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Supramolecular Assembly and Function of New Basement Membrane Collagen Molecules

Research Project

Project/Area Number 08457154
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionOkayama University

Principal Investigator

NINOMIYA Yoshifumi  Okayama University, Dept.of Mol.Biol.& Biochem.Professor, 医学部, 教授 (70126241)

Co-Investigator(Kenkyū-buntansha) MOMOTA Ryusuke  Okayama University, Dept.of Mol.Biol.& Biochem.Instructor, 医学部, 助手 (80263557)
OOHASHI Toshitaka  Okayama University, Dept.of Mol.Biol.& Biochem.Assistant Professor, 医学部, 助手 (50194262)
YOSHIOKA Hidekatsu  Okayama University, Dept.of Mol.Biol.& Biochem.Associate Professor, 医学部, 教授 (00222430)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥6,400,000 (Direct Cost: ¥6,400,000)
Keywordsbasement membrane / bidirectional promotor / gene expression / Alport syndrome / leiomyomatosis / type IV collagen / トポイソメラーゼ / 遺伝子発現調節 / ES細胞 / 超分子会合体 / 食道平滑筋腫
Research Abstract

To characterize the structure of mouse col4a6 gene, we screened mouse 129 genomic library and isolated a fragment of TSg6, approximately 18.3kb in size. Fine analysis of the genomic fragment and comparison of the structure to that of the cDNA revealed that alternative transcripts that are observed in the human gene do not exist when the mouse is transcribed. In order to create null mouse for col4a6 gene, we manipulated genomic fragment and inserted NcoR gene into the exon 2 of col4a6 gene. The vector was transfected into the R1-Es cells derived from 129v mouse and positive ES cells screened by G418. The positive ES cells were inserted into blastocysts, then the blastocysts were moved uterus of pseudopregnant mice. Chimera mice, heterozygotes and homozygotes are now being checked if any phenotypes are noticed. When homozygote mice were stained by a6 specific monoclonal antibodies, no positive signals was obtained from any tissues, indicating that a6 polypeptide is not expressed in the m … More ice.
While collagen IV molecules composed of alpha1 and alpha2 chains are broadly distributed, molecules comprising combinations of the other four chains, alpha3-alpha6, are important components of specialized basement membranes. The precise chain composition of triple-helical molecules assembled from the alpha3-alpha6 chains is not entirely clear, but it is hypothesized that alpha3-alpha5 chains and alpha5 and alpha6 chains form heterotrimeric molecules. Several pieces of evidence indicate that alpha3/alpha4/alpha5 molecules and alpha5/alpha6 molecules are components of the basement membrane network.
We identified an DL/AS deletion and determined the breakpoint wequences of the Japanese case. The results demonstrated that a deletion clininates the first coding exon of COL4A5 and the first two of COL4A6. They also showed that the breakpoints share the same sequence, which is in turn closely homologous to the consensuses of topoisomerases I and II.Additional DNA evidence suggestetd that the male patient is a somatic mosaic for the mutation. Immunohistochemical analysis using alpha chain-specific monoclonal antibodies supportedthis conclusion in that they revealed the absence of the alpha5 (IV) and alpha6 (IV) collagen chains in most but not all of basement membranes of the smooth muscle cell tumor. This study is greatly relevant to the understanding of DL pathogenesis and its etiology. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (94 results)

All Other

All Publications (94 results)

