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Research for human parvovirus and rheumatoid arthritis.

Research Project

Project/Area Number 08457151
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionTohoku University

Principal Investigator

SASAKI Takeshi  Tohoku Univ.Sch.Med.Professor, 医学部, 教授 (50110656)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Yuichi  Tohoku Univ.Sch.Med.Lecturer, 医学部・附属病院, 講師 (80261535)
SHIBATA Shinobu  Tohoku Univ.Sch.Med.Research associate, 医学部・附属病院, 助手 (00271932)
FUNATO Tadao  Tohoku Univ.Sch.Med.Lecturer., 医学部・附属病院, 講師 (70165455)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥5,800,000 (Direct Cost: ¥5,800,000)
Keywordshuman parvovirus / B19 / rheumatoid arthritis / arthritis
Research Abstract

The etiology of rhumatoid arthritis (RA) is unknown. We have shown the persistence of human parvovirus B19 (B19) for more than 6 years in a patients with B19 infections and also the cases, who developed into active RA after an acute B19 infection. B19-DNA and the B19 antigen VP-1 were detected in the synovial tissues in 23 out of 30 patients with RA,and infrequently in those with osteoarthrits (OA) and traumatic joints. The expression of VP-1 was specific in active synoviaI lesions of RA.In situ hybridization and immunohistochemical studies revealed that the target cells of B19 were macrophages, foIIicular dendritic cells, T Cells and B cells, but not synoviaI lining cells in the snovium. B19-negative bone marrow cells, anc macrophage ceIls became positive for the expression of VP-1, and more productive for IL-6 and tumor necrosis factor alpha (TNF alpha) when cocultured with RA synovial cells. The expression of VP-1 and the production of IL-6 and TNF alpha was significantly inhibited by the addition of neutralizing antibody for B19, suggesting that B19 detected in RA synovial cells are infective. These data indicate that B19 may be involved in the initiation and perpetuation of RA synovitis, leading to joint lesions.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Takahashi, Y・Sasaki, T.et al.: "Human parvovirus B19 in rtheumatoid arthrits." Inter.Rev.Immunol.17.2. in press (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Murai, C・Sasaki, T.et al.: "Rheumatoid arthritis after human Parvovirus B19 infection." Ann.Rheum.Dis.in press. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Munakata, Y・Sasaki, T.et al.: "Somatic mutation in autoantibody-associated VH genes of circulationg IgM+IgD+B cell." Eu.J.Immunol.in press. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Funato, T・Sasaki, T.et al.: "Reversal of cisplatin resistance in vivo by an anti-fos ribozyme." in vivo. 11. 217-220 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Bona, T, Sasaki.: "Pathogenic Autoimmune Reactions." Human Press Inc.in press, (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Bona, C.Murai, T.Sasaki: "The role of exogeneous stimulation in pathogenesis of autoimmune disease." Pathogenic Autoimmune Reactions. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.Murai, T.Sasaki, et al.: "Rheumatoid arthritis after human parvovirus B29 infection." Ann.Rheum.Dis.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Takahashi, T.Sasaki, et al.: "Human parvovirus B19 in rheumatoid arthritis." Inter, Rev, Immunol.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Munakata, T.Sasaki, et al.: "Somatic mutation in autoantibody-associated VH genes of circulating lgM+lgD+B cell." Eu.J.Immunol.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Takahashi, T.Sasaki, et al.: "Human parvovirus B19 and rheumatoid arthritis." Arthritis Rheum.40. S245 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Funato, T.Sasaki: "Reversal of cisplatin resistance in vivo by an anti-fos ribozyme." in vivo.11. 217-220 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Takahashi, U.Ikeda, T.Kasahara, S.Kitagawa, Y.Takahashi, et al.: "Activation of human monocytes for enhanced production of interleukin 8 during transendothelial migration in vitro." J.Clin.Immunol.17. 