| Project/Area Number |
08457175
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | JUNTENDO UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
SATO Nobuhiro JUNTENDO UNIV.SCHOOL OF MEDICINE PROFESSOR, 医学部, 教授 (90028358)
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| Co-Investigator(Kenkyū-buntansha) |
HIROSE Miyoko HUNTENDO UNIV.SCHOOL OF MEDICINE, 医学部, 助手 (70266039)
OIDE Hirosumi JUNTENDO UNIV.SCHOOL OF MEDICINE, 医学部, 講師 (80223725)
MIWA Hiroto JUNTENDO UNIV.SCHOOL OF MEDICINE, 医学部, 講師 (80190833)
WATANABE Sumio JUNTENDO UNIV.SCHOOL OF MEDICINE ASSOCIATE PRORESSOR, 医学部, 助教授 (20138225)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | migration / proliferation / apotosis / Helicobacter pylori / bile acid / alcohol / radical / wound healing / Apoptosis |
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| Research Abstract |
The wound repair of gastric mucosal cells in vitro (i.e.the ability of cultured cells to fill the artificially made cell-free area) requires activation of both cell migration and proliferation.
However, in this model, the role of cell migration is much more important than that of proliferation because more than three-fourths of wound repair occurred before detection of BrdU-positive cells. Most of the process, especially the initial stage of repair, is completed by only cell migration, and only the late stage might include cell proliferation. Factors which might modulate the healing process have been investigated. In a series of experiments, we examined the effects of a variety of available growth factors. Addition of growth factors such as hepatocyte growth factor induced wound repair in the monolayr gastric epithelial cell sheet. In this project, we assessed the effects of temperature, free radicals and Helicobacter pylori water extract on the process of restoration using above wound repair model. Free radical inducer such as bile acids, ethanol and H.pylori extract retarded the restoration process including cell proliferation and migration with the induction of apoptosis. Especially, toxin in H.pylori water extract used in this experiment is different from vacA gene products and cagA gene products. Further study must be done to identify the character of this new toxin.
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