  • [Publications] Kay E.et al.: "Post-translational regulation of type I collagen in corneal endothelial cells." Invest.Ophthalmol.Vis.Sci.37. 11-19 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugihara K.et al.: "Experimental Anti-GBM glomerular nephritis induced in rats by immunization with syhthetic peptides based on six α chains of human type IV collagen." J.Pathol.178. 352-358 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ljubimov A.V.et al.: "Extracellular alterations in human corneas with bullous keratopathy." Invest.Ophthal mol.Vis.Sci.37. 997-1007 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rauf S.et al.: "Nephritogenicity and α-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane." Virchow Archiv. 428. 281-288 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Onoda K.et al.: "Inhibition of vascular contraction in rat experimental spasm model by intracisternal administration of preproendothelin-1 mRNA antisense oligoDNA." J.Neuroscience. 85. 846-852 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Onoda K.et al.: "Role of extracellular matrix in experimental vasospasm-Inhibitory effect of antisense oligonucleotide on collagen induction-." Stroke. 27. 2102-2109 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hino S.et al.: "Absence of α6(IV) collagen in kidney and skin of X-linked Alport syndrome." Pediatric Nephrology. 10. 742-744 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yanai H.et al.: "Regulation of interleukin-2 receptor gamma chain mRNA expression in human monocytic cell line THP-1." Acta Med.Okayama. 50. 145-150 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwata M.et al.: "The 160k α1(IV) chain,a short form of a tyep IV collagen polypeptide,of bovine lens capsule retains the NC1 domain." J.Biochem.120. 133-137 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ljubinov, A V.et al.: "Basement membrane abnormalities in human eye with diabetic retinopathy." J.Histochem.Cytochem.44. 1469-1479 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Megumi Kagawa, et al.: "Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport's syndrome." Nephrol.Dial.Transplant.12. 1238-1241 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sado Y.et al.: "Purification and characterization of human nephritogenic antigen that induces anti-GBM nephritis in rats." J.Pathol.182. 225-232 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Heidet L.et al.: "Diffuse leiomyomatosis associated with X-linked Alport syndrome : extracellular matrix study using immunohistochemistry and in situ hybridization." Lab.Invest.76(2). 233-243 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka K.et al.: "Differential expression of α1(IV),α2(IV),α5(IV),α6(IV) collagen chains in the basement membrane of basal cell carcinoma." Histochem.J.29. 563-570 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Endo C.et al.: "Time-dependent increase in syndecan-1 and fibroglycan messenger RNA expression in the infarct zone after experimentally induced myocardial infarction in rats." Pathophysiol Natural Hist.8. 155-161 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sumiyoshi H.et al.: "Ubiquitous expression of the α1(XIX) collagen gene(Col19a1) during mouse embryogenesis becomes restricted to a few tissues in the adult organism." J.Biol.Chem.272. 17104-17111 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Khaleduzzaman M.et al.: "Structure of the human type XIX collagen(COL19A1) gene that suggests it has arisen from an ancestor gene of the FACIT family." Genomics. 45. 304-312 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ono, S.et al.: "Three-dimensional analysis of vasospastic major cerebral arteries in rats examined by the corrosion cast technique." Stroke. 28(8). 1631-1637 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fleischmajer R.et al.: "There is temporal and spatial expression of α1(IV),α2(IV),α5(IV),α6(IV) collagen chains and β integrins during the development of the basal lamina in an “in bitro" skin model." J.Invest.Dermatol.109(4). 527-533 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirohata S.et al.: "Laminin α1,α2,α4 and β chain mRNA expression in mouse embryonic,neonatal and adult hearts." Jpn.Heart J.38(2). 