| Project/Area Number |
08457171
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Miyazaki Medical College |
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Principal Investigator |
TSUBOUCHI Hirohito Miyazaki Medical College, Second Department of Internal Medicine, Professor, 医学部, 教授 (60145480)
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| Co-Investigator(Kenkyū-buntansha) |
HIRONO Shuichi Miyazaki Medical College, Second Department of Internal Medicine, Lecturer, 医学部, 講師 (00181222)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | HGF / HGF ACTIVATOR / LIVER INJURY / GASTRIC MUCOSAL INJURY / 成熟型HGF / 臓器特異性 / 肝障害モデル / 胃粘膜障害モデル |
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| Research Abstract |
We examined the expression of HGF and HGF activator in various organs of carbon tetrachloride (CCl_4)-treated Japanese monkeys and hydrochloric acid (HCl)-administered Japanese monkeys. Japanese monkeys with hepatic injury and gastric mucosal injury were prepared by administration of single intragastric dose of CCl_4 in olive oil and HCl, respectively. HGF and HGF activator protein levels were determined using ELISA for human HGF and HGF activator. Heterogeneity of HGF in the tissues were measured by Western blot analysis using anti-HGF monoclonal antibody. HGF and HGF activator mRNAs were analyzed by northern blot analysis using their cDNAs.
Marked increase in HGF protein level was observed in the liver of CCl_4-treated monkeys, and its slight increase in the spleen. Not only proHGF but also mature HGF increased in those tissues. Increased expression of HGF mRNA level was also found in those tissues. However, there is no significant increase in HGF protein and HGF mRNA in other tissues including kidney and lung. HGF activator and its mRNA was detected only in the liver of both normal and CCl_4-treated monkeys. In addition, activity which processed proHGF to mature HGF was only detected in the CCl_4-injured liver.
HGF protein and HGF mRNA expression in gastric mucosa of HCl-treated monkeys markedly increased. Increase in both proHGF and mature HGF was found in the HCl-injured gastric mucosal tissue and HGF activator mRNA was detected only in the liver as well as CCl_4-treated monkeys.
These results suggest that HGF appears in the liver of monkeys with hepatic injury and in the gastric mucosa of monkeys with gastric mucosal injury that is injured-tissue specific.
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