53-62 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Izumi, K.Hirai, M.Miyamasu, Y.Takahashi, et al.: "Expression and regulation of human monocyte chemotactic protein-1 by human eosinophils." Eur.J.Immunol.27. 816-824 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Nishimura, T.Akahoshi, M.Takahashi, K.Takagishi, M.Itoman, H.Kondo, Y.Takahashi, K.Yokoi, N.Hukaida, K.Matsushima: "Atteauation of monosodium mate crystal-induced arthritis in rabbits by newtralizing antibody against interleukin-8." J.Leukoc.Biol.62. 444-449 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] B.O.Lee, K.ishihara, T.Sasaki, et al.: "Elevated levels of the soluble from of bone marrow stomal cell antigen 1 in the sera of patients with severe rheumatoid arthritis." Arthritis and Rheum.39. 629-637 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Sasaki, Y.Takahashi, C.Murai, Y.Munakata, K.Ishii, K.Sugamura: "Persistently infected cells in human parvovirus B19 polyarthritis." Arthritis and Rheum.39. 147 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Wada, H.Yokoyama, S.Su, N.Mukaida, Y.Takahashi, T.Sasaki, et al.: "Monitoring urinary levels of monocyte chemotactic and activating factor reflects disease activity of lupus nephritis." Kidney Int.49. 761-767 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Swissa, A.Lerner, T.Sasaki, et al.: "The diverse pathogenic potential of aniti-DNA antibodies from various sources to induce experimental systemic lupus erythematosus." Pathobiology. 64. 32-39 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Takahashi, S.Kitagawa, J.Matsuyama, U.Ikeda, T.Kasahara, Y.Takahashi, et al.: "Human monocyte-endothelial cell interaction induce synthesis of granulocyte-macrophage colony stimulating factor." Criculation. 93. 1185-1193 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Suzuki, Y.Takahashi, H.Wataya, et al.: "Mechanism of neutrophil recruitment induced by interleukin-8 in chronic sinusitis." J.Allergy.Clin.Immunol.98. 659-670 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi Y, Sasaki T.et al.: "Human parvovirus B19 in rheumatoid arthrits." Inter.Rev.Immunol.17.2(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Murai C, SasakiT, et al.: "Rheumatoid arthritis after human Parvovirus B19 infection." Ann. Rheum.Dis.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Munakata Y, Sasaki T, et al.: "Somatic mutation in autoantibody-associated VH genes of circulationg IgM+IgD+B cell." Eu.J.Immunol.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Funato T, Sasaki T, et al.: "Reversal of cisplatin resistance in vivo by an anti-fos ribozyme." in vivo. 11. 217-220 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] C.Bona, T.Sasaki.: "Pathogenic Autoimmune Reactions." Human Press Inc.(in press), (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y. Takahashi, T. Sasaki: "Human parvovirus B19 in rheumatoid arthritis." Int. Rev. Immunol.14. (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] B. O. Lee, T. Sasaki, et al.: "Elevated levels of the soluble from of bone marrow atromal cell antigen 1 in the sera of patients with severe rheumatoid arthritis." Arthritis and Rheum.39. 629-37 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Sasaki, Y. Takabashi, C. Murai, Y. Munakata, K. Sugamura.: "Parsistently infected cells in human prvovirus B19 polyarthritis." Arthritis and Rheum.39. 147- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. Wada, T. Sasaki, et al.: "Monitoring urinary levels of monocyte chemotactic and activating factor reflects disease activity of lupus nephritis." Kidney Int.49. 761-7 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M. Swissa, T. Sasaki, et al.: "The diverse pathogenic potential of anti-DNA antibodies from various sources to induce experimental systemic lupus erythematosus." Pathobiology. 64. 32-9 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Takahashi, et al.: "Human monocyte-endothelial cell interaction induces synthesis of granulocyte macrophage colony stimulating factor." Circulation. 93. 1185-93 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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