281-289 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Olson M.W.et al.: "High affinity binding of Matrix metalloproteinase-9 to the pro-α2(IV) chain of collagen IV. Role in binding of zymogen to the surface of human breast epthelial MCF10A cells." J.Biol.Chem.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshikazu Sado.et al.: "Induction of anti-GBM nephritis in rats by recombinant α3(IV) and α4(IV)NC1 of type IV collagen." Kidney International. 53. 664-671 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] George E.Lees.et al.: "A model of autosomal recessive Alport syndrome in English cocker spaniel dogs." Kidney International. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ueki Y.et al.: "Topoisomerase I and II consensus sequences in a 17-kb deletion junction of the COL4A5 and COL4A6 genes and immunohistochemical analysis of esophageal leiomyomatosis" Am.J.Hum.Genet.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Gunwar S.et al.: "Glomerular Basement Membrane : Identification of a novel disulfide-crosslinked network of α3,α4 and α5 chains of type IV collagen and its implications for the pathogenesis" J.Biol.Chem.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Heidet, L.et al.: "Somatic deletion of the 5'ends of both the COL4A5 and COL4A6 genes in a sporadic leiomyoma of the esophagus." Am J.Pathol.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ono, S.et al.: "NF-kB decoy administration into the subarachnoid space as gene therapy for angiopathy." Human Gene Therapy. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Momota, R.et al.: "Two genes,COL4A3 and COL4A4 coding for the human α3(IV) and α4(IV) collagen chains are arranged head-to-head on chromosome 2q36." FEBS Letters. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wu, Y.-L.et al.: "cDNA sequence and expression of the Mouse α1(V) Collagen Gene (Col5a1)." Biochem.Biophys.Acta. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harvey, S.J.et al.: "The role of distinct type IV collagen networks in glomerular development and function." Kidney International. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seki.T.et al.: "Differential expression of type IV collagen isoforms,α5(IV) and α6(IV) chains,in basement membranes surrounding smooth muscle cells." Histochem.Cell Biol.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshikazu Sado et al.: "Organization and Expression of Basement Membrane Collagen IV Genes and their Roles in Human Disorders." J.Biochem.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 吉岡 秀克: "関節マーカー" 岩田久・新名正由, 14 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 二宮 善文: "細胞外マトリックス-臨床医学への応用" 渡辺明治、岡崎勲, 10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tryggvason K.: "Clinical Studies in Medical biochemistry" Glew R.H.and Ninomiya Y., 8 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshikazu Sado: "ECM-Cell Interaction : Molecules to diseases" Ohyama,Ninomiya,Olsen, (in press), (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Olsen B.R.: "Guide book to the extracellular matrix and adhesion proteins" Thomas Kreis and Ron Vale (in press), (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 二宮 善文: "標準分子医化学" 藤田道也, 12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 二宮 善文: "形態形成・分子メカニズム研究の最新技術" 永井教之, 6 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 二宮 善文: "領域別症候群シリーズ「先天代謝異常症候群(下巻)」" 北川照男(in press), (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kay E.P., GuX., Choi S.-H., and Ninomiya Y.: "Post-translational regulation of type I collagen in corneal endothelial cells." Invest Ophthalmol Vis Sci. 37. 11-19 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugihara K., Sado Y., Ninomiya Y., and Wada H.: "Experimental Anti-GBM glomerular nephritis induced in rats by immunization with synthetic peptides based on six alpha chains of human type IV collagen." J.Pathol.178. 353-358 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ljubimov A.V., Burgeson R.E., Butkowski R.J., Couchman J.R., WuR.-R., Ninomiya Y., Sado Y., Maguen E., Nesburn A.B., and Kenney M.C.: "Extracellular alterations in human corneas with bullous keratopathy." Invest.Ophthal mol.Vis.Sci.37. 997-1007 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Rauf S., kagawa M., Kishiro Y., Inoue S., Naito I., Oohashi T., Sugimoto M., Ninomiya Y., Sado Y.: "Nephritogenicity and alpha-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane." Virchow Archiv. 