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ANTISENSE HOMOLOGY BOX THEORY FOR INHIBITION OF GROWTH FACTOR RECEPTOR IN GASTROINTESTINAL TRACT

Research Project

Project/Area Number 08457172
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionNAGOYA CITY UNIVERSITY

Principal Investigator

ITOH Makoto  NAGOYA CITY UNIVERSITY.FIRST DEPARTMENT OF INTERNAL MEDICINE.PROFESSOR, 医学部, 教授 (00080119)

Co-Investigator(Kenkyū-buntansha) KATO Taiji  NAGOYA CITY UNIVERSITY.FIRST DEPARTMENT OF INTERNAL MEDICINE.PROFESSOR, 医学部, 教授 (60094364)
JOH Takashi  NAGOYA CITY UNIVERSITY.FIRST DEPARTMENT OF INTERNAL MEDICINE.ASSISTANT PROFESSOR, 医学部, 講師 (30231369)
OKDA Hidetika  NAGOYA CITY UNIVERSITY.FIRST DEPARTMENT OF INTERNAL MEDICINE.PROFESSOR, 医学部, 教授 (30160683)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsGROWTH FACTOR / ANTISENSE PEPTIDE / INHIBITOR / EGF / RECEPTOR / 成長因子
Research Abstract

In this study, biologically active growth modulators in human gastric juice and antisense homology box theory (Nature Med 1 : 894-901,1995) for making inhibitor of growth factor action were evaluated. Mitogenic activity was evaluated by the incorporation of [^3H] thymidine into 3T3 fibroblasts. A negative correlation was observed between pH and mitogenic activity in gastric juice. The concentrations of epidermal growth factor (EGF), transforming growth factor-a &-b1 (TGF-a & b1), platelet-derived growth factor (PDGF), and basic fibroblast growth factor (bFGF) in gastric juice did not explain these changes in mitogenic activity. Gel filtration identified growth stimulating activity due to small molecule mitogens (less than 13 kDa), and growth inhibitory activity in only neutral samples due to a macromolecular substance (lager than 240 kDa) susceptible to trypsin digestion, heat-and acid-treatments. EGF receptor molecule was analyzed by our software on IBM computer to determine the number and distribution of intramoecular regions having sense-antisense relationships in randomly selected sets of protein. According to this analysis, following peptide was synthesized ; PSGTARTKLLL,LAALCAAGGALEEKKV,LYENTYALAVLSNYG,DSLSINA,NGIGIGE,DPRELEILKTVKEITGFLLI,LHILPVAFK-GDSFTRTPPL,VERIPLENLQIIRGNALY,RGRTKQHGQFSLAVVGLN,HNQSAAGSTGPRESDAL,LVWKFADANNVAHLSHA,QYYLESIRV-LERVKYIR,YVVTDHGSAVRASG,LRSLKEISDGDVI,QITKLLDG-TSIYY.Biological activity of these peptide was evaluated by measuring incorporation of [^3H] thymidine into RGM1 (gastric epithelium), and intracellular concentration of Ca^<++> of rat hepatocyte. DPRELEILKTVKEITGFLLI enhanced action of EGF,but others did not show significant actions, which may indicate these peptide need the flanking regions for increasing biological activity.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Kunio Kasugai, Takashi Joh Taiji Kato et al: "Effect of anti-gastrio secretory drugs,IT-066 and omeprazole,on mitogenic activities in gastric juice of the rat" Scand.J.Gastroenterol. 31. 111-117 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kunio Kasugai, Takashi Joh Hiromi Kataoka et al: "Evidences for Participation of Gliostatin/Platelet-Perived Endothelial Cell Growth Factor in Gastric Ulcer Healing" Life Science. 61. 1899-1906 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiromi Kataoka, Takashi Joh Kunio Kasugai et al: "Mitogenic properties of human gastric juice" Dig.Pis.Sci. 42. 1747-1754 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kunio Kasugai, Takashi Joh, Taiji Kato, Makoto Itoh: "Effect of anti-gastric secretory drugs, IT-066 and omeprazole, on mitogenic activities in gastric juicc of the rat." Scand.J.Gastroenterol.31. 111-117 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kunio Kasugai, Takashi Joh, Hiromi Kataoka, Makoto Sasaki, Toyohiro Tada, Kiyofumi Asai, Taiji Kato, Makoto Itoh.: "Evidences for Participation of Gliostatin/Platelet-Derived Endothelial Cell-Growth Factor in Gastric Ulcer Healing." Life Science. 61. 1899-1906 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiromi Kataoka, Takashi Joh, Kunio Kasugai, Taiji Kato, and Akihiko moriyama.: "Mitogenic propcrties of human gastric juice." Dig.Dis.Sci.42. 1747-1754 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kunio Kasugai, et al: "Evidences for Participation of Gliostatin Platelet Derived Endothelial Cell Growth Factor in Gastric Ulcer Healing" Life Science. 61. 1899-1906 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiromi Kataoka, et al: "Mitogenic properties of human gastric juice" Dig.Dis.Sci. 42. 1747-1754 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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