の気道刺激性" アレルギー科、. 4.164-171 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takano, T.Yoshikawa, Y.Miyabara, K.Imaoka, T.Ichinose, & M.Sagai.: "Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine express in mice" Am.J.Respir.Crit.Care Med.156. 36-42 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Ichinose, H.Takano, Y.Miyabara, R.Yanagisawa, & M.Sagai.: "Murine strain differences in allergic airway inflammation and immunoglobulin production by a combination of antigen and diesel exhaust particles" Toxicology. 122. 183-192 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Miyabara, R.Yanagisawa, N.Shimojo, H.Takano, H.B.Lim, & M.Sagai.: "Murine strain differences in airway inflammation and hyperresonsiveness in mice" Europ.Respir.J.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Miyabara, H.Takano, T.Ichinose, H.B.Lim, M.Sagai: "Diesel exhaust enhance allergic airway inflammation and hyperresponsiveness in mice" Am.J.Respir.Crit.Care med.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Bayram, J.L.Devalia, R.J.SapsFord, T.Ohtoshi, Y.Miyabara, M.Sagai, R.J.Davies.: Am.J.Respir.Cell mol.Biol.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Takano, M.Sagai,et al.: "Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine express in mice." Am.J.Respir.Crit.Care Med.156. 36-42 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Ichinose, M.Sagai,et al.: "Murine strain differences in allergie airway inflammation and immunoglobulin production by a combination of antigen and" Toxicology. 122. 183-192 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Miyabara, M.Sagai,et al.: "Murine strain differences in airway inflammation and hyperresonsiveness in mice." Europ.Respir.J.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Miyabara, M.Sagai,et al.: "Diesel exhaust enhance allergic airway inflammation and hyperresponsiveness in mice." Am.J.Respir.Crit.Care Med.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 高野裕久、市瀬孝道、嵯峨井 勝: "ディーゼル排気による呼吸器障害の分子機構" 分子呼吸器病. 1. 71-73 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 宮原裕一、嵯峨井 勝: "空中浮遊微粒子の気道刺激性" アレルギー科. 4. 164-171 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Sagai,T.Ichinoseら: "Biological effects of diesel exhaust particles. III.phathogenesis of asthma" Free Radical Biol.Med.21,. 199-209 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Mori,M.Sagai et al.: "Inhibition of catalase activity in vitro by diesel exhaust particles" J.Toxicol.Environ.Health.47,. 125-134 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 嵯峨井勝ら: "酵素的反応によるディーゼル排気微粒子(DEP)からの活性酸素の生成" 磁気共鳴と医学. 8,. 101-104 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 嵯峨井勝ら: "ディーゼル排気微粒子(DEP)によるマウスの喘息様病態とEDRFとしてのNO" 日本胸部疾患学会誌. 34,. 213-218 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 嵯峨井勝ら: "ディーゼル排気微粒子(DEP)による呼吸器疾患〜気管支喘息,アレルギー性鼻炎と肺癌〜" 呼吸. 15,. 474-481 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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