428. 281-288 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Onoda K,Ono S,Ogihara K,Shiota T,Asari S,Ohmoto T,and Ninomiya Y.: "Inihibition of vascular contraction in rat experimental spasm model by intracisternal administration of preproendothelin-1 mRNA antisense oligoDNA." J.Neuroscience. 85. 846-852 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Onoda K,Ono S,Ogihara K,Shiotai T,Shoji S,Ohmoto T,and Ninomiya Y: "Role of extracellular matrix in experimental vasospasm-Inhibitory effect of antisense oligonucleotid on collagen induction-." Stroke. 27. 2102-2109 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hino S,Takemura T,Sado Y,Kagawa M,Oohashi T,Ninomiya Y,and Yoshioka K: "Absence of alpha6 (IV) collagen in kidney and skin of X-linked Alport syndrome." Pediatric Nephrology. 10. 742-744 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yanai H,Yoshinno T,Takahashi K,Ninomiya Y,Akagi T.: "Regulation of interleukin-2 receptor gamma chain mRNA expression in human monocytic cell line THP-1." A cta Med Okayama. 50. 145-150 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwata M., Sado Y., Sasaki, T., Imamura, Y., Ninomiya, Y., and Hayashi, T.: "The 160k alpha1 (IV) chain, a short form of a tyep IV collagen polypeptide, of bovine lens capsule retains the NC1 domain." J.Biochem. 120. 133-137 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ljubinov, A V,Burgeson RE,Butkowski RJ,Couchman JR,Zardi L,Ninomiya Y,Sado Y,Huang Z-S,Nesburn AB,and Kenny MC.: "Basement membrane abnormalities in human eye with diabetic retinopathy." J.Histochem.Cytochem.44. 1469-1479 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Megumi Kagawa, Yumiko Kishiro, ichiro Naito, Tadashi Nemoto, Hiroshi Nakanishi, Yoshifumi Ninomiya, and Yoshikazu Sado.: "Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport's syndrome" Nephrol.Dial.Transplant.122. 1238-1241 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sado Y.Kagawa M., Kishiro Y., Naito I., Joh K.Ninomiya Y.: "Purification and characterization of human nephritogenic antigen that induces anti-GBM nephritis in rats." J.Pathol.182. 225-232 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Heidet L,Cai Y,Sado Y,Ninomiya Y,Thorner P,Guicharnaud L,Boye E,Chauvet V,Solal LC,Beziau A,Torres RG,Antignac C,Gubler MC.: "Diffuse leiomyomatosis associated with X-linked Alport syndrome : extracellular matrix study using immunohistochemistry and in situ hybridization." Lab Invest. 76. 233-243 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka K,Iyama K,Kitaoka K,Ninomiya Y,Oohashi T,Sado Y,and Ono T: "Differential expression of alpha1 (IV), 2 (IV), 5 (IV), 6 (IV) collagen chains in the basement membrane of basal cell carcinoma." Histochem.J. 29. 563-570 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Endo c, Kusachi, S., Ninomiya, Y., Yamamoto, K., Murakami, M., Murakami, T., Shinji, T., Koide, N., Kondo, J., and Tsuji, T.: "Time-dependent increase in syndecan-1 and fibroglycan messenger RNA expression in the infarct zone after experimentally induced myocardial infarction in rats." Pathophysiol Natural Hist.8. 155-161 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sumiyoshi H., Inoguchi K., Khaleduzzaman M., Ninomiya Y., and Yoshioka H.: "Ubiquitous expression of the alpha1 (XIX) collagen gene (Col19a1) during mouse embryogenesis becomes restricted to a few tissues in the adult organism." J.Biol.Chem.272. 17104-17111 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Khaleduzzaman M., Sumiyoshi H., Yasuyoshi U., Inoguchi K., Ninomiya Y., and Yoshioka H.: "Structure of the human type XIX collagen (COL19A1) gene that suggests it has arisen from an ancestor gene of the FACIT family." Genomics. 45. 304-312 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ono, S., Date I., Nakajima, M., Onoda, K., Ogihara, K., Shiota: "Three-dimensional analysis of vasospastic major cerebral arteries in rats examined by the corrosion cast technique." Stroke. 28. 1631-1637 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fleischmajer R,Kuhn K,Sato Y,MacDonald ED 2nd, Perlish JS,Pan TC,Chu ML,Kishiro Y,Oohashi T,Bernier SM,Yamada Y,Ninomiya Y.: "There is temporal and spatial expression of alpha1 (IV), alpha2 (IV), alpha5 (IV), alpha6 (IV) collagen chains and beta integrins during the development of the basal lamina in an "in vitro" skin model." J Invest Dermatol. 