| Project/Area Number |
08457162
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
YOKOSUKA Osamu CHIBA UNIVERSITY,FACULTY OF MEDICINE,LECTURER, 医学部, 講師 (90182691)
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| Co-Investigator(Kenkyū-buntansha) |
TAGAWA Masami CHIBA UNIVERSITY,FACULTY OF MEDICINE,ASSISTANT, 医学部付属病院, 助手 (90261916)
IMAZEKI Fumio CHIBA UNIVERSITY,FACULTY OF MEDICINE,LECTURER, 医学部, 講師 (40223325)
江畑 稔樹 千葉大学, 医学部・付属病院, 助手 (20280919)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | Hepatitis A virus / fulminant hepatitis / mutation / sequence / Hepatis A / 劇症肝炎 / 塩基配列決定 / ウイルス変異 |
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| Research Abstract |
Hepatitis A still possess a considerable problem not only in underdeveloped but also in developed countries. It is not revealed why some patients progress to fulminant hepatitis A and others do not. To examine whether genomic differences of hepatitis A virus (HAV) are responsible for the clinical severity, we are analyzing whole nucleotide sequences of HAV RNA in sera from fulminant and acute hepatitis A patients. We clarified that the severity of hepatitis A appears to be associated with the substitutions near the central part of the 5' nontranslated region of HAV,because in the cases with fulminant hepatitis only a small number of substitutions were observed while in the cases with self limited acute hepatitis considerable numbers of substitutions were observed. We further determined the sequences of structural protein region of HAV.Sera from 3 fulminant and 3 acute hepatitis A patients were examined. Structural region and non-strauctural regions of HAV RNA were amplified by long reverse transcription-polymerase chain reaction (RT-PCR) devided into 3188 bp including structural protein region and 4688 bp including non-structural protein region. Each PCR product was cloned and the nucleotide sequence of structural protein region was sequenced by automated sequencer. The genotypes of examined cases were all genotype1A.There were no such obvious differeces in structural protein region as found in 5' nontranslated region between fulminant and acute hepatitis A.We are now examining the sequences of non-structural protein region of HAV.
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