109. 527-533 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirohata S,Kusachi S,Kondo J,Sano I,Murakami M,Doi M,Ninomiya Y,Tsuji T: "Laminin alpha1, alpha2, alpha4 and beta chain mRNA expression in mouse embryonic, neonatal, and adult hearts." Jpn Heart J. 38. 281-289 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Olson M.W., Toth M., Gervasi D.C., Sado Y., Ninomiya Y., and Fridman R.: "High affinity binding of Matrix metalloproteinase-9 to the pro-alpha2 (IV) chain of collagen IV.Role in binding of zymogen to the surface of human breast epthelial MCF10A cells." J.Biol.Chem.(in press.). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshikazu Sado, Ariel Boutaud, Megumi Kagawa, Ichiro Naito, Yoshifumi Ninomiya, and Billy G.Hudson.: "Induction of anti-GBM nephritis in rats by recombinant alpha3 (IV) and alpha4 (IV) NC1 of type IV collagen." Kidney International. 53. 664-671 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] George E.Lees, Gayman Helman, Clifford E.Kashtan, Alfred F.Michael, Linda D.Homco, Nicholas J.Millichamp, Yoshifumi Ninomiya, Yoshikazu Sado, and Youngki Kim.: "A model of autosomal recessive Alport syndrome in English cocker spaniel dogs." Kidney International. (in press.). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ueki Y,Naito I,Oohashi T,Sugimoto M,Seki T,Yoshioka H,Sado Y,Sato H,Sawai T,Sasaki F,Matsuoka M,Fukuda S and Ninomiya Y: "Topoisomerase I and II consensus sequences in a 17-kb deletion junction of the COL4A5 and COL4A6 genes and immunohistochemical analysis of esophageal leiomyomatosis associated with Alport syndrome." Am J Hum Genet. 62. 253-261 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Gunwar S., Ballester F., Noelken M.E., Sado Y., Ninomiya Y., and Hudson B.G.: "Glomerular Basement Membrane : Identification of a novel disulfide-crosslinked network of alpha3, alpha4 and alpha5 chains of type IV collagen and its implications for the pathogenesis of Alport syndrome." J.Biol.Chem.(in press.). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Heidet, L., Boye, E., Cai, Y., Sado, Y., Zhang, X., Flejou, J.-M., Fekete, F., Ninomiya, Y., Gubler, M.-C., Antignac, C.: "Somatic deletion of the 5'ends of both the COL4A5 and COL4A6 genes in a sporadic leiomyoma of the esophagus." Am J.Pathol.(in press.). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ono, S., Date, I., Nakajima, M., Onoda, K., Shiota, T., Ohmoto, T., Ninomiya, Y., and Asari, S.: "NF-kB decoy administration into the subarachnoid space as gene therapy for angiopathy. in May 1 issue, 1998" Human Gene Therapy. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Momota, R., Sugimoto, M., Oohashi, T., Kigasawa, K., Yoshioka, H., and Ninomiya, Y.: "Two genes, COL4A3 and COL4A4 coding for the human alpha3 (IV) and alpha4 (IV) collagen chains are arranged head-to-head on chromosome 2q36." FEBS Letters. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wu, Y.-L., Sumiyoshi, H., Khaleduzzaman, M., Ninomiya, Y., and Yoshioka, H.: "cDNA sequence and expression of the Mouse alpha1 (V) Collagen Gene (Col5a1)." Biochem.Biophys.Acta. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hargey, S.J.Zheng, K., Sado, Y., Naito, I., Ninomiya, Y., Jacobs, R.M., Hudson, B.G., THorner, P.S.: "The role of distinct type IV collagen networks in glomerular development and function. submitted for publication" Kidney International. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seki, T., Naito, I., Oohashi, T., Sado, Y., and Ninomiya, Y.: "Differential expression of type IV collagen isoforms, alpha5 (IV) and alpha6 (IV) chains, in basement membranes surrounding smooth muscle cells." Histochem Cell Biol. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshikazu Sado, Megumi Kagawa, Ichiro Naito, Yasuyoshi Ueki, Tsugio Seki, Ryusuke Momota, Toshitaka Oohashi, and Yoshifumi Ninomiya: "Organization and Expression of Basement Membrane Collagen IV Genes and their Roles in Human Disorders." J.Biochem.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
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      1997 Final Research Report Summary
  • [Publications] Megumi Kagawa et al.: "Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport's syndrome." Nephrol.Dial.Transplant.12. 1238-1241 (1997)

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  • [Publications] Sado Y et al.: "Purification and characterization of human nephritogenic antigen that induces anti-GBM nephritis in rats." J.Pathol.182. 225-232 (1997)

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  • [Publications] Heidet L et al.: "Diffuse leiomyomatosis associated with X-linked Alport syndrome : extracellular matrix study using immunohistochemistry and in situ hybridization." Lab.Invest.76(2). 233-243 (1997)

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  • [Publications] Tanaka K et al.: "Differential expression of α1(IV),α2(IV),α5(IV),α6(IV) collagen chains in the basement membrane of basal cell carcinoma." Histochem.J.29. 563-570 (1997)

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  • [Publications] Fleischmajer R et al.: "There is temporal and spatial expression of α1(IV),α2(IV),α5(IV),α6(IV) collagen chains and b integrins during the development of the basal lamina in an "in vitro" skin model." J.Invest.Dermatol.109(4). 527-533 (1997)

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  • [Publications] Olson M.W.et al.: "High affinity binding of Matrix metalloproteinase-9 to the pro-α2(IV) chain of collagen IV. Role in binding of zymogen to the surface of human breast epthelial MCF10A cells." J.Biol.Chem.(in press). (1998)

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  • [Publications] Yoshikazu Sado et al.: "Induction of anti-GBM nephritis in rats by recombinant α3(IV) and α4(IV)NCl of type IV collagen." Kidney International. (in press). (1998)

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      1997 Annual Research Report
  • [Publications] Ueki Y et al.: "Topoisomerase I and II consensus sequences in a 17-kb deletion junction of the COL4A5 and COL4A6 genes and immunohistochemical analysis of esopha geal leiomyomatosis associated with Alport syndrome." Am.J.Hum.Genet.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Gunwar S.et al.: "Glomerular Basement Membrane : Identification of a novel disulfide-crosslinked network of α3,α4 and α5 chains of type IV collagen and its implications for pathogenesis of Alport syndrome." J.Biol.Chem.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Heidet,L.et al.: "Somatic deletion of the 5'ends of both the COL4A5 and COL4A6 genes in a sporadic leiomyoma of the esophagus." Am J.Pathol.(in press). (1998)

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  • [Publications] Glew R.H.& Hinomiya Y.: "Clinical Studies in Medical Biochemistry" Oxford University Press New York, (1997)

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  • [Publications] Tryggvason K: "Alport Syndrome (Hereditary nephritis) in Clinical Studies in Medical Biochemistry" Oxford University Press,New York, 9 (1997)

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  • [Publications] Yoshikazu Sado et al.: "Expression of collagen IV genes and Goodpasture syndrome." ECM-Cell Interaction (in press),

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  • [Publications] Olsen B.R.& Ninomiya Y.: "Collagens In : Guide book to the extracellular matrix and adhesion proteins" Boca Raton FL,CRC Press (in press), (1997)

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  • [Publications] Sugihara K.: "Experimental Anti-GBM glomerular nephritis induced in rats byimmunization with syhthetic peptides based on six a chains of human type IV collagen." J.Pathol.178. 352-358 (1996)

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  • [Publications] Ljubimov A.V.: "Extracellular alterations in human corneas with bullous keratopathy." Invest.Ophthal mol.Vis.Sci.37. 997-1007 (1996)

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  • [Publications] Rauf S.: "Nephritogenicity and a-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane." Virchow Archiv. 428. 281-288 (1996)

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      1996 Annual Research Report
  • [Publications] Hino S: "Absence of α6(IV) collagen in kidney and skin of X-linked Alport syndrome." Pediatric Nephrology. 10. 742-744 (1996)

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      1996 Annual Research Report
  • [Publications] Ljubinov,A V: "Basement membrane abnormalities in human eye with diabetic retinopathy." J.Histochem.Cytochem.44. 1469-1479 (1996)

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  • [Publications] Megumi Kagawa: "Epitope-defined monoclonal antibodies against type-IV collagen for diagnosis of Alport's syndrome. (in press)" Nephrology Dialysis Transplantation. (1997)

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  • [Publications] Glew R.H.and Ninomiya Y.: "Clinical Studies in Medical Biochemistry" Oxford University Press,New York (in press), 1-449 (1997)

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  • [Publications] 二宮善文,渡辺明治,岡崎勲: "遺伝子とその発現 細胞外マトリックス-臨床医学への応用-" メヂカルレビュー社, (1996)

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Published: 1996-04-01   Modified: 2016-04